Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Isolation and Identification of Lactic Acid Bacteria from Spent Mushroom Substrate for Silage Making and Determination of Optimal Medium Conditions for Growth

  • Kim, Young-Il (Animal Science, School of Life Resource and Environmental Sciences, Konkuk University) ;
  • Kwak, Wan-Sup (Animal Science, School of Life Resource and Environmental Sciences, Konkuk University)
  • Received : 2012.06.13
  • Accepted : 2012.11.22
  • Published : 2012.12.31
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Abstract

This study was conducted to isolate and identify the lactic acid bacteria (LAB) from spent mushroom substrates (SMS) for the effective anaerobic fermentation to utilize SMS as an animal feed and to determine the optimal medium conditions for their growth. At first, a total of 23 strains were isolated from the ensiled SMS based on the LAB counts and pH tested. Then, a total of 16 strains which rapidly produce lactate and decreased the pH, were selected for a screening test. The optical density (OD), pH, and yellow clear zone were tested for the selected 16 strains. Among the strains, KU5 strain had wider yellow clear zone and lower pH and KU13 strain had higher OD at 24 hr of incubation and wider yellow clear zone compared to other strains and control strain (Lactobacillus plantarum KCCM 12116). Accordingly, KU5 and KU13 strains were finally selected. The KU5 and KU13 were identified as Lactobacillus plantarum by the 16S rRNA sequencing. The KU5 strain was named as Lactobacillus plantarum KU5, and the KU13 strain was named as Lactobacillus plantarum KU13. Lactobacillus plantarum KU5 and Lactobacillus plantarum KU13 were registered at the National Center for Biotechnology Information (NCBI). Access number of Lactobacillus plantarum KU5 was HQ542227 and that of Lactobacillus plantarum KU13 was HQ542228. The optimal medium conditions for growth of KU5 and KU13 were soybean meal 2% and formulated feed 2%, respectively.

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References

  1. Adesogan, A. T., Salawu, M. B., Ross, A. B., Davies, D. R. and Brooks, A. E. 2003. Effect of Lactobacillus buchneri, L. fermentum or Leuconostoc mesenteroides inoculants or a chemical additive on the fermentation, aerobic stability and nutritive value of crimped wheat grains. J. Dairy Sci. 86: 1789-1796. https://doi.org/10.3168/jds.S0022-0302(03)73764-3
  2. Bolsen, K. K., Lin, C., Brenta, B. E., Feyerherm, A. M., Urbanc, J. E. and Aimutis, W. R. 1992. Effect of silage additives on the microbial succession and fermentation process of alfalfa and corn silages. J. Dairy Sci. 75:3066-3083. https://doi.org/10.3168/jds.S0022-0302(92)78070-9
  3. Claus, D. and Berkeley, R. C. W. 1986. Bergey's Manual of Systematic Bacteriology Vol. II, Williams and Wilkins, Baltimore, USA. p:1104.
  4. Driehuis, F., Elferink, S. J. W. H. O. and Van Wikselaar, P. G. 2001. Fermentation characteristics and aerobic stability of grass silage inoculated with Lactobacillus buchneri, with or without homofermentative lactic acid bacteria. Grass Forage Sci. 56:330-343. https://doi.org/10.1046/j.1365-2494.2001.00282.x
  5. Jung, W. H., Yang, S. Y., Song, M. D., Ha, J. K. and Kim, C. W. 2003. Isolation of bacillus sp. producing xylanase and cellulose and optimization of medium condition for its production. Kor. J. Microbiol. Biotechnol. 21(4):383-388.
  6. Kakkar, V. K. and Dhanda, S. 1998. Comparative evaluation of wheat and paddy straws for mushroom production and feeding residual straws to ruminants. Bioresource Technology 66:175-177. https://doi.org/10.1016/S0960-8524(97)00098-9
  7. Kim, Y. I., Jung, S. H., Seok, J. S., Yang, S. Y., Huh, J. W. and Kwak, W. S. 2007a. Isolation and identification of highly cellulolytic bacteria from spent mushroom substrate and determination of optimal medium conditions for the growth. Kor. J. Microbiol. Biotechnol. 35(3):255-260.
  8. Kim, Y. I., Jung, S. H., Yang, S. Y., Huh, J. W. and Kwak, W. S. 2007b. Effects of cellulolytic microbes inoculation during deep stacking of spent mushroom substrates on cellulolytic enzyme activity and nutrients utilization by sheep. J. Anim. Sci. & Technol. (Kor.). 49(5):667-676. https://doi.org/10.5187/JAST.2007.49.5.667
  9. Kleinschmit, D. H. and Kung, L. Jr. 2006. The effects of Lactobacillus buchneri 40788 and Pediococcus pentosaceus R1094 on the fermentation of corn silage. J. Dairy Sci. 89: 3999-4004. https://doi.org/10.3168/jds.S0022-0302(06)72443-2
  10. Kwak, W. S., Jung, S. H. and Kim, Y. I. 2008. Broiler litter supplementation improves storage and feed-nutritional value of sawdust-based spent mushroom substrate. Bioresource Technology 99:2947-2955. https://doi.org/10.1016/j.biortech.2007.06.021
  11. Lee, S. M., Kim, Y. I., and Kwak, W. S. 2010. Effect of by-product mixing silage feeding on the eating and ruminating behavior of Hanwoo steer. J. Kor. Grassl. Forage Sci. 30(2):159-168. https://doi.org/10.5333/KGFS.2010.30.2.159
  12. Suzuki, Y, Okano, K. and Kato, S. 1995. Characteristics of white-rotted woody materials obtained from Shiitake mushroom (Lentinus edodes) and Nameko mushroom (Pholiota nameko) cultivation with in vitro rumen fermentation. Anim. Feed Sci. and Tech. 54:227-236. https://doi.org/10.1016/0377-8401(95)00769-J
  13. Thompson, J. D., Higgins, D. G. and Gibson, T. J. 1994. Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22: 4673-3680. https://doi.org/10.1093/nar/22.22.4673
  14. Weinberg, Z. G. and Muck, R. E. 1996. New trends and opportunities in the development and use of inoculants for silage. FEMS Microbiol. Rev. 19:53-68.
  15. Yang, S. Y., Ji, K. S., Baik, Y. H., Kwak, W. S. and McCaskey, T. A. 2006. Lactic acid fermentation of food waste for swine feed. Bioresource Technology 97:1858-1864. https://doi.org/10.1016/j.biortech.2005.08.020
  16. Yang, S. Y., Song, M. D., Kim, O. H. and Kim, C. W. 2001. Isolation of bacillus sp. producing multi - enzyme and optimization of medium conditions for its production using feedstuffs for probiotics. Kor. J. Microbiol. Biotechnol. 29:110-114.
  17. Zhang, C. K., Gong, F. and Li, D. S. 1995. A note on the utilization of spent mushroom composts in animal feeds. Bioresource Technology 52:89-91. https://doi.org/10.1016/0960-8524(94)00137-P