Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지
DOI QR코드

DOI QR Code

Antithrombotic Effect and Antiplatelet Activity of Polygonum cuspidatum Extract

호장근 추출물의 항혈전 효능 및 혈소판 응집 억제작용

  • Yang, Won-Kyung (Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine) ;
  • Sung, Yoon-Young (Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine) ;
  • Kim, Ho-Kyoung (Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine)
  • 양원경 (한국한의학연구원 한약기초연구그룹) ;
  • 성윤영 (한국한의학연구원 한약기초연구그룹) ;
  • 김호경 (한국한의학연구원 한약기초연구그룹)
  • Received : 2011.10.17
  • Accepted : 2012.01.06
  • Published : 2012.02.29
Download PDF
⟨ Previous Next ⟩

Abstract

We performed this study to develop antithrombotic agents from oriental medicine herb extracts. Polygonum cuspidatum has been traditionally used as an edible medical resources for the treatment of cancer, pyodermatitis, hepatitis, cystitis, and inflammation. However, the effects of Polygonum cuspidatum on thrombosis and platelet activation are not precisely understood. The antithrombotic and antiplatelet activities of Polygonum cuspidatum were investigated by assessing the effect of a 70% ethanol extract of Polygonum cuspidatum on blood coagulation, fibrinolysis, and platelet aggregation. Polygonum cuspidatum showed effective fibrinolytic activity at 10 mg/mL. Polygonum cuspidatum also inhibited adenosine diphosphate induced platelet aggregation. Furthermore, evaluation of anticoagulant activity showed that an extract of Polygonum cuspidatum prolonged coagulation time via activated partial thromboplastin time (APTT). Our results show that Polygonum cuspidatum can be a potential candidate for antiplatelet activity as well as a fibrinolytic agent.

항혈전 효능을 탐색하기 위하여 한약재의 호장근 추출물로 혈전 용해능 활성과 혈액 응고시간 지연효과 즉 PT(prothrombin time), APTT(activated partial thromboplastin time)와 혈소판 응집억제 활성 등에 대해 항혈전 효능을 평가하였다. 혈전용해도를 측정하는 fibrin plate가 용해되어 형성된 투명환의 넓이를 측정하는 실험을 진행한 결과 혈전 용해도가 농도의존적으로 탁월한 효능을 나타내었다. 혈액응고 cascade에 미치는 영향을 알아보기 위해 혈액 응고시간 지연 및 단축 효과를 확인하고자 APTT와 PT에 미치는 영향을 조사한 결과 PT의 경우 대조군보다 단축됨을 보였다. APTT의 경우 농도의존적으로 우수한 지연효과를 보였다. 혈소판의 응집에 따라 형성되는 두 전극 사이에 형성된 전기적 저항의 변화로 나타나는 실험을 시행한 결과 호장근의 ADP로 유도한 결과에서 뛰어난 응집억제 활성을 보였다. 따라서 호장근을 향후에 혈전 질환의 치료제 개발에 효과적으로 이용될 수 있을 것으로 사료된다.

