Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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Identification of SNPs in TG and EDG1 genes and their relationships with carcass traits in Korean cattle (Hanwoo)

한우에서 TG와 EDG1 유전자의 단일염기다형 확인 및 도체형질과의 연관성 분석

  • Cahyadi, Muhammad (Department of Animal Science and Biotechnology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Maharani, Dyah (Department of Animal Science and Biotechnology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Ryoo, Seung Heui (Livestock Research Institute, Government of Chungcheongnam-Do) ;
  • Lee, Seung Hwan (Hanwoo Experiment Station, National Institute of Animal Science, RDA) ;
  • Lee, Jun Heon (Department of Animal Science and Biotechnology, College of Agriculture and Life Sciences, Chungnam National University)
  • 카야디 (충남대학교 농업생명과학대학 동물자원생명과학과) ;
  • 디아 마하라니 (충남대학교 농업생명과학대학 동물자원생명과학과) ;
  • 유승희 (충청남도 축산기술연구소) ;
  • 이승환 (축산과학원 한우시험장) ;
  • 이준헌 (충남대학교 농업생명과학대학 동물자원생명과학과)
  • Received : 2012.07.31
  • Accepted : 2012.09.12
  • Published : 2012.09.30
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Abstract

Thyroglobulin (TG) gene was known to be regulated fat cell growth and differentiation and the endothelial differentiation sphingolipid G-protein-coupled receptor 1 (EDG1) gene involves blood vessel formation and known to be affecting carcass traits in beef cattle. The aim of this study was to identify the single nucleotide polymorphisms (SNPs) in both TG and EDG1 genes and to analyze the association with carcass traits in Korean cattle (Hanwoo). The T354C SNP in TG gene located at the 3' flanking region and c.-312A>G SNP located at 3'-UTR of EDG1 gene were used for genotyping the animals using PCR-RFLP method. Three genotypes were identified in T354C SNP in TG gene and only two AA and AG genotypes were observed for the c.-312A>G SNP in EDG1 gene. The results indicated that T354C SNP in TG gene was not significantly associated with carcass traits. However, the c.-312A>G SNP in EDG1 gene had significant effects on backfat thickness (BF) and yield index (YI). These results may provide valuable information for further candidate gene studies affecting carcass traits in Korean cattle and may use as marker assisted selection for improving the quality of meat in Hanwoo.

Keywords

References

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