Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Anti-oxidant activity of Phenolic Compound Isolated from the Fruits of Acanthopanax sessiliflorus Seeman

오가피(Acanthopanax sessiliflorus Seeman) 열매로부터 분리한 페놀 화합물의 항산화활성

  • In, Seo-Ji (Graduate School of Biotechnology & Department of Oriental Medicinal Materials and Processing, Kyung Hee University) ;
  • Lee, Dae-Young (Herbal Crop Utilization Research Team, National Institute of Horticultural and Herbal Science, RDA) ;
  • Seo, Kyeong-Hwa (Graduate School of Biotechnology & Department of Oriental Medicinal Materials and Processing, Kyung Hee University) ;
  • Nam, Tae-Gyu (Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Kim, Dae-Ok (Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Kim, Geum-Soog (Herbal Crop Utilization Research Team, National Institute of Horticultural and Herbal Science, RDA) ;
  • Noh, Hyung-Jun (Herbal Crop Utilization Research Team, National Institute of Horticultural and Herbal Science, RDA) ;
  • Kim, Gye-Won (Brewing Research Center, Hankyung National University) ;
  • Seo, Woo-Duck (Department of Functional Crop, National Institute of Crop Science, RDA) ;
  • Kang, Hee-Cheol (GFC Co., Ltd.) ;
  • Baek, Nam-In (Graduate School of Biotechnology & Department of Oriental Medicinal Materials and Processing, Kyung Hee University)
  • Received : 2012.10.16
  • Accepted : 2012.12.04
  • Published : 2012.12.31
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Abstract

The fruits of Acanthopanax sessiliflorus Seeman (Araliaceae) were extracted with 70% aqueous ethanol at room temperature. The concentrated extract was partitioned with ethyl acetate (EtOAc), n-butyl alcohol, and $H_2O$, successively. From the EtOAc fraction, two compounds were isolated through the repeated silica gel, octadecyl silica gel, and Sephadex LH-20 column chromatographies. According to the results of physicochemical and spectroscopic data including NMR, mass spectrometry, and infrared spectroscopy, the chemical structures of the compounds were determined as 3,5-dihydroxycinnamic acid (1) and protocatechuic acid (2). Compound 1 was isolated from the fruits of A. sessiliflirus Seeman for the first time. And the compounds were evaluated for the radical scavenging the antioxidant capacity using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)diammonium salt, 1,1-diphenyl-2-picrylhydrazy, and oxygen radical absorbance capacity assay.

오가피(Acanthopanax sessiliflorus Seeman) 열매를 실온에서 70% ethanol (EtOH)로 추출하고 이 추출물을 ethyl acetate (EtOAc) 분획, n-butyl alcohol 분획, $H_2O$ 분획으로 나누었다. EtOAc 분획에 대하여 silica gel, octadecyl silica gel 및 Sephadex LH-20 column chromatography를 반복 실시하여 2종의 화합물을 분리, 정제하였다. NMR, infrared spectroscopy, 및 electron ionization/mass spectrometry 등의 spectrum을 해석하여, 화합물 1과 화합물 2를 각각 3,5-dihydroxycinnamic acid과 protocatechuic acid 로 구조를 결정하였다. 화합물 1은 오가자에서는 처음으로 분리된 화합물이다. 또한 이 화합물에 대한 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)diammonium salt, 1,1-diphenyl-2-picrylhydrazyl 및 oxygen radical absorbance capacity radical 소거능을 이용한 항산화 활성을 측정하였는데, 모두 vitamin C보다 2배 이상 활성이 높은 것으로 나타났다.

Keywords

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