The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Identification of the Capsid Protein-binding Region of the SL1(+) RNA Located at the 5' Region of the Potato virus X Genome

  • Cho, Sang-Yun (Department of Agricultural Biotechnology and Plant Genomics and Breeding Institute, Seoul National University) ;
  • Kim, Kook-Hyung (Department of Agricultural Biotechnology and Plant Genomics and Breeding Institute, Seoul National University)
  • Received : 2011.11.23
  • Accepted : 2011.11.27
  • Published : 2012.03.01
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Abstract

$Potato$ $virus$ $X$ (PVX) contains $cis$-acting elements including stem-loop 1 (SL1) RNA at the 5' region; SL1 is conserved among all potexviruses. The SL1 at the positive-sense RNA, SL1(+), is required for PVX RNA replication, cell-to-cell movement, and translation. Previous research demonstrated that SL1(+) RNA also serves as the origin of assembly for encapsidation of PVX RNA. To identify the essential sequences and/or regions for capsid protein (CP) subunit recognition within SL1(+) RNA, we used electrophoretic mobility shift assays (EMSA), UV cross-linking, and yeast three-hybrid analyses. The EMSA and UV cross-linking analyses with PVX CP subunits and RNA transcripts corresponding to the SL1(+) RNA showed that the SL1(+) RNA formed complexes with CP subunits. We also conducted EMSA and yeast three-hybrid analyses with RNAs containing various mutations of SL1(+) RNA elements. These analyses indicated that SL1(+) RNA is required for the interaction with PVX CP and that the RNA sequences located at the loop C and tetra loop of the SL1(+) are crucial for CP binding. These results indicate that, in addition to being important for RNA accumulation, the SL1(+) RNA from the 5' region of the PVX genome is also required for specific binding of PVX CP.

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References

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