Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Assessing Biological Safety of the Hanwoo Serum Obtained During Slaughtering Process

도축되는 한우 혈액에서 회수한 혈청의 생물학적 안전성 분석

  • Kim, Min-Soo (Department of Veterinary Medicine, College of Veterinary Medicine, Konkuk University) ;
  • Yu, Ji-Eun (Department of Veterinary Medicine, College of Veterinary Medicine, Konkuk University) ;
  • Min, Kyung-Ho (Department of Veterinary Medicine, College of Veterinary Medicine, Konkuk University) ;
  • Kim, Ji-Hoe (School of Biotechnology, Yeungnam University) ;
  • Choi, In-Ho (School of Biotechnology, Yeungnam University) ;
  • Nahm, Sang-Soep (Department of Veterinary Medicine, College of Veterinary Medicine, Konkuk University)
  • 김민수 (건국대학교 수의과대학 수의학과) ;
  • 유지은 (건국대학교 수의과대학 수의학과) ;
  • 민경호 (건국대학교 수의과대학 수의학과) ;
  • 김지회 (영남대학교 생명공학부) ;
  • 최인호 (영남대학교 생명공학부) ;
  • 남상섭 (건국대학교 수의과대학 수의학과)
  • Received : 2011.09.20
  • Accepted : 2011.11.09
  • Published : 2012.02.29
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Abstract

Bovine serum contains various nutrients and growth factors that can be potentially used in biological experiments, drug manufacturing process and food industry. However, almost all the bovine blood has been wasted during slaughter process in Korea, thus there is a high demand for alternative uses of the wasted sera. In order to produce high quality and safe sera, it is necessary to screen zoonotic pathogens as well as other microbial contaminants to prevent any downstream contamination. The present research has been undertaken to assess biological safety of Hanwoo sera by determining microbiological contamination during slaughtering and handling processes. Serological tests have been performed to detect bacteria, mycoplasma and virus contamination in total of 52 Hanwoo sera. No sera were found to be contaminated with mycoplasma or virus, but only two sera were found to be contaminated with Bacillus thuringiensis. The present result shows that Hanwoo sera obtained from slaughtering process are biologically safe and have potentials to be developed as a biological reagent. Moreover, the methods employed in our study may provide basic standard for microbiological screening methods once wasted Hanwoo sera gain industrial values.

본 연구에서는 성체 한우의 도축과정 중에 채혈된 혈액으로부터 분리 정제한 혈청의 생물학적 안전성을 검증하기 위하여 세균, 마이코플라즈마 및 바이러스의 검출과 동정을 실시하였다. 검사를 실시한 대부분의 혈청에서 전염성 병원체가 검출되지 않았고 두 개의 혈청에서 토양 유래의 것으로 추정되는 Bacillus thuringiensis가 검출 되었다. 본 연구의 결과는 국내에서 도축되는 한우는 생물학적 안전성을 담보할 수 있는 최소한의 기본적인 안전장치를 통해 도축된다는 사실을 보여주며 한우의 도축과정에서 부산물로 얻을 수 있는 혈액을 안전한 생물학적 제제로 개발할 수 있다는 가능성을 보여주는 것으로 생각된다.

Keywords

References

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