Korean Journal of Acupuncture

Society for Meridian and Acupoint (경락경혈학회)
권호 표지

Effects of Low Level Laser Treatment at LR2 and LR8 acupoint on the liver damage induced in D-GalN in rats

간경(肝經)의 형화혈(滎火穴)과 합수혈(合水穴)에 시술한 레이저침이 D-GalN 간손상 유발 흰쥐에 미치는 영향

  • Kim, Wang-In (Dept. of Meridian & Acupoint, College of Oriental Medicine, Dongshin University) ;
  • Youn, Dae-Hwan (Dept. of Meridian & Acupoint, College of Oriental Medicine, Dongshin University) ;
  • Choi, Chan-Hun (Dept. of Physiology, College of Oriental Medicine, Dongshin University) ;
  • Na, Chang-Su (Dept. of Meridian & Acupoint, College of Oriental Medicine, Dongshin University)
  • 김왕인 (동신대학교 한의학과 경혈학교실) ;
  • 윤대환 (동신대학교 한의학과 경혈학교실) ;
  • 최찬헌 (동신대학교 한의학과 생리학교실) ;
  • 나창수 (동신대학교 한의학과 경혈학교실)
  • Received : 2012.03.02
  • Accepted : 2012.03.16
  • Published : 2012.03.27
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Abstract

Objectives : This study was performed to investigate the effect of invasive laser acupuncture treatment at Liver Brook (LR2) acupoint and Liver Sea (LR8) acupoint on liver damage induced by D-galactosamine (D-GalN) in rats Methods : Liver damage was induced by D-GalN. The experimental rats were divided into two groups (control group, Low Level Laser Treatment (LLLT) group). Control groups were classified into small groups. Intact group had no liver damage and no treatment. D-GalN group was induced liver damage induced by D-GalN and not treated. LLLT group were induced liver damage induced by D-GalN and then treated at the LR2 or LR8 acupoint with 532, 658, 904 nm invasive laser acupuncture. The treatment was carried out three days at a time for 15days at both acupoints. To examine mechanism of the effect of invasive laser acupuncture, we measured the contents of ASP, ALT, ALP, TBIL in serum, CBC in blood and SOD in liver tissue. Results : The change of body weight increased in all groups. That change was AST and ALP, the AST activity decreased significantly compared with the control groups and decreased by 532 nm and 904 nm both LLLT groups. But ALP increased at LR8 acupoint by 658 nm. TBIL level significantly decreased in all LLLT groups. The SOD of LLLT groups increased in the liver tissue of rats compared to the control groups. SOD activity indicated that LLLT can help cellular defense mechanism by preventing scavenging hydrogen peroxide. In the change of WBC, it was increased in D-GalN Control group compared to intact group and LLLT groups. Conclusions : These results suggested that invasive laser acupuncture treatment at LR2 or LR8 acupoint reduced activation of hepatic enzyme and damage of liver tissue. Thus, the effect of invasive laser acupuncture was nearly identical to the way of the traditional acupuncture for the treatment of hepatocytotoxicity.

Keywords

References

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