DOI QR코드

DOI QR Code

Effects of Aqueous Chlorine Dioxide and UV-C Irradiation on Decontamination and Growth of Microbes during Chilled Storage of Celery and Cherries

이산화염소수, Ultraviolet-C 또는 병합처리가 샐러리와 체리에서의 살균 및 냉장저장 중 미생물 성장에 미치는 효과

  • Song, Hyeon-Jeong (Dept. of Food Science & Technology, Chungnam National University) ;
  • Chun, Ho-Hyun (Dept. of Food Science & Technology, Chungnam National University) ;
  • Jo, Wan-Sin (Dept. of Food Science & Technology, Chungnam National University) ;
  • Song, Kyung-Bin (Dept. of Food Science & Technology, Chungnam National University)
  • Received : 2011.12.19
  • Accepted : 2012.01.28
  • Published : 2012.03.31

Abstract

The effects of a combined treatment of aqueous chlorine dioxide ($ClO_2$) and ultraviolet-C (UV-C) irradiation on microbial growth in celery and cherries were investigated. Celery and cherry samples were treated with 50 ppm $ClO_2$, UV-C at dose of 10 kJ/$m^2$, and a combination of $ClO_2$ and UV-C. The changes in the counts of Escherichia coli O157:H7 inoculated in the celery and cherries as well as those of total aerobic bacteria, yeast and molds in the celery and cherries were investigated after each treatment. After the combined treatment of aqueous $ClO_2$ and UV-C irradiation, the populations of E. coli O157:H7 in the inoculated celery and cherries were reduced by 2.8 and 3.0 log CFU/g, respectively, compared to those of the control. For the un-inoculated celery and cherries, the populations of total aerobic bacteria were reduced by 2.9 and 1.8 log CFU/g, respectively, compared to the control. In addition, the populations of yeast and molds were decreased by 1.8 and 1.2 log CFU/g, respectively. These results suggest that the combined treatment of 50 ppm $ClO_2$ and UV-C at a dose of 10 kJ/$m^2$ would be an effective technology for decontamination and improving the microbiological safety in celery and cherries during chilled storage.

신선편이 채소류 중 샐러리와 체리의 미생물학적 안전성을 확보하기 위해 50 ppm 이산화염소수와 10 kJ/$m^2$ 선량의 UV-C 조사와의 병합 처리에 따른 저장 중 미생물 수의 변화를 측정하였다. E. coli O157:H7로 접종된 샐러리와 체리의 경우, 이산화염소수와 UV-C 병합 처리구의 E. coli O157:H7 수가 대조구와 비교했을 때 각각 2.8, 3.0 log CFU/g의 감소효과를 나타냈다. 또한 접종하지 않은 샐러리와 체리의 총 호기성 균은 병합 처리로 각각 2.9, 1.8 log CFU/g이 감소되었고, 효모 및 곰팡이도 1.8, 1.2 log CFU/g의 감소효과를 나타냈다. 따라서 본 연구결과, 50 ppm 이산화염소수와 10kJ/$m^2$ UV-C 조사의 병합처리가 샐러리와 체리의 저장 중 오염될 수 있는 위해미생물 수 감소에 있어서 효과적인 살균처리 기술이라고 판단된다.

