Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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The protective effect of Perilla frutescens from ONOO--induced oxidative stress and antiaging effect under cellular system

Cellular system에서의 깻잎의 ONOO-에 의한 산화적 스트레스 개선 및 항노화 효과

  • Kim, Hyun Young (Department of Food Science, Gyeongnam National University of Science and Technology) ;
  • Hwang, Bo Ra (Department of Food Science and Nutrition & Research Institute of Ecology for the Elderly, Pusan National University) ;
  • Wu, Ting Ting (Department of Food Science and Nutrition & Research Institute of Ecology for the Elderly, Pusan National University) ;
  • Cho, Eun Ju (Department of Food Science and Nutrition & Research Institute of Ecology for the Elderly, Pusan National University)
  • 김현영 (경남과학기술대학교 식품과학부) ;
  • 황보라 (부산대학교 식품영양학과 및 노인생활환경연구) ;
  • ;
  • 조은주 (부산대학교 식품영양학과 및 노인생활환경연구)
  • Received : 2012.08.31
  • Accepted : 2012.11.08
  • Published : 2012.12.31
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Abstract

In this study, we investigated the antioxidative and antiaging activity of Perilla frutescens using LLC-$PK_1$ porcine renal epithelial cell and WI-38 human diploid fibroblast cell. The extract from Perilla frutescens showed strong protective effect against nitric oxide (NO) and superoxide ($O_2{^-}$)-induced oxidative stress generated by sodium nitroprusside (SNP) and pyrogallol, respectively. The result showed that P. frutescens increased the cell viability and showed scavenging activity of NO and $O_2{^-}$. In addition, the extract of P. frutescens exerted the protective effect against peroxynitrite ($ONOO^-$) induced by 3-morpholinosydnonimine. It suggests that P. frutescens would have the protective role against $ONOO^-$ itself and its precursors, NO and $O_2{^-}$. Furthermore, the aging model of hydrogen peroxide ($H_2O_2$)-treated WI-38 human diploid fibroblast was employed to investigate the anti-aging effect of P. frutescens. $H_2O_2$-treated WI-38 cells showed the loss of cell viability, however before-treatment with P. frutescens to WI-38 cells under premature senescence could delay the cellular aging process. The present study suggests the antioxidative and antiaging potential against free radical-induced oxidative damage of P. frutescens.

Keywords

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