Preventive Nutrition and Food Science

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지
DOI QR코드

DOI QR Code

Antioxidant and Anti-inflammatory Activities of Butanol Extract of Melaleuca leucadendron L.

  • Surh, Jeong-Hee (Department of Food and Nutrition, College of Health and Welfare, Kangwon National University) ;
  • Yun, Jung-Mi (Department of Food and Nutrition, Kwangju Women's University)
  • Received : 2012.01.26
  • Accepted : 2012.03.02
  • Published : 2012.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

Melaleuca leucadendron L. has been used as a tranquilizing, sedating, evil-dispelling and pain-relieving agent. We examined the effects of M. leucadendron L. extracts on oxidative stress and inflammation. M. leucadendron L. was extracted with methanol (MeOH) and then fractionated with chloroform ($CHCl_3$) and butanol (BuOH). Antioxidant activity of the MeOH extract and BuOH fraction were higher than that of both ${\alpha}$-tocopherol and butyrated hydroxytoluene (BHT). Total phenol content in the extracts of M. leucadendron L., especially the BuOH fraction, well correlated with the antioxidant activity. The anti-inflammatory activity of BuOH extracts were investigated by lipopolysaccharide (LPS)-induced nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$) production, and cyclooxygenase-2 (COX-2) expression in RAW 264.7 macrophages. The BuOH fraction significantly inhibited LPS-induced NO and $PGE_2$ production. Furthermore, BuOH extract of M. leucadendron L. inhibited the expression of COX-2 and iNOS protein without an appreciable cytotoxic effect on RAW264.7 cells. The extract of M. leucadendron L. also suppressed the phosphorylation of inhibitor ${\kappa}B{\alpha}$ ($I{\kappa}B{\alpha}$) and its degradation associated with nuclear factor-${\kappa}B$ (NF-${\kappa}B$) activation. Furthermore, BuOH fraction inhibited LPS-induced NF-${\kappa}B$ transcriptional activity in a dose-dependent manner. These results suggested that M. leucadendron L. could be useful as a natural antioxidant and anti-inflammatory resource.

