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A Study on the Depigmenting Effect of Carthamus tinctorius Seed, Cyperus rotundus and Schizonepeta tenuifolia Extracts

홍화자, 향부자, 형개 추출물의 미백효과에 관한 연구

  • Hwang, Eun-Young (Korea Promotion Institute for Oriental Medicine Industry) ;
  • Kim, Dong-Hee (Korea Promotion Institute for Oriental Medicine Industry) ;
  • Hwang, Jo-Young (Korea Promotion Institute for Oriental Medicine Industry) ;
  • Kim, Hui-Jeong (Korea Promotion Institute for Oriental Medicine Industry) ;
  • Park, Tae-Soon (Korea Promotion Institute for Oriental Medicine Industry) ;
  • Lee, In-Sun (Department of Food and Technology, Keimyung University) ;
  • Son, Jun-Ho (Korea Promotion Institute for Oriental Medicine Industry)
  • Received : 2011.07.19
  • Accepted : 2011.11.29
  • Published : 2012.02.29

Abstract

The objective of the present study was to evaluate the skin depigmentation effect of the extracts of three herbs, Carthamus tinctorius seed, Cyperus rotundus and Schizonepeta tenuifolia. Their effects on tyrosinase and melanin synthesis inhibitory action were assessed. We found that the C. tinctorius seed ethanol extracts reduced the tyrosinase activity and melanin formation of B16F10 melanoma cells. The C. tinctorius seed suppressed the expression in microphthalmia associated transcription factor (MITF), tyrosinase, tyrosinase related protein 1 (TRP-1), and tyrosinase related protein 2 (TRP-2) in B16F10 melanoma cells. These results show that C. tinctorius seed inhibited melanogenesis on the B16F10 melanoma cell. The underlying mechanism of C. tinctorius seed whitening activity may be the inhibition of tyrisinase, MITF, tyrosinase, TRP-1, and TRP-2 expression. The results suggested that C. tinctorius seed has considerable potential as a natural functional ingredient with a depigmentation effect.

본 연구에서는 3가지 홍화자, 향부자, 형개 추출물의 미백효과를 연구하였다. 멜라닌 생성 첫 단계인 tyrosinase 억제활성과 멜라닌 생성 억제효과를 측정한 결과, 홍화자 에탄올 추출물이 tyrosinase 활성과 B16F10 melanoma 세포의 멜라닌 생성 억제하였다. 그 결과, 홍화자 추출물은 B16F10 melanoma 세포에서 melanogenesis 따른 tyrosinase 형성 억제에 따른 멜라닌 합성 관련 인자 MITF, tyrosinase, TRP-1, TRP-2 의 발현을 억제함에 따라 홍화자 추출물의 미백효과를 확인하였다. 따라서 홍화자는 미백효과를 가진 천연 기능성 재료로서 가능성이 매우 높은 것으로 판단된다.