Keywords

References

  1. Chen Z, Zhu C, Zhang Y, Niu D, Du J. 2010. Effects of aqueous chlorine dioxide treatment on enzymatic browning and shelf-life of fresh-cut asparagus lettuce (Lactuca sativa L.). Postharv Biol Technol 58: 232-238. https://doi.org/10.1016/j.postharvbio.2010.06.004
  2. CDC. 2009. Centers for Disease Control and Prevention. Surveillance for foodborne disease outbreaks-United States, 2006. Morb Mortal Wkly Rep 58: 609-615.
  3. Bean NH, Griffin PM. 1990. Foodborne disease outbreak in the United States, 1973-1987: pathogens, vehicles, and trends. J Food Prot 53: 807-814.
  4. Beuchat LR. 1996. Pathogenic microorganism associated with fresh produce. J Food Prot 59: 204-216. https://doi.org/10.4315/0362-028X-59.2.204
  5. Du J, Han Y, Linton RH. 2003. Efficacy of chlorine dioxide gas in reducing Escherichia coli O157:H7 on apple surfaces. Food Microbiol 20: 583-591. https://doi.org/10.1016/S0740-0020(02)00129-6
  6. Lu Z, Yu Z, Gao X, Lu F, Zhang L. 2005. Preservation effects of gamma irradiation on fresh-cut celery. J Food Eng 67: 347-351. https://doi.org/10.1016/j.jfoodeng.2004.04.038
  7. Trinetta V, Morgan MT, Linton RH. 2010. Use of highconcentration- short-time chlorine dioxide gas treatments for the inactivation of Salmonella enterica spp. inoculated onto Roma tomatoes. Food Microbiol 27: 1009-1015. https://doi.org/10.1016/j.fm.2010.06.009
  8. Kraybill HF. 1978. Origin, classification and distribution of chemicals in drinking water with an assessment of their carcinogenic potential. In Water Chlorination. Jolly RL, ed. Ann Arbor Science, Ann Arbor, MI, USA. Vol 1, p 211-228.
  9. Moore GS, Balabrese EJ, Dinardi SR, Ruthill RW. 1978. Potential health effect of chlorine dioxide as a disinfectant in potable water supplies. Med Hypotheses 4: 481-496. https://doi.org/10.1016/0306-9877(78)90017-8
  10. Keskinen LA, Burke A, Annous BA. 2009. Efficacy of chlorine, acidic electrolyzed water and aqueous chlorine dioxide solutions to decontaminated Escherichia coli O157:H7 from lettuce leaves. Int J Food Microbiol 132: 134-140. https://doi.org/10.1016/j.ijfoodmicro.2009.04.006
  11. Mahmoud BSM, Linton RH. 2007. Inactivation kinetics of inoculated Escherichia coli O157:H7, Listeria monocytogenes and Salmonella enterica on strawberries by chlorine dioxide gas. Food Microbiol 24: 736-744. https://doi.org/10.1016/j.fm.2007.03.006
  12. Mahmoud BSM, Vaidya NA, Corvalan CM, Linton RH. 2008. Inactivation kinetics of inoculated Escherichia coli O157:H7 and Salmonella Poona on whole cantaloupe by chlorine dioxide gas. Food Microbiol 25: 857-865. https://doi.org/10.1016/j.fm.2008.05.009
  13. Karabulut OA, Ilhan K, Arslan U, Vardar C. 2009. Evaluation of the use of chlorine dioxide by fogging for decreasing postharvest decay of fig. Postharv Biol Technol 52: 313- 315. https://doi.org/10.1016/j.postharvbio.2009.01.006
  14. Schenk M, Raffellini S, Guerrero S, Blanco GA, Alzamora SM. 2011. Inactivation of Escherichia coli , Listeria innocua and Saccharomyces cerevisiae by UV-C light: study of cell injury by flow cytometry. LWT-Food Sci Technol 44: 191-198. https://doi.org/10.1016/j.lwt.2010.05.012
  15. Allende A, Artės F. 2003. UV-C radiation as a novel technique for keeping quality of fresh processed 'Lollo Rosso' lettuce. Food Res Int 36: 739-746. https://doi.org/10.1016/S0963-9969(03)00054-1
  16. Artes-Hernandez F, Robles PA, Gomez PA, Tomas-Callejas A, Artes F. 2010. Low UV-C illumination for keeping overall quality of freshcut watermelon. Postharv Biol Technol 55: 114-120. https://doi.org/10.1016/j.postharvbio.2009.09.002
  17. FDA. 2002. Ultraviolet radiation for the processing and treatment of food. Code of Federal Regulations 21, Part 179.39.
  18. Allende A, McEvoy JL, Luo Y, Artes F, Wang CY. 2006. Effectiveness of two-sided UV-C treatments in inhibiting natural microflora and extending the shelf-life of minimally processed 'Red Oak Leaf' lettuce. Food Microbiol 23: 241- 249. https://doi.org/10.1016/j.fm.2005.04.009
  19. Artes-Hernandez F, Escalona VH, Robles PA, Martinez- Hernandez GB, Artes F. 2009. Effect of UV-C radiation on quality of minimally processed spinach leaves. J Sci Food Agric 89: 414-421. https://doi.org/10.1002/jsfa.3460
  20. Obande MA, Tucker GA, Shama G. 2011. Effect of preharvest UV-C treatment of tomatoes (Aolanum lycopersicon Mill.) on ripening and pathogen resistance. Postharv Biol Technol 62: 188-192. https://doi.org/10.1016/j.postharvbio.2011.06.001
