Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
권호 표지
DOI QR코드

DOI QR Code

New Method for Simultaneous Quantification of 12 Ginsenosides in Red Ginseng Powder and Extract: In-house Method Validation

  • In, Gyo (Analysis Research Team, R&D Headquarters, Korea Ginseng Corporation) ;
  • Ahn, Nam-Geun (Analysis Research Team, R&D Headquarters, Korea Ginseng Corporation) ;
  • Bae, Bong-Seok (Analysis Research Team, R&D Headquarters, Korea Ginseng Corporation) ;
  • Han, Sung-Tai (Analysis Research Team, R&D Headquarters, Korea Ginseng Corporation) ;
  • Noh, Kil-Bong (Analysis Research Team, R&D Headquarters, Korea Ginseng Corporation) ;
  • Kim, Cheon-Suk (Analysis Research Team, R&D Headquarters, Korea Ginseng Corporation)
  • Received : 2011.01.24
  • Accepted : 2012.02.27
  • Published : 2012.04.15
Download PDF
⟨ Previous Next ⟩

Abstract

For quality control of components in Korean red ginseng powder and extract, a new method for simultaneous quantification of 12 ginsenosides ($Rg_1$, Re, Rf, $Rh_1$, $Rg_2$[S], $Rg_2$[R], $Rb_1$, Rc, $Rb_2$, Rd, $Rg_3$[S], and $Rg_3$[R]) was studied. Compared to the official method for quantification of marker substances (ginsenosides $Rg_1$ and $Rb_1$), the proposed methods were guaranteed by in-house method validation. Several criteria such as linearity, specificity, precision and accuracy were evaluated. For red ginseng powder, recovery (averaging 95% to 105%) was calculated, and analysis of variance was carried out to estimate the relative standard deviation (0.20% to 2.12%). For red ginseng extract, the average recovery rate was 90% to 99% and the relative standard deviation was 0.39% to 2.40%. These results indicate that the proposed method could be used in the laboratory for determination of 12 ginsenosides in red ginseng powder and extract. In addition, this method was found to be suitable for quality control of ginseng products and potentially offer time and cost benefits.

Keywords

References

  1. Christensen LP. Ginsenosides chemistry, biosynthesis, analysis, and potential health effects. Adv Food Nutr Res 2009;55:1-99.
  2. Park CK, Kwak YS, Hwang MS, Kim SC, Do JH. Trends and prospect of ginseng products in market health functional food. Food Sci Ind 2007;40:30-45.
  3. Korean Agency for Technology and Standards. Ginseng and ginseng products - determination of ginsenoside ($Rg_1$, $Rb_1$) contents - method by high performance liquid chromatography. KSH2153. 2008 Mar 26.
  4. Korea Food & Drug Administration. Health functional food code. Seoul: Korea Food & Drug Administration, 2010.
  5. Fuzzati N. Analysis methods of ginsenosides. J Chromatogr B Analyt Technol Biomed Life Sci 2004;812:119-133. https://doi.org/10.1016/S1570-0232(04)00645-2
  6. Yap KY, Chan SY, Lim CS. Infrared-based protocol for the identification and categorization of ginseng and its products. Food Res Int 2007;40:643-652. https://doi.org/10.1016/j.foodres.2006.11.009
  7. Ji HY, Lee HW, Kim HK, Kim HH, Chang SG, Sohn DH, Kim J, Lee HS. Simultaneous determination of ginsenoside $Rb_1$ and $Rg_1$ in human plasma by liquid chromatography-mass spectrometry. J Pharm Biomed Anal 2004;35:207-212. https://doi.org/10.1016/j.jpba.2003.12.020
  8. Dan M, Su M, Gao X, Zhao T, Zhao A, Xie G, Qiu Y, Zhou M, Liu Z, Jia W. Metabolite profiling of Panax notoginseng using UPLC-ESI-MS. Phytochemistry 2008;69:2237-2244. https://doi.org/10.1016/j.phytochem.2008.04.015
  9. Shi Y, Sun C, Zheng B, Li Y, Wang Y. Simultaneous determination of nine ginsenosides in functional foods by high performance liquid chromatography with diode array detector detection. Food Chem 2010;123:1322-1327. https://doi.org/10.1016/j.foodchem.2010.06.014
  10. Kim SN, Ha YW, Shin H, Son SH, Wu SJ, Kim YS. Simultaneous quantification of 14 ginsenosides in Panax ginseng C.A. Meyer (Korean red ginseng) by HPLCELSD and its application to quality control. J Pharm Biomed Anal 2007;45:164-170. https://doi.org/10.1016/j.jpba.2007.05.001
  11. Taverniers I, Loose MD, Bockstaele EV. Trends in quality in the analytical laboratory. II. Analytical method validation and quality assurance. TrAC Trends Anal Chem 2004;23:535-552. https://doi.org/10.1016/j.trac.2004.04.001
  12. Ermer J. Validation in pharmaceutical analysis. Part I: an integrated approach. J Pharm Biomed Anal 2001;24:755-767. https://doi.org/10.1016/S0731-7085(00)00530-6

Cited by

  1. vol.2013, pp.1466-1861, 2013, https://doi.org/10.1155/2013/761506
  2. A formulated red ginseng extract upregulates CHOP and increases TRAIL-mediated cytotoxicity in human hepatocellular carcinoma cells vol.43, pp.2, 2013, https://doi.org/10.3892/ijo.2013.1964
  3. Evaluation of Antibacterial and Anti-inflammatory Activities of Less Polar Ginsenosides Produced from Polar Ginsenosides by Heat-transformation vol.61, pp.50, 2013, https://doi.org/10.1021/jf404461q
  4. Comparative Study on Immuno-Enhancing Effects of Red Ginseng Fractions vol.43, pp.11, 2014, https://doi.org/10.3746/jkfn.2014.43.11.1665
  5. Identification and Characterization of a Ginsenoside-Transforming β-Glucosidase from Pseudonocardia sp. Gsoil 1536 and Its Application for Enhanced Production of Minor Ginsenoside Rg2(S) vol.9, pp.6, 2014, https://doi.org/10.1371/journal.pone.0096914
  6. Physicochemical Properties and Fibrinolytic Activity of Ginseng Powder Fermented with <i>Bacillus subtilis</i> Isolated from <i>Cheonggukjang</i> vol.08, pp.08, 2017, https://doi.org/10.4236/ajps.2017.88126
  7. Simultaneous determination of 30 ginsenosides in Panax ginseng preparations using ultra performance liquid chromatography vol.37, pp.4, 2013, https://doi.org/10.5142/jgr.2013.37.457
  8. Quantitative Analysis of Ginsenosides Using Relative Response Factors from Ginsenoside Rf vol.16, pp.2, 2012, https://doi.org/10.20402/ajbc.2017.0186
  9. Simultaneous Quantification of 13 Ginsenosides by LC-MS/MS and its Application in Diverse Ginseng Extracts vol.9, pp.2, 2012, https://doi.org/10.5478/msl.2018.9.2.41
  10. 인삼의 열처리 과정 중 생성되는 3종의 수산화진세노사이드에 대한 연구 vol.51, pp.4, 2012, https://doi.org/10.22889/kjp.2020.51.4.255
  11. Quality Distinguish of Red Ginseng from Different Origins by HPLC-ELSD/PDA Combined with HPSEC-MALLS-RID, Focus on the Sugar-Markers vol.8, pp.11, 2012, https://doi.org/10.3390/separations8110198