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A Phylogenetic Relationship between Foreign and Korean Strains of Flammulina velutipes Identified by rDNA-ITS Sequence Analysis

Flammulina velutipe의 국내 균주와 외래 균주 간의 ITS region을 이용한 계통학적 유연관계 분석

  • Hwang, Gwang-Rip (Department of Life Sciences and Biotechnology, Kyungpook National University) ;
  • Woo, Ju-Ri (Department of Life Sciences and Biotechnology, Kyungpook National University) ;
  • Yoon, Hyeok-Jun (Department of Life Sciences and Biotechnology, Kyungpook National University) ;
  • Lee, Chang-Yun (Greenpeace Mushroom Co.) ;
  • Lee, Sang-Han (Food & Bio-Industry Research Institute and Department of Food Science & Technology) ;
  • Kong, Won-Sik (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Kim, Jong-Guk (Department of Life Sciences and Biotechnology, Kyungpook National University)
  • 황광립 (경북대학교 생명과학부) ;
  • 우주리 (경북대학교 생명과학부) ;
  • 윤혁준 (경북대학교 생명과학부) ;
  • 이창윤 (그린피스) ;
  • 이상한 (경북대학교 식품생물산업연구소 및 식품공학과) ;
  • 공원식 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 김종국 (경북대학교 생명과학부)
  • Received : 2011.11.03
  • Accepted : 2012.01.18
  • Published : 2012.01.30

Abstract

This study was carried out to investigate the genetic relationship of Flammulina velutipes with other species. The ribosomal DNA cluster containing 4 rRNA genes from F. velutipes 4154 were sequenced. The length of the rDNA cluster sequence was estimated at 7,403 bp long and consisted of 1,806 bp of SSU rDNA, 245 bp of ITS 1 region, 159 bp of 5.8S rDNA, 308 bp of ITS 2 region, 3,402 bp of LSU rDNA, 1,400 bp of IGS 1 region, and 83 bp of 5S rDNA. The F. velutipes 4154 genes were contained in the rDNA cluster of F. velutipes in the order of SSU rDNA - ITS 1 - 5.8S rDNA - ITS 2 - LSU rDNA - IGS 1 - 5S rDNA. The phylogenetic relationships of 20 strains of Tricholomataceae and Physalacriaceae were analyzed by conducting distance analysis using the Neighbor-joining (NJ) method. The 20 strains used in this study were divided into three groups and the strains of the genus Flammulina were related very closely to strains of Physalacria bambusae.

Acknowledgement

Grant : 버섯수출사업단 및 바이오그린21 연구지원사업

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