Keywords

References

  1. Finkel T, Holbrook NJ. 2000. Oxidants, oxidative stress and the biology of aging. Nature 408: 239-247. https://doi.org/10.1038/35041687
  2. Kabe Y, Ando Y, Hirao S, Yoshida M, Handa H. 2005. Redox regulation of inflammatory processes. Mem Inst Oswaldo Cruz 100: 5-9. https://doi.org/10.1590/S0074-02762005000900002
  3. Marnett LJ. 1992. Aspirin and the potential role of prostaglandins in colon cancer. Cancer Res 52: 5575-5589.
  4. Moncada S, Palmer RM, Higgs EA. 1991. Nitric oxide: physiology, pathophysiology, and pharmacology. Pharmacol Rev 43: 109-142.
  5. Anggard E. 1994. Nitric oxide: mediator, murderer, and medicine. Lancet 343: 1199-1206. https://doi.org/10.1016/S0140-6736(94)92405-8
  6. Surh YJ, Chun KS, Cha HH, Han SS, Keum YS, Park KK. 2001. Molecular mechanisms underlying chemopreventive activities of anti-inflammatory phytochemicals: down-regulation of COX-2 and iNOS through suppression of NF-$\kappa B$ activation. Mutat Res 480-481: 243-268. https://doi.org/10.1016/S0027-5107(01)00183-X
  7. Hibbs JB, Taintor RR, Vavrin Z. 1987. Macrophage cytotoxicity: role of L-arginine deiminase and imino nitrogen oxidation to nitrite. Science 235: 473-476. https://doi.org/10.1126/science.2432665
  8. Baeuerle PA, Baichwal VR. 1997. NF-$\kappa B$ as a frequent target for immunosuppressive and anti-inflammatory molecules. Adv Immunol 65: 111-137. https://doi.org/10.1016/S0065-2776(08)60742-7
  9. Yang TI. 1982. A list of palants in Taiwan. Natural publishing Co., Ltd., Taipei, Taiwan. p 982.
  10. Gan WS. 1965. Manual of medicinal plants in Taiwan. National Research institute of Chinese Medicine Press, Taipei, Taiwan. Vol 3, p 613.
  11. Tsuruga T, Chun YT, Ebizuka Y, Sankawa U. 1991. Biologically active constituents of Melaleuca lecadendron: inhibitors of induced histamine release from rat mast cells. Chem Pharm Bull (Tokyo) 39: 3276-3278. https://doi.org/10.1248/cpb.39.3276
  12. Gutfinger T. 1981. Polyphenols in olive oil. J Am Oil Chem Soc 58: 966-968. https://doi.org/10.1007/BF02659771
  13. Shirwaikar A, Somashekar AP. 2003. Anti-inflammatory activity and free radical scavenging studies of Aristolochia bracteolate Lam. Indian J Pharm Sci 65:68.
  14. Kirby AJ, Schmidt RJ. 1997. The antioxidant activity of Chinese herbs for eczema and placebo herbs-I. J Ethnoparmacol 56: 103-108. https://doi.org/10.1016/S0378-8741(97)01510-9
  15. Mitsuda H, Yasumoto K, Iwama K. 1996. Antioxidative action of indole compounds during the autooxidation of linoleic acid. Eiyo Shokuryo 19: 210-214.
  16. Tubaro A, Florio C, Luxich E, Vertua R, Loggia RD, Yasumoto T. 1996. Suitability of the MTT-based cytotoxicity assay to detect okadaic acid contamination of mussels. Toxicon 34: 965-974. https://doi.org/10.1016/0041-0101(96)00073-6
  17. Schmidt HHHW, Kelm M. 1996. Determination of nitrite and nitrate by the Griess reaction. In Methods in Nitric Oxide Research. John Wiley & Sons Ltd., London, UK. p 491-497.
  18. Kunyanga CN, Imungi JK, Okoth MW, Biesaiski HK, Vadivel V. 2012. Total phenolic content, antioxidant and antidiabetic properties of methanolic extract of raw and traditionally processed Kenyan indigenous food ingredients. LWT-Food Sci Technol 45: 269-276. https://doi.org/10.1016/j.lwt.2011.08.006
  19. Lee JY, Jang YW, Kang HS, Moon H, Sim SS, Kim CJ. 2006. Anti-inflammatory action of phenolic compounds from Gastrodia elata root. Arch Pharm Res 29: 849-858. https://doi.org/10.1007/BF02973905
  20. Kim MY, Seguin P, Ahn JK, Kim JJ, Chun SC, Kim EH, Seo SH, Kang EY, Kim SL, Park YJ, Ro HM, Chung IM. 2008. Phenolic compound concentration and antioxidant activities of edible and medicinal mushrooms from Korea. J Agric Food Chem 56: 7265-7270. https://doi.org/10.1021/jf8008553
  21. Kundu JK, Na HK, Surh YJ. 2009 Ginger-derived phenolic substances with cancer preventive and therapeutic potential. Forum Nutr 61: 182-192. https://doi.org/10.1159/000212750
  22. Kris-Etherton PM, Hecker KD, Bonanome A, Coval SM, Binkoski AE, Hilpert KF, Griel AE, Etherton TD. 2002. Bioactive compounds in foods: their role in the prevention of cardiovascular disease and cancer. Am J Med 113: 71S- 88S. https://doi.org/10.1016/S0002-9343(01)00995-0
  23. Rice-Evans CA, Miller NJ. 1996. Antioxidant activities of flavonoids as bioactive components of food. Biochem Soc Trans 24: 790-795. https://doi.org/10.1042/bst0240790
  24. Yin MJ, Yamamoto Y, Graynor RB. 1998. The anti-inflammatory agents aspirin and salicylate inhibit the activity of $I \kappa B$-$\beta$. Nature 396: 77-80. https://doi.org/10.1038/23948

Cited by

  1. Anti-inflammatory Effect of Mangosteen (Garcinia mangostanaL.) Peel Extract and its Compounds in LPS-induced RAW264.7 Cells vol.22, pp.3, 2016, https://doi.org/10.20307/nps.2016.22.3.147
  2. Anti-oxidative and Anti-inflammatory Effect of 7 Herbal Extracts and Methods of Herbal Formula Compositioning vol.22, pp.2, 2014, https://doi.org/10.14374/HFS.2014.22.2.087
  3. Anti-inflammatory Activity and Phytochemical Profile of Galinsoga Parviflora Cav. vol.23, pp.9, 2018, https://doi.org/10.3390/molecules23092133
  4. Antioxidant, cytotoxicity and phytochemical analysis of Larinus maculates F. cocoon aqueous extract against lung cancer vol.1879, pp.2, 2012, https://doi.org/10.1088/1742-6596/1879/2/022025
  5. Biochemical Aspect, Antimicrobial and Antioxidant Activities of Melaleuca and Syzygium Species (Myrtaceae) Grown in Egypt vol.1879, pp.2, 2012, https://doi.org/10.1088/1742-6596/1879/2/022062