Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Antioxidant Activities of Extracts from Medicinal Plants

약용식물 추출물의 항산화 활성

  • Park, Hye-Jin (School of Applied Biosciences, Kyungpook National University) ;
  • Kang, Sun-Ae (School of Applied Biosciences, Kyungpook National University) ;
  • Lee, Ju-Yeong (School of Applied Biosciences, Kyungpook National University) ;
  • Cho, Young-Je (School of Food Science & Biotechnology, Food & Bio-Industry Research Institute, Kyungpook National University)
  • 박혜진 (경북대학교 응용생명과학부) ;
  • 강선애 (경북대학교 응용생명과학부) ;
  • 이주영 (경북대학교 응용생명과학부) ;
  • 조영제 (경북대학교 식품공학부/식품생물산업연구소)
  • Received : 2012.07.20
  • Accepted : 2012.09.28
  • Published : 2012.10.30
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Abstract

In this study, the optimal conditions for phenolic-compound extraction from medicinal plants were found to be 24 h and about 50% ethanol. The electron-donating scavenging activities (DPPH), ABTS radical-cation decolorization (ABTS), antioxidant protection factor (PF), and thiobarbituric acid reaction substance (TBAR) were measured to determine the antioxidant activities of the extracts of Sanguisorba officinalis Linn., Citrus unshiu Markovich, Melia azedarach L., Asparagus cochinchinensis Merr., Citrus unshiu S., Polygonum aviculare L., and Leonurus sibiricus L. The total phenolic contents of the extracts of medical plants were determined to be 0.45-3.00 mg/g in the water extracts and 0.33-3.15 mg/g in the 50% ethanol extracts. The electron-donating abilities (EDA) of the water and ethanol extracts were both above 85% at the $50{\mu}g/ml$ concentration. The ABTS radical-cation decolorization was above 80% at the $100{\mu}g/ml$ concentration in all the extracts of various medicinal plants. The antioxidant protection factor (PF) of the Melia azedarach L. extracts was found to be $1.65{\pm}0.40$ PF in the water extracts at the $100{\mu}g/ml$ concentration, and was higher than those of the other medicinal-plant extracts. The TBAR inhibition rates of all the medicinal-plant extracts, except Asparagus cochinchinensis Merr., were above 85% at the $100{\mu}g/ml$ concentration. These results confirmed that the various oriental medicinal plants (Sanguisorba officinalis Linn., Citrus unshiu Markovich, Melia azedarach L., Asparagus cochinchinensis Merr., Citrus unshiu S., Polygonum aviculare L., and Leonurus sibiricus L.) that were included in this study are useful functional-food sources.

안전한 천연물을 이용한 천연항산화제 개발 연구에 대한 관심이 고조되는 가운데, 지유, 진피, 천련자, 천문동, 청피, 편축, 하고초 7종의 천연 약용식물 추출물을 통해 항산화 활성을 측정하였다. 추출물의 총 페놀 함량은 지유가 열수추출물과 40% ethanol 추출물에서 각각 31.5 mg/g, 32.7 mg/g으로 가장 높게 나타났다. 추출물의 수용성 물질에 대한 항산화 효과는 DPPH radical 소거능이 지유, 진피, 천련자, 천문동, 청피, 편축, 하고초 7종의 천연 약용식물의 열수 추출물과 ethanol 추출물에서 80% 이상의 높은 소거활성을 나타내었다. ABTS radical cation decolorization을 측정한 결과, 7종의 천연 약용식물의 열수 추출물과 ethanol 추출물에서 85% 이상의 높은 저해율을 나타내어 매우 우수한 항산화 효과를 가지고 있음을 입증하였다. 지용성 물질에 대한 항산화 효과를 측정하기 위해 Antioxidant Protection Factor(PF)를 측정한 결과, 천련자 열수 추출물에서 1.65 PF로 대조구인 vitamin C 보다 높은 수준을 나타내어 지용성 물질에 대한 항산화 활성이 높음을 확인하였다. TBARs 생성 저해율을 측정한 결과 지유, 천련자, 청피, 편축, 하고초의 열수 추출물과 ethanol 추출물에서 99%에 가까운 높은 저해율을 나타내어 대조구인 vitamin C보다 지용성 물질에 대한 항산화 활성이 매우 높은 것으로 확인되었다.

Keywords

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