Ocean and Polar Research

Korea Institute of Ocean Science & Technology (한국해양과학기술원)
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Picocyanobacterial Diversity and Distribution During Summer in the Northern East China Sea

하계 동중국해 북부 해역에서 초미소남세균의 다양성 및 분포 양상

  • 최동한 (한국해양연구원 해양바이오연구센터)
  • Received : 2011.11.05
  • Accepted : 2011.11.21
  • Published : 2012.03.30
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Abstract

In order to understand the spatial distribution of picocyanobacterial diversity during the summer in the northern East China Sea (ECS), their abundance and genetic diversity were investigated using flow cytometry and barcoded amplicon pyrosequencing of 16S-23S internal transcribed spacer sequences. Synechococcus abundance was high, with a range of $0.2{\times}10^5$ to $1.8{\times}10^5$ cells $ml^{-1}$. However, Prochlorococcus were found only in the eastern part of the studied area, showing a marked variation among stations [range of n.d. (not detected) to $3.3{\times}10^4$ cells $ml^{-1}$]. Eleven Synechococcus clades and five Prochlorococcus ecotypes were found to have a proportion higher than 1% among picocyanobacterial sequences, indicating high picocyanobacterial diversity in the ECS. The picocyanobacterial compositions were markedly different among stations, as well as among depths. Inflow of the Tsushima Warm Current and Changjiang diluted water was of primary importance in determining picocyanobacterial lineage diversity in the studied area. In addition, light intensity and nutrient conditions also appeared to be important in the vertical and horizontal distribution of picocyanobacterial diversity.

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