Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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Isolation and identification of antifungal compounds from Reynoutria elliptica

호장근(Reynoutria elliptica)으로부터 항균활성 물질의 분리 및 구조결정

  • Hwang, Joo-Tae (Department of Bio Environmental Chemistry, Chungnam National University) ;
  • Park, Young-Sik (Department of Bio Environmental Chemistry, Chungnam National University) ;
  • Kim, Young-Shin (Department of Bio Environmental Chemistry, Chungnam National University) ;
  • Kim, Jin-Cheol (Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology) ;
  • Lim, Chi-Hwan (Department of Bio Environmental Chemistry, Chungnam National University)
  • 황주태 (충남대학교 생물환경화학과) ;
  • 박영식 (충남대학교 생물환경화학과) ;
  • 김영신 (충남대학교 생물환경화학과) ;
  • 김진철 (한국화학연구원 바이오 정밀화학연구센터) ;
  • 임치환 (충남대학교 생물환경화학과)
  • Received : 2012.11.12
  • Accepted : 2012.12.12
  • Published : 2012.12.31
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Abstract

In the continued research on natural fungicides for the control of plant diseases by using plant-derived products, we found that Reynoutria elliptica. had a strong fungicidal activity against several plant pathogens. R. elliptica (3.2 kg) were extracted with 80% aq. MeOH and the concentrated extracted was partitioned with n-hexane, EtOAc, n-BuOH and $H_2O$ successively. The four layers were tested their disease control efficacies against 4 plant disease such as rise blast (RCB), tomato grey mold (TGM), tomato late blight (TLB), and barly powdery mildew (BPM). The n-hexane fraction was highly active showing over 95% control against TLB and BPM. and the EtOAc fraction was highly active showing over 95% control against RCB, TLB, and BPM. By using silica gel chromatography, MPLC, and HPLC, three compounds that were expected to have antifungal activity were isolated. Their chemical structures were elucidated as physcion, emodin, and emodie-8-O-glucoside by EI-MS and NMR spectroscopic analyses.

Keywords

References

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