  21. Hadjok C, Mittal GS, Warriner K. 2008. Inactivation of human pathogens and spoilage bacteria on the surface and internalized within fresh produce by using a combination of ultraviolet light and hydrogen peroxide. J Appl Microbiol 104: 1014-1024. https://doi.org/10.1111/j.1365-2672.2007.03624.x
  22. Kim JY, Kim HJ, Lim GO, Jang SA, Song KB. 2010. The effects of aqueous chlorine dioxide or fumaric acid treatment combined with UV-C on postharvest quality of 'Maehyang' strawberries. Postharv Biol Technol 56: 254- 256. https://doi.org/10.1016/j.postharvbio.2010.01.013
  23. APHA. 1995. Standard methods for the examination of water and waste water. 19th ed. American Public Health Association, Washington, DC, USA. Method 4-54.
  24. Lopez-Galvez F, Gil MI, Truchado P, Selma MV, Allende A. 2010. Cross-contamination of fresh-cut lettuce after a short-term exposure during pre-washing cannot be controlled after subsequent washing with chlorine dioxide or sodium hypochlorite. Food Microbiol 27: 199-204. https://doi.org/10.1016/j.fm.2009.09.009
  25. Keskinen LA, Burke A, Annous BA. 2009. Efficacy of chlorine, acidic electrolyzed water and aqueous chlorine dioxide solutions to decontaminate Escherichia coli O157:H7 from lettuce leaves. Int J Food Microbiol 132: 134-140. https://doi.org/10.1016/j.ijfoodmicro.2009.04.006
  26. Sommers CH, Sites JE, Musgarove M. 2010. Ultraviolet light (254 nm) inactivation of pathogens on foods and stainless steel surfaces. J Food Safety 30: 470-479. https://doi.org/10.1111/j.1745-4565.2010.00220.x
  27. Guan W, Fan X, Yan R. 2012. Effects of UV-C treatment on inactivation of Escherichia coli O157:H7, microbial loads, and quality of button mushrooms. Postharv Biol Technol 64: 119-125. https://doi.org/10.1016/j.postharvbio.2011.05.017
  28. Huang TS, Xu C, Walker Ken, West P, Zhang S, Weese J. 2006. Decontamination efficacy of combined chlorine dioxide with ultrasonication on apples and lettuce. J Food Sci 71: M134-M139. https://doi.org/10.1111/j.1750-3841.2006.00015.x
  29. Selma MV, Allende A, Lopez-Galvez F, Conesa MA, Gill MI. 2008. Disinfection potential of ozone, ultraviolet-C and their combination in wash water for the fresh-cut vegetable industry. Food Microbiol 25: 809-814. https://doi.org/10.1016/j.fm.2008.04.005
  30. Chen Z, Zhu C. 2011. Combined effects of aqueous chlorine dioxide and ultrasonic treatments on postharvest storage quality of plum fruit (Prunus salicina L.). Postharv Biol Technol 61: 117-123. https://doi.org/10.1016/j.postharvbio.2011.03.006
  31. Kim HJ, Song HJ, Song KB. 2011. Effect of combined treatment of aqueous chlorine dioxide with ultraviolet-C on the quality of red chicory and pak choi during storage. J Korean Soc Food Sci Nutr 40: 245-252. https://doi.org/10.3746/jkfn.2011.40.2.245
  32. KFDA. 2011. Korean Food Standards Codex. Korea Food and Drug Administration, Seoul, Korea.
  33. Wu VCH, Kim BC. 2007. Effect of a simple chlorine dioxide method for controlling five foodborne pathogens, yeasts and molds on blueberries. Food Microbiol 24: 794-800. https://doi.org/10.1016/j.fm.2007.03.010
  34. Begum M, Hocking AD, Miskelly D. 2009. Inactivation of food spoilage fungi by ultra violet (UVC) irradiation. Int J Food Microbiol 129: 74-77. https://doi.org/10.1016/j.ijfoodmicro.2008.11.020
  35. Chun HH, Kim JY, Lee BD, Yu DJ, Song KB. 2010. Effect of UV-C irradiation on the inactivation of inoculated pathogens and quality of chicken breasts during storage. Food Control 21: 276-280. https://doi.org/10.1016/j.foodcont.2009.06.006
  36. Manzocco L, Pieve SD, Maifreni M. 2011. Impact of UV-C light on safety and quality of fresh-cut melon. Inno Food Sci Emerg Technol 12: 13-17. https://doi.org/10.1016/j.ifset.2010.11.006

Cited by

  1. Non-thermal Treatment of Postharvest Strawberry and Establishment of Its Optimal Freezing Condition vol.58, pp.1, 2015, https://doi.org/10.3839/jabc.2015.010
  2. Effects of Combined Treatment of Aqueous Chlorine Dioxide and UV-C or Electron Beam Irradiation on Microbial Growth and Quality in Chicon during Storage vol.41, pp.11, 2012, https://doi.org/10.3746/jkfn.2012.41.11.1632
  3. Effects of combined acetic acid and UV-C irradiation treatment on the microbial growth and the quality of sedum during its storage vol.21, pp.4, 2014, https://doi.org/10.11002/kjfp.2014.21.4.581
  4. Inactivation of pre-existing bacteria and foodborne pathogens on perilla leaves using a combined treatment with an organic acid and a surfactant vol.56, pp.2, 2015, https://doi.org/10.1007/s13580-015-0093-2
  5. Combined Effects of Sanitizer Mixture and Antimicrobial Ice for Improving Microbial Quality of Salted Chinese Cabbage during Low Temperature Storage vol.44, pp.11, 2015, https://doi.org/10.3746/jkfn.2015.44.11.1715