Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
권호 표지

The Effects of 3-Isobutyl-1-methylxanthine (IBMX) on Nuclear and Cytoplasmic Maturation of Porcine Oocytes In Vitro

  • Kwak, Seong-Sung (Laboratory of Veterinary Embryology and Biotechnology, Department of Veterinary Medicine, College of Veterinary Medicine, Chungbuk National University) ;
  • Jang, Seung-Hoon (Laboratory of Veterinary Embryology and Biotechnology, Department of Veterinary Medicine, College of Veterinary Medicine, Chungbuk National University) ;
  • Jeong, Se-Heon (Laboratory of Veterinary Embryology and Biotechnology, Department of Veterinary Medicine, College of Veterinary Medicine, Chungbuk National University) ;
  • Jeon, Yubyeol (Laboratory of Veterinary Embryology and Biotechnology, Department of Veterinary Medicine, College of Veterinary Medicine, Chungbuk National University) ;
  • Biswas, Dibyendu (Laboratory of Veterinary Embryology and Biotechnology, Department of Veterinary Medicine, College of Veterinary Medicine, Chungbuk National University) ;
  • Hyun, Sang-Hwan (Laboratory of Veterinary Embryology and Biotechnology, Department of Veterinary Medicine, College of Veterinary Medicine, Chungbuk National University)
  • Received : 2012.08.19
  • Accepted : 2012.09.05
  • Published : 2012.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

The 3-isobutyl-1-methylxanthine (IBMX) is non-selective phosphodiesterase and is able to prevent resumption of meiosis by maintaining elevated cyclic AMP (cAMP) concentrations in the oocyte. The present study was conducted to analyze: (1) nuclear maturation (examined by the Hoechst staining), (2) whether cytoplasmic maturation (examined by the intracellular glutathione (GSH) concentration) of porcine oocytes is improved during meiotic arrest after prematuration (22 h) with IBMX. Before in vitro maturation (IVM), oocytes were treated with 1 mM IBMX for 22 h. After 22 h of pre-maturation, the higher rate of IBMX treated group oocytes were arrested at the germinal vesicle (GV) stage (42.3%) than control IVM oocytes (10.1%). It appears that the effect of IBMX on the resumption of meiosis has shown clearly. In the end of IVM, the reversibility of the IBMX effect on the nuclear maturation has been corroborated in this study by the high proportions of MII stage oocytes (72.5%) reached after 44 h of IVM following the 22 h of inhibition. However, intracellular GSH concentrations were lower in the oocytes treated with IBMX than the control oocytes (6.78 and 12.94 pmol/oocyte, respectively). These results demonstrate that cytoplasmic maturation in porcine oocytes pre-treated with IBMX for 22 h did not equal that of control oocytes in the current IVM system. These results indicate that pre-maturation with IBMX for 22 h may not be beneficial in porcine IVM system.