Keywords

References

  1. Kim YW, Kim DC. 1999. Evaluation of thrombolytic effect of streptokinase-dextran conjugate in a rat model of arterial thrombosis. Korean J Pharm Sci 29: 211-216.
  2. Yun YP, Kang WS, Lee MY. 1996. The antithrombotic effect of green tea catechins. Korean J Food Hyg Safety 11: 77-82.
  3. Lee HS. 1995. How safe is the readministration of streptokinase? Drug Saf 13:76-80. https://doi.org/10.2165/00002018-199513020-00002
  4. Kim JY, Yang KS. 1999. Antilipoperoxidant activity of the Polygonum cuspidatum on carbon tetrachloride induced hepatotoxicity. Yakhak Hoeji 43: 572-576.
  5. Lee TK, Kim JH, So JN. 2003. Emodin from Polygonum cuspidatum showed angiogenesis inhibiting activity in vitro. Korean J Soc Agric Chem Biotechnol 46: 50-54.
  6. Kim JM, Cha DS, Jeon SR, Jeon H, Lim JP, Choi H, Lee KJ, Kang MS, Na HJ, Kim MY, Leem KH, Kim HC, Bu YM. 2008. Neuroprotective effect of the roots of Polygonum cuspidatum on transient focal cerebral ischemia in rats. Korean J Herbology 23: 225-233.
  7. Rho TC, Choi HC, Lee SW, Kim YH, Rho M, Kim YK, Lee HS. 2001. Inhibition of oxide synthesis by cumarins from Polygonum cuspidatum in LPS activated RAW 264.7 cells. Korean J Pharmacogn 32: 181-188.
  8. Jayasuriya H, Koonchanok NM, Geahlen RL, McLaughlin JL, Chang CJ. 1992. Emodin, a protein kinase inhibitor from Polygonum cuspidatum. J Nat Prod 55: 696-698. https://doi.org/10.1021/np50083a026
  9. Chang J, Liu H, Wang K, Chen M, Chiang L, Hua Y, Lin C. 2005. Ethanol extract of Polygonum cuspidatum inhibits hepatitis B birus in a stable HBV-producing cell line. Antiviral Reserch 66: 29-34. https://doi.org/10.1016/j.antiviral.2004.12.006
  10. Jeon WK, Kim JH, Lee AY, Kim HK. 2003. Inhibition of whole blood platelet aggregation from traditional medicine. Korean J Oriental Medicine 9: 55-67.
  11. Sohn HY, Son KH, Kwon CS, Kwon GS. 2007. A compound comprising 2α-hydroxy-oleanolic acid for prevention and control of thrombosis. Korean Patent 1007040030000.
  12. Fan J, Zhang Y, Chang X, Zhang B, Jiang D, Saito M, Li Z. 2009. Antithrombotic and fibrinolytic activities of methanolic extract of aged sorghum vinegar. J Agric Food Chem 57: 8683-8687. https://doi.org/10.1021/jf901680y
  13. Liu S, Jiang S, Wu Z, Lv L, Zhang J, Zhu Z, Wu S. 2002. Identification of inhibitors of the HIV-1 gp41 six-helix bundle formation from extracts of Chinese medicinal herbs Prunella vulgaris and Rhizoma cibotte. Life Sci 71: 1779-1791. https://doi.org/10.1016/S0024-3205(02)01939-2
  14. Birk S, Kruuse C, Peter KA. 2006. The headache-inducing effect of cilostazol in human volunteers. Cephalagia 26: 1304-1309. https://doi.org/10.1111/j.1468-2982.2006.01218.x
  15. De Meyer SF, Vanhooelbbeke K, Broos Kl. 2008. Antiplatelet drugs. Br J Haematol 142: 515-528. https://doi.org/10.1111/j.1365-2141.2008.07233.x
  16. Francescone S, Halperin JL. 2008. "Triple theraphy" or triple threat?: balancing the risks of antithrombotic therapy for patients with atrial fibrillation and coronary stents. J Am Coll Cardiol 51: 826-827. https://doi.org/10.1016/j.jacc.2007.11.034
  17. Fontanay S, Grare M, Mayer J, Finance C, Duval RE. 2008. Ursolic, oleanolic and betulinic acids: antibacterial spectra and selectivity indexs. J Ethnopharmacol 120: 272-276. https://doi.org/10.1016/j.jep.2008.09.001
  18. Liu X, Wu L, Song G, Liu K, Zhang X, Zhang Y. 1999. Study on chemical constituents of Polygonum cuspidatum Sieb. Shenyang Yaoke Daxue Xuebao 16: 17-20.
  19. Park CS, Lee YC, Kim JD, Kim CH. 2004. Inhibitory effects of Polygonum cuspidatum water extract (PCWE) and its component rasveratrol on acyl-coenzyme A-cholesterol acyltransferase activity for cholesteryl ester synthesis in HepG2 cells. Vasc Pharmacol 40: 279-284. https://doi.org/10.1016/j.vph.2004.01.003
  20. Kim YS, Kim HH, Yoo MJ, Shin DH. 2004. Bactericidal effect of the extracts of Polygonum cuspidatum on Bacillus cereus. Korean J Food Sci Technol 13: 430-433.
  21. Kimora Y, Okuda H, Arichi S. 1985. Effects of stilbene on arachidonate metabolism in leukocytes. Biochim Biophys Acta 834: 275-278. https://doi.org/10.1016/0005-2760(85)90167-5
  22. Agarwal SK, Singh SS, Verma S, Kumar S. 2000. Antifungal activity of anthraquinone derivatives from Rheum emodi. J Ethnopharmacol 72: 43-46. https://doi.org/10.1016/S0378-8741(00)00195-1
  23. Jayatilake GS, JAyasuriya H, Lee ES, Koonchanok NM, Geahien RL, Ashendel CL, McLaughlin JL, Chang CJ. 1993. Kinase inhibitors from Polygonum cuspidatum. J Nat Prod 56: 1805-1810. https://doi.org/10.1021/np50100a021
  24. Hennen WJ. 2004. Cardiovascular therapy composition including transfer factor and therapeutic methods including use of the composition. PCT/US2003/026427.
  25. Jayasuria H, Koonchanok NM, Geahlen R, McLaughlin JL, Chang CJ. 1992. Emodin, a protein tyrosine kinase inhibitor from Polygonum cuspidatum. J Nat Prod 55: 696-698. https://doi.org/10.1021/np50083a026
  26. Ko SK, Lee CR, Kim H, Baek KH, Tokuoko K, Chung SH. 2003. Inhibitory effects of stilbene derivatives from Rheum undulatum on cyclooxygenase activity. Korean J Pharmacogn 35: 171-174.
  27. Yun-Choi HS, Lee JR, Kim JH, Kim YH, Kim TH. 1987. Potential inhibitors of platelet aggregation from plant sources, IV. platelet anti-aggregatory components of Cassiae Semen. Korean J Pharmacogn 18: 203-206.
  28. Joo SM. 2005. Biological activities of antraquinones and stilbenes from Polygonum cuspidatum. MS Thesis. Sookmyung Women's University, Seoul, Korea. p 11-16.
  29. Kim HK. 2007. Phytochemical constituents of Polygonum multiflorum. MS Thesis. SungKyunkwan University, Seoul, Korea. p 6-37.
  30. Lee JP. 2001. The compounds isolated from Pleuropterus cilinervis and their antioxidative effect. MS Thesis. Chungnam National University, Daejeon, Korea. p 11-130.

Cited by

  1. Nutritional Value and Bioactive Properties of Enzymatic Hydrolysates prepared from the Livers of Oncorhynchus keta and Oncorhynchus gorbuscha (Pacific Salmon) vol.18, pp.1, 2015, https://doi.org/10.5657/FAS.2015.0013
  2. 박동관류 심방모델에서 강심효과를 나타내는 단미 한약재 검색 vol.30, pp.1, 2012, https://doi.org/10.15188/kjopp.2016.02.30.1.40
  3. Antimicrobial and Anti-Inflammatory Effects of the Ethanol Extract of Polygonum cuspidatum as a Mouthwash Component vol.16, pp.4, 2012, https://doi.org/10.1177/1934578x211005255