Keywords

References

  1. Yu YG, Jeong MS, Choe JY, Kim JY. A study on whitening effect of Ephedra sinica extracts. Korean J. Design Cult. Soc. 31: 153-159 (2005)
  2. Jeong MH, Kim SS, Kim JS, Lee HJ, Chio GP, Lee HY. Jour. Skin whitening and skin immune activities of different parts of Acer mono and Acer okamotoanum. Korean For. Soc. 99: 470- 478 (2005)
  3. Chen JS, Wei C, Marxhall MR. Inhibition mechanism of koji acid on polyphenol oxidase. J. Agr. Food Chem. 58: 79-110 (1991)
  4. Urabe K, Aroca P, Tsukamoto K, Mascagna D, Paulumbo A, Prota G, Hering VJ. The inherent cytotoxicty of melanin precursors. Biochim. Biophys. Acta 1221: 272-278 (1994) https://doi.org/10.1016/0167-4889(94)90250-X
  5. Hearing VJ, Tsukamoto K. Enzymatic control of pigmentation in mammals. FASEB J. 5: 2902-2909 (1991)
  6. Jimenez-Cervantes C, Solano F, Kobayashi T, Urabe K, hearing V, Lezano J, Garcia-Gorreni JC. A new enzymatic function in the melanogenic pathway. J. Biol. Chem. 269: 17993-18000 (1994)
  7. Parvez S, Malik K, Ah KS, Kim HY. Probiotics and their fermented food products are beneficial for health. J. Appl. Microbiol. 100: 1171-1185 (2006) https://doi.org/10.1111/j.1365-2672.2006.02963.x
  8. Busca R, Ballotti R. Cyclic AMP a key messenger in the regulation of skin pigmentation. Pigm. Cell Res. 13: 60-69 (2000) https://doi.org/10.1034/j.1600-0749.2000.130203.x
  9. Sassone CP. Coupling gene expression to cAMP signalling: Role of CREB and CREM. Int. J. Biochem. Cell B. 30: 27-38 (1998) https://doi.org/10.1016/S1357-2725(97)00093-9
  10. Saha B, Singh SK, Sarkar C, Bera R, Ratha J, Tobin DJ, Bhadra R. Activation of the Mitf promoter by lipid-stimulated activation of p38-stress signalling to CREB. Pigm. Cell Res. 29: 595-605 (2006)
  11. Maeda K, Fukuda M. Arbutin: Mechanism of its depigmenting action in human melanocyte culture. J. Phamacol. Exp. Ther. 276: 765-769 (1996)
  12. Battaini GE, Monzani L, Casella L, Santagostini R, Pagliarin. Inhibition of the catecholase activity of biomimetic dinuclear copper complexes by kojic acid. J. Biol. Inorg. Chem. 5: 262-268 (2000) https://doi.org/10.1007/s007750050370
  13. Chun HJ, Chli WH, Baek SH, Woo WH. Effect of quercetin on melanogenesis in melan-a melanocyte cells. Korean J. Pharmacogn. 33: 245-251 (2002)
  14. Curto EV, Kwong C, Hermersdorfer H, Glatt C, Santis V, Virador VJ and Dooley TP. Inhibitions of mammalian melanocytes tyrosinase: In vitro comparisons of alkyl esters of gentiic acid with other putative inhibitors. Biochem. Pharmacol. 57: 663-672 (1999) https://doi.org/10.1016/S0006-2952(98)00340-2
  15. Seo BI, Lee ES, Park JH, Kim SC, Byun BH, Choi HY. A efficacy of Korean and Chinese Carthami Semen on analgesic effect, anti-inflammation and arthritis. Korean J. Herbol. 16: 1-10 (2001)
  16. Hur TY, Yun MY, Cho EH, Lee OJ, Kim KS, Cho NG. Clinical study on dffect of carthmi-Flos Herbal acupuncture therapy on osteoarthritis in knee joint. Korean J. Acupunct. Moxibustion Soc. 19: 189-200 (2002)
  17. Seung KR, Jung KH. Effect of Carthamus tinctorius L. semen on endotoxin-induced thrombosis in rats. J. Pharmacol. 9: 258-262 (2001)
  18. Jung KW, Jeong CS, Jeong JS. Effect of subfractions of Carthamus tinctorius L. semen on the lipid peroxidation and oxygen free radical scavenging enzyme activities in CCI4-induced hepatotoxic rats. J. Food Hyg. Safety 14: 179-185 (1999)
  19. Park JY. Comparative studies on the physiological activities of processed and unprocessed cyperi rhizomata. PhD thesis, Sookmyung Women's University, Seoul, Korea (1996)
  20. Hikino H. Apta K, Takemoto T. Structure and absolute configuration of Cyperotundone. Chem Pharm. Bull. 14: 890-896 (1966) https://doi.org/10.1248/cpb.14.890
  21. Gupta MB, Palit TK, Singh N, Bhargava KP. Pharmacological studies to isolate the active constituents from Cyperus rotundus possing anti-inflammatory, anti-pyretic, and analgesic activities. Indian J. Med. Res 59: 76-82 (1970)
  22. Moon JY. Distinction and phylogenetic analysies of Citri unshiu semen, Schizonepetae spike and Prunella spike on basis of DNA sequences. PhD thesis, Kyunghee University, Seoul, Korea (2011)
  23. Yagi AT, Kanbara T, Morinobu N. The effect of tyrosinase inhibition for aloe. Planta Med. 3981: 517-519 (1986)
  24. Michikawa M, Lim KT, McLarnon JG, Kim SU. Oxygen radicalinduced neurotoxicity in spinal cord neuron cultures. J. Neurosci. Res. 37: 62-70 (1994) https://doi.org/10.1002/jnr.490370109
  25. Carmichael J, Defraff WG, Gazdar AF, Minna JD, Mitchell JB. Evaluation of a tetrazolium based semiautomated colorimetric assay: Assessment of chemosensitivuty tesing. Cancer Res. 47: 936-942 (1987)
  26. Hosoi JE, Abe T, Suda T, Kuroki. Regulation of melanin synthesis of B16 melanoma cells by 1 alpha, 25-dihydroxyvitamin D3 and retinoic acid. J. Cancer. Res. 45: 1474-1478 (1985)
  27. Cho YJ. Study om the tyrosinase activity inhibition and free radical scavenging activity of natural materials. MS thesis, Sookmyung Womans University, Seoul, Korea. (2004)
  28. Lee NH, Yang HC, Bu HJ, Jung DS, Lee SJ, Riu KZ. Screening of the tyrosinase inhibition and hyaluronidase inhibition activities and radical scavenging effects using plants in Jeju. Korean J. Pharmacogn. 32:175-180 (2001)
  29. Carmichael J, Degraff WG, Gadzar AF. Evaluation of a tetrazolium based semiautomated colorimetric assay: Assessment of chemosensitivity testing. Cancer Res. 477: 936-942 (1987)
  30. Sapkota K, Park SE, Kim JE, Kim S, Choi HS, Chun HS, Kim SJ. Antioxidant and antimelanogenic properties of chestnut flower extract. Biosci. Biotech. Bioch. 74: 1527-1533 (2010) https://doi.org/10.1271/bbb.100058
  31. Ha JH, Kwon MC, Kim SS, Jeong MH, Hwang B, Lee HY. Enhancement of skin-whitening and UV-protective effects of Centella asiatica L. Urban by Utrasonification Pross. Korean J. Medicinal Crop Sci. 18: 79-85 (2010)
  32. Han GS, Kim DS, Woo WH, Mun YJ. Inhibitory effect of fructus Ligustri Lucidi on tyrosinase and MITF expressions. Korean J. Orient. Physiol. Pathol. 24: 296-301 (2010)
  33. Oh HC, Lim KS, Hwang CY, Youn IH, Kim NK. A Study on the melanin synthesis inhibition and whitening effect of bombysis corpus. Korean J. Oriental Med. 20: 1-13 (2007)
  34. Park SH, Lee BY, Han CS, Kim JG, Kim KT, Kim KH, Kim YH. Whitening effect of Angelica keiskei koidzumi extract by inhibition of endothelin-1 production and melanogenesis. Korean J. Soc. Cosmet. Sci. 34: 101-107 (2008)
  35. Kim TH, You JK, Kim JM, Baek JM, Kim HS, Park JH, Choe M. Antioxidant and whitening effects of Sorbus commixta hecl cortex Extract. Korean J. Soc. Food Sci. Nutr. 39: 1418-1424 (2010) https://doi.org/10.3746/jkfn.2010.39.10.1418

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