Keywords

References

  1. Aberdam E, Hanski E and Dekel N. 1987. Maintenance of meiotic arrest in isolated rat oocytes by the invasive adenylate cyclase of Bordetella pertussis. Biol. Reprod. 36:530-535. https://doi.org/10.1095/biolreprod36.3.530
  2. Abeydeera L, Wang W, Cantley T, Prather R and Day B. 1998. Presence of [beta]-mercaptoethanol can increase the glutathione content of pig oocytes matured in vitro and the rate of blastocyst development after in vitro fertilization. Theriogenology 50:747-756. https://doi.org/10.1016/S0093-691X(98)00180-0
  3. Aktas H, Wheeler M, Rosenkrans C, First N and Leibfried-Rutledge M. 1995. Maintenance of bovine oocytes in prophase of meiosis I by high [cAMP] i. J. Reprod. Fertil. 105:227-235 https://doi.org/10.1530/jrf.0.1050227
  4. Anderiesz C, Fong CY, Bongso A and Trounson A. 2000. Regulation of human and mouse oocyte maturation in vitro with 6-dimethylaminopurine. Hum. Reprod. 15: 379-388. https://doi.org/10.1093/humrep/15.2.379
  5. Baker MA, Cerniglia GJ and Zaman A. 1990. Microtiter plate assay for the measurement of glutathione and glutathione disulfide in large numbers of biological samples. Anal. Biochem. 190: 360-365. https://doi.org/10.1016/0003-2697(90)90208-Q
  6. Barretto L, Caiado Castro V, Garcia J and Mingoti G. 2007. Role of roscovitine and IBMX on kinetics of nuclear and cytoplasmic maturation of bovine oocytes in vitro. Anim. Reprod. Sci. 99: 202-207. https://doi.org/10.1016/j.anireprosci.2006.06.001
  7. Cho WK, Stern S and Biggers JD. 1974. Inhibitory effect of dibutyryl cAMP on mouse oocyte maturation in vitro. J. Exp. Zoo. 187: 383-386. https://doi.org/10.1002/jez.1401870307
  8. Dekel N, Lawrence TS, Gilula NB and Beers WH. 1981. Modulation of cell-to-cell communication in the cumulus-oocyte complex and the regulation of oocyte maturation by LH. Dev. Biol. 86:356-362. https://doi.org/10.1016/0012-1606(81)90193-7
  9. Eppig JJ, Schultz RM, O'Brien M and Chesnel F. 1994. Relationship between the developmental programs controlling nuclear and cytoplasmic maturation of mouse oocytes. Dev. Biol. 164:1-9. https://doi.org/10.1006/dbio.1994.1175
  10. Faerge I, Mayes M, Hyttel P and Sirard M. 2001. Nuclear ultrastructure in bovine oocytes after inhibition of meiosis by chemical and biological inhibitors. Mol. Reprod. Dev. 59:459-467. https://doi.org/10.1002/mrd.1053
  11. Guixue Z, Luciano A, Coenen K, Gandolfi F and Sirard M. 2001. The influence of cAMP before or during bovine oocyte maturation on embryonic developmental competence. Theriogenology 55:1733-1743. https://doi.org/10.1016/S0093-691X(01)00516-7
  12. Kaedei Y, Fujiwara A, Ito A, Tanihara F, Morita Y, Hanatate K, Viet VL, Namula Z and Otoi T. 2010. Effect of roscovitine pretreatment on the meiotic maturation of bovine oocytes and their subsequent development after somatic cell nuclear transfer. J. Anim. Vet. Adv. 9:2848-2853. https://doi.org/10.3923/javaa.2010.2848.2853
  13. Le Beux G, Richard FJ and Sirard MA. 2003. Effect of cycloheximide, 6-DMAP, roscovitine and butyrolactone I on resumption of meiosis in porcine oocytes. Theriogenology 60:1049-1058. https://doi.org/10.1016/S0093-691X(03)00124-9
  14. Maller J, Butcher FR and Krebs EG. 1979. Early effect of progesterone on levels of cyclic adenosine 3': 5'-monophosphate in Xenopus oocytes. J. Biol. Chem. 254:579-582.
  15. Marchal R, Caillaud M, Martoriati A, Gerard N, Mermillod P and Goudet G. 2003. Effect of growth hormone (GH) on in vitro nuclear and cytoplasmic oocyte maturation, cumulus expansion, hyaluronan synthases, and connexins 32 and 43 expression, and GH receptor messenger RNA expression in equine and porcine species. Biol. Reprod. 69:1013-1022. https://doi.org/10.1095/biolreprod.103.015602
  16. Meijer L, Dostmann W, Genieser H, Butt E and Jastorff B. 1989. Starfish oocyte maturation: evidence for a cyclic AMPdependent inhibitory pathway. Dev. Biol. 133:58-66. https://doi.org/10.1016/0012-1606(89)90296-0
  17. Nagai T. 2001. The improvement of in vitro maturation systems for bovine and porcine oocytes. Theriogenology 55: 1291-1301. https://doi.org/10.1016/S0093-691X(01)00483-6
  18. Ponderato N, Crotti G, Turini P, Duchi R, Galli C and Lazzari G. 2002. Embryonic and foetal development of bovine oocytes treated with a combination of butyrolactone I and roscovitine in an enriched medium prior to IVM and IVF. Mol. Reprod. Dev. 62: 513-518. https://doi.org/10.1002/mrd.10134
  19. Racowsky C. 1984. Effect of forskolin on the spontaneous maturation and cyclic AMP content of rat oocyte-cumulus complexes. J. Reprod. Fertil. 72: 107-116. https://doi.org/10.1530/jrf.0.0720107
  20. Schultz RM, Montgomery RR and Belanoff JR. 1983. Regulation of mouse oocyte meiotic maturation: implication of a decrease in oocyte cAMP and protein dephosphorylation in commitment to resume meiosis. Dev. Biol. 97: 264-273. https://doi.org/10.1016/0012-1606(83)90085-4
  21. Shimada M, Nishibori M, Isob N, Kawano N and Terada T. 2003. Luteinizing hormone receptor formation in cumulus cells surrounding porcine oocytes and its role during meiotic maturation of porcine oocytes. Biol. Reprod. 68:1142-1149. https://doi.org/10.1095/biolreprod.102.010082
  22. Sirard M. 2001. Resumption of meiosis: mechanism involved in meiotic progression and its relation with developmental competence. Theriogenology 55:1241-1254. https://doi.org/10.1016/S0093-691X(01)00480-0
  23. Thomas RE, Armstrong DT and Gilchrist RB. 2002. Differential effects of specific phosphodiesterase isoenzyme inhibitors on bovine oocyte meiotic maturation. Dev. Biol. 244: 215-225. https://doi.org/10.1006/dbio.2002.0609
  24. Tsafriri A and Dekel N. 1994. Molecular mechanisms in ovulation. Molecular Biology of the Female Reproductive System. New York: Academic Press 207-258.
  25. Vivarelli E, Conti M, De Felici M and Siracusa G. 1983. Meiotic resumption and intracellular cAMP levels in mouse oocytes treated with compounds which act on cAMP metabolism. Cell. Diff. 12:271-276. https://doi.org/10.1016/0045-6039(83)90023-4
  26. Wang W, Abeydeera L, Cantley T and Day B. 1997. Effects of oocyte maturation media on development of pig embryos produced by in vitro fertilization. Reproduction 111:101-108. https://doi.org/10.1530/jrf.0.1110101
  27. Wang WH, Abeydeera LR, Han YM, Prather RS and Day BN. 1999. Morphologic evaluation and actin filament distribution in porcine embryos produced in vitro and in vivo. Biol. Reprod. 60: 1020-1028. https://doi.org/10.1095/biolreprod60.4.1020
  28. Wu GM, Sun QY, Mao J, Lai L, McCauley TC, Park KW, Prather RS, Didion BA and Day BN. 2002. High developmental competence of pig oocytes after meiotic inhibition with a specific M-phase promoting factor kinase inhibitor, butyrolactone I. Biol. Reprod. 67:170-177. https://doi.org/10.1095/biolreprod67.1.170