Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Interaction of Apidaecin Ib with Phospholipid Bilayers and its Edwardsiella Species-specific Antimicrobial Activity

  • Seo, Jung-Kil (Department of Biotechnology, Pukyong National University) ;
  • Go, Hye-Jin (Department of Biotechnology, Pukyong National University) ;
  • Moon, Ho-Sung (Department of Biotechnology, Pukyong National University) ;
  • Lee, Min-Jeong (Department of Biotechnology, Pukyong National University) ;
  • Hong, Yong-Ki (Department of Biotechnology, Pukyong National University) ;
  • Jeong, Hyun-Do (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Nam, Bo-Hye (Biotechnology Research Division, Aquaculture Industry Department, National Fisheries Research and Development Institute) ;
  • Park, Tae-Hyun (West Vancouver Secondary School) ;
  • Park, Nam-Gyu (Department of Biotechnology, Pukyong National University)
  • Received : 2011.10.05
  • Accepted : 2011.11.10
  • Published : 2012.01.20
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Abstract

Apidaecin Ib had strong antimicrobial activity against several tested Gram-negative bacteria including Escherichia coli, Enterobacter cloacae, and Shigella flexneri (MECs; $0.3-1.5{\mu}g/mL$), but showed no activity against all the tested Gram-positive bacteria including Bacillus subtilis, Micrococcus luteus, Staphylococcus aureus and one yeast, Candida albicans (MECs; > $125{\mu}g/mL$). Interestingly, this peptide showed potent antibacterial activity only against Edwardsiella species (MECs; $0.6-3.6{\mu}g/mL$) among the tested fish pathogenic bacteria through a bacteriostatic process and showed no significant hemolytic activity. Apidaecin Ib took an unordered structure in all environments and also had very weak membrane perturbation activity even at $25{\mu}M$. Anti-Edwardsiella activity of apidaecin Ib is stronger than those of other antimicrobial polypeptides or antibiotics, but its activity is salt-sensitive. These results suggest that apidaecin Ib has Edwardsiella speciesspecific antibacterial activity and could be applied as new preventive or control additives for Edwardsiella species infection in freshwater fish aquaculture.

Keywords

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