Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
권호 표지
DOI QR코드

DOI QR Code

Development of new broccoli varieties from elite lines obtained by microspore cultivation method

소포자 배양 유래 계통을 활용한 브로콜리 신품종 조기 육성

  • Kwak, Jung-Ho (National Institute of Horticultural and Herbal Science, RDA) ;
  • Park, Miyoung (National Institute of Horticultural and Herbal Science, RDA) ;
  • Lee, Jun-Gu (National Institute of Horticultural and Herbal Science, RDA) ;
  • Park, Suhyung (National Institute of Horticultural and Herbal Science, RDA) ;
  • Kim, Dae-Young (National Institute of Horticultural and Herbal Science, RDA) ;
  • Jeong, Seung-Ryong (National Institute of Horticultural and Herbal Science, RDA) ;
  • Lim, Yong Pyo (Chungnam National University, Dept. of Horticultural Science) ;
  • Yoon, Moo Kyoung (National Institute of Horticultural and Herbal Science, RDA)
  • 곽정호 (농촌진흥청 국립원예특작과학원) ;
  • 박미영 (농촌진흥청 국립원예특작과학원) ;
  • 이준구 (농촌진흥청 국립원예특작과학원) ;
  • 박수형 (농촌진흥청 국립원예특작과학원) ;
  • 김대영 (농촌진흥청 국립원예특작과학원) ;
  • 정승룡 (농촌진흥청 국립원예특작과학원) ;
  • 임용표 (충남대학교 원예학과) ;
  • 윤무경 (농촌진흥청 국립원예특작과학원)
  • Received : 2012.11.13
  • Accepted : 2012.12.24
  • Published : 2012.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Since the year 2000, the production and consumption of broccoli have rapidly increased in Korea. And, the average production area and amount were about 1,700 ha and 29,000 ton for the past 5 years. Even with the increase of these cultivation and consumption, more than 95% of the broccoli seeds are currently imported from foreign countries such as Japan and Netherlands. Therefore, development of domestic broccoli varieties is needed to relieve Korean farmers' production cost for broccoli. In this situation, National Institute of Horticultural and Herbal Science (NIHHS) of Korea has tried to develop F1 hybrid varieties from elite lines that were obtained by microspore cultivation method from 2008. As the results, about 850 lines of broccoli were obtained and self-pollinated. Then their ploidy levels of the genome were confirmed to select double haploid (D.H.) lines. And the D.H. lines' horticultural traits were evaluated in open field. After the selection of 17 elite D.H. lines, they were cross-pollinated with a male sterile (MS) line to produce F1 hybrid seeds. After 2 to 3 years field trials of these F1 hybrid varieties at the area of Suwon, Gangneung, and Jeju respectively, two hybrid varieties such as 'Wongyo8011' and 'Wongyo8012' are selected for the application of variety protection. With these 4 years of research, we found that the microspore cultivation method is a powerful tool for the conventional breeding program, especially for the development of various inbred lines and even F1 hybrid varieties in short time.

Keywords

References

  1. Biggs AG. 1985. Vegetable Seed Production. In: Principles of Vegetable Crop Production edited by Fordham R. pp. 198-209. Collins, London, UK.
  2. Clement C, Sangwan RS, Sangqan-Norreel B. 2005. Microspore embryo induction and development in higher plants. Biotechnology in Agriculture and Forestry 56:53-72. https://doi.org/10.1007/3-540-26889-8_4
  3. Gamborg OL. Miller RA. Ojima K. 1968. Nutrient requirements of suspension cultures of soybean root cells. Experimental Cell Research 50:151-158. https://doi.org/10.1016/0014-4827(68)90403-5
  4. Jang SU, 2003. The Cultivation of Cauliflower and Bbroccoli. In: The Cultivation of Western Vegetable edited by Kim Y.U. pp. 95-149. Sammi, Suwon, Korea.
  5. Keller WA. Armstrong KC. 1979. Stimulation of embryogenesis and haploid production in Brassica campestris anther cultures by elevated temperature treatments. Theoretical and Applied Genetics 55:65-67.
  6. Kwak JH, Park MY, Kim KT, Yon MK, Park S, Kim DY, Cheong SR. 2012. Analysis of Horticultural Character and Genetic Relatedness of Broccoli Germplasm and Lines. Korean Journal of Horticultural Science & Technology 30 (suppl. II):88.
  7. Lichter R. 1982. Induction of haploid plants from isolated pollen of Brassicanapus. Zeitschrift fur Pflanzenphysiologie 105:427-434. https://doi.org/10.1016/S0044-328X(82)80040-8
  8. MFAFF(Ministry for Food, Agriculture, Forestry and Fisheries). 2012. Assessed in http://www.mifaff.go.kr/main.jsp on 5 November 2012.
  9. Na H. Kwak JH. Chun CH. 2011a. The effects of plant growth regulators, activated charcoal, and AgNO3 on microspore derived embryo formation in broccoli (Brassica oleracea L. var italica). Horticulture, Environment, and Biotechnology 52(5):524-529. https://doi.org/10.1007/s13580-011-0034-7
  10. Na H. Hwang G. Kwak JH. Yoon MK. Chun CH. 2011b. Microspore derived embryo formation and doubled haploid plant production in broccoli (Brassica oleracea L. var italica) according to nutritional and environmental conditions. African Journal of Biotechnology 10(59):12535-12541.
  11. Takahata Y. Keller, WA. 1991. High frequency embryogenesis and plant regeneration in isolated microspore culture of Brassica oleracea L. Plant Science 74:232-242.
  12. Vicente JG. Dias JS. 1996. Production of embryos from microspore cultures of Portuguese tronchuda cabbage landraces. Acta Horticulturae 407:219-226.
  13. Wien HC, Wurr DCE. 1997. Cauliflower, Broccoli, Cabbage and Brussels sprouts. In: The Physiology of Vegetable Crops edited by Wein H.C. pp.511-552. CAB International, Oxon, UK.
  14. Yuan SX. Liu YM. Fang ZY. 2011. Effect of Combined Cold Pretreatment and Heat Shock on Microspore Cultures in Broccoli. Plant Breeding 130(1):80-85 https://doi.org/10.1111/j.1439-0523.2009.01754.x