Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Molecular Characterization and Expression Pattern of Gene IGFBP-5 in the Cashmere Goat (Capra hircus)

  • Wang, X.J. (College of Life Science, Inner Mongolia University) ;
  • Shi, J.J. (College of Life Science, Inner Mongolia University) ;
  • Yang, J.F. (College of Life Science, Inner Mongolia University) ;
  • Liang, Y. (College of Life Science, Inner Mongolia University) ;
  • Wang, Y.F. (College of Life Science, Inner Mongolia University) ;
  • Wu, M.L. (College of Life Science, Inner Mongolia University) ;
  • Li, S.Y. (College of Life Science, Inner Mongolia University) ;
  • Guo, X.D. (College of Life Science, Inner Mongolia University) ;
  • Wang, Z.G. (College of Life Science, Inner Mongolia University) ;
  • Liu, D.J. (College of Life Science, Inner Mongolia University)
  • Received : 2011.08.19
  • Accepted : 2011.12.01
  • Published : 2012.05.01
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Abstract

Insulin-like growth factor-binding protein-5 (IGFBP-5) is one of the six members of IGFBP family, important for cell growth, apoptosis and other IGF-stimulated signaling pathways. In order to explore the significance of IGFBP-5 in cells of the Inner Mongolian Cashmere goat (Capra hircus), IGFBP-5 gene complementary DNA (cDNA) was amplified by reverse transcription polymerase chain reaction (RT-PCR) from the animal's fetal fibroblasts and tissue-specific expression analysis was performed by semi-quantitative RT-PCR. The gene is 816 base pairs (bp) in length and includes the complete open reading frame, encoding 271 amino acids (GenBank accession number JF720883). The full cDNA nucleotide sequence has a 99% identity with sheep, 98% with cattle and 95% with human. The amino acids sequence shares identity with 99%, 99% and 99%, respectively. The bioinformatics analysis showed that IGFBP-5 has an insulin growth factor-binding protein homologues (IB) domain and a thyroglobulin type-1 (TY) domain, four protein kinase C phosphorylation sites, five casein kinase II phosphorylation sites, three prenyl group binding sites (CaaX box). The IGFBP-5 gene was expressed in all the tested tissues including testis, brain, liver, lung, mammary gland, spleen, and kidney, suggesting that IGFBP-5 plays an important role in goat cells.

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References

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