Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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The selection of basic platform for improving the sensitivity of neutravidin rapid detection kit

뉴트라비딘 검출용 간이 진단키트의 성능향상을 위한 기본 플랫폼 선정

  • Choi, Sunmi (Biosensor Lab., Dept. of Agricultural Engineering, National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Giyoung (Biosensor Lab., Dept. of Agricultural Engineering, National Academy of Agricultural Science, Rural Development Administration) ;
  • Om, Aeson (Food Safety & Toxicology Lab., Dept. of Food & Nutrition, Hanyang University) ;
  • Moon, Jihea (Biosensor Lab., Dept. of Agricultural Engineering, National Academy of Agricultural Science, Rural Development Administration) ;
  • Park, Saetbyeol (Biosensor Lab., Dept. of Agricultural Engineering, National Academy of Agricultural Science, Rural Development Administration) ;
  • Lee, Sangdae (Biosensor Lab., Dept. of Agricultural Engineering, National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Hyuk Joo (Biosensor Lab., Dept. of Agricultural Engineering, National Academy of Agricultural Science, Rural Development Administration)
  • 최선미 (농촌진흥청 국립농업과학원 농업공학부 바이오센서연구실) ;
  • 김기영 (농촌진흥청 국립농업과학원 농업공학부 바이오센서연구실) ;
  • 엄애선 (한양대학교 식품영양학과 식품 안전 및 독성연구실) ;
  • 문지혜 (농촌진흥청 국립농업과학원 농업공학부 바이오센서연구실) ;
  • 박샛별 (농촌진흥청 국립농업과학원 농업공학부 바이오센서연구실) ;
  • 이상대 (농촌진흥청 국립농업과학원 농업공학부 바이오센서연구실) ;
  • 김혁주 (농촌진흥청 국립농업과학원 농업공학부 바이오센서연구실)
  • Received : 2012.09.02
  • Accepted : 2012.11.14
  • Published : 2012.12.31
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Abstract

This study was performed to optimize the basic platform of a lateral flow immunoassay. Improvement of the limit of detection (LOD) was evaluated according to the width of a nitrocellulose membrane with varying concentrations of analyte. The analyte, neutravidin was detected based on the avidin-biotin interaction. The antibody-Au nanoparticle conjugation was mostly stabled in a PBS buffer of pH 7.3. The optimal widths of a nitrocellulose membrane were 4 and 6 mm considering the sample flow rate and signal strength of the test line on the membrane. The LOD of neutravidin was 0.001 mg/ml in the optimum conditions.

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References

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