Rapid Communication in Photoscience

Korean Society of Photoscience (한국광과학회)
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Characterization of LexA-mediated Transcriptional Enhancement of Bidirectional Hydrogenase in Synechocystis sp. PCC 6803 upon Exposure to Gamma Rays

  • Kim, Jin-Hong (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Lee, Min Hee (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Ji Hong (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Moon, Yu Ran (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Cho, Eun Ju (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Ji Eun (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Lee, Choon-Hwan (Department of Molecular Biology, Pusan National University) ;
  • Chung, Byung Yeoup (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
  • Received : 2012.01.04
  • Accepted : 2012.01.30
  • Published : 2012.03.01
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Abstract

Influence of gamma rays on the cyanobacterium Synechocystis sp. PCC 6803 cells was investigated in terms of a bidirectional hydrogenase, which is encoded by hoxEFUYH genes and responsible for biohydrogen production. Irradiated cells revealed a substantial change in stoichiometry of photosystems at one day after gamma irradiation at different doses. However, as evaluated by the maximal rate of photosynthetic oxygen evolution, maximal photochemical efficiency of photosystem II, and chlorophyll content, net photosynthesis or photosynthetic capacity was not significantly different between the control and irradiated cells. Instead, transcription of hoxE, hoxH, or lexA, which encodes a subunit of bidirectional hydrogenase or the only transcriptional activator, LexA, for hox genes, was commonly enhanced in the irradiated cells. This transcriptional enhancement was more conspicuously observed immediately after gamma irradiation. In contrast, hydrogenase activities were found to somewhat lower in the irradiated cells. Therefore, we propose that transcription of hox genes should be enhanced by gamma irradiation in a LexA-mediated and possibly photosynthesis-independent manner and that this enhancement might not induce a subsequent increase in hydrogenase activities, probably due to the presence of post-transcriptional and/or post-translational regulatory mechanisms.

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References

  1. Antal, T. K.; Oliveira, P.; Lindblad, P. Int. J. Hydrogen Energ. 2006, 31, 1439-1444. https://doi.org/10.1016/j.ijhydene.2006.06.037
  2. Gutekunst, K.; Phunpruch, S.; Schwarz, C.; Schuchardt, S.; Schulz-Friedrich, R.; Appel, J. Mol. Microbiol. 2005, 58, 810-823. https://doi.org/10.1111/j.1365-2958.2005.04867.x
  3. Axelsson, R.; Lindblad, P. Appl. Environ. Microbiol. 2002, 68, 444-447. https://doi.org/10.1128/AEM.68.1.444-447.2002
  4. Sheremetieva, M.; Troshina, O.; Serebryakova, L. T.; Lindblad, P. FEMS Microbiol. Lett. 2002, 214, 229-233. https://doi.org/10.1111/j.1574-6968.2002.tb11352.x
  5. Antal, T. K.; Oliveira, P.; Lindblad, P. J. Appl. Microbiol. 2005, 98, 114-120. https://doi.org/10.1111/j.1365-2672.2004.02431.x
  6. Oliveira, P.; Lindblad, P. FEMS Microbiol. Lett. 2005, 251, 59-66. https://doi.org/10.1016/j.femsle.2005.07.024
  7. Domain, F.; Houot, L.; Chauvat, F.;Cassier-Chauvat, C. Mol. Microbiol. 2004, 53, 65-80. https://doi.org/10.1111/j.1365-2958.2004.04100.x
  8. Appel, J.; Phunpruch, S.; Steinm?ller, K.; Schulz. R. Arch. Microbiol. 2000, 173, 333-338. https://doi.org/10.1007/s002030000139
  9. Murakami, A. Photosynth. Res. 1997, 53, 141-148. https://doi.org/10.1023/A:1005818317797
  10. Song, E. K.; Zulfugarov, I. S.; Kim, J.-H.; Kim, E. H.; Lee, W. S.; Lee, C.-H. J. Plant Biol. 2004, 47, 289-299.
  11. Gutthann, F.; Egert, M.; Marques, A.; Appel, J. Biochim. Biophys. Acta 2007, 1767, 161-169. https://doi.org/10.1016/j.bbabio.2006.12.003
  12. Miki, T.; Shirabe, K.; Ebina, Y.; Nakazawa, A. Nucleic Acids Res. 1984, 12, 1203-1217.
  13. Patterson-Fortin, L. M.; Colvin, K. R.; Owttrim, G. W. Nucleic Acids Res. 2006, 34, 3446-3454. https://doi.org/10.1093/nar/gkl426
  14. Moon, Y. R.; Lee, M. H.; An, B. C.; Chung, B. Y.; Kim, J.- S.; Park, Y.-I.; Kim, C. S.; Kim, J.-H. J. Radiat. Ind. 2009, 3, 107-114.
  15. Schmittgen, T. D.; Zakrajsek, B. A.; Mills, A. G.; Gorn, V.; Singer, M. J.; Reed, M. W. Anal. Biochem. 2000, 285, 194-204. https://doi.org/10.1006/abio.2000.4753
  16. Tamagnini, P.; Leit o, E.; Oliveira, P.; Ferreira, D.; Pinto, F.; Harris, D. J.; Heidorn, T.; Lindblad, P. FEMS Microbiol. Rev. 2007, 31, 692-720. https://doi.org/10.1111/j.1574-6976.2007.00085.x