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FGF-2 inhibits TNF-α mediated apoptosis through up-regulation of Bcl2-A1 and Bcl-xL in ATDC5 cells

  • Kim, Hey-Ryun (Division of Biochemistry, College of Medicine, Chungbuk National University) ;
  • Heo, Youn-Moo (Division of Orthopaedic Surgery, College of Medicine, Chungbuk National University) ;
  • Jeong, Kyoung-Il (Division of Orthopaedic Surgery, College of Medicine, Chungbuk National University) ;
  • Kim, Yong-Min (Division of Orthopaedic Surgery, College of Medicine, Chungbuk National University) ;
  • Jang, Hae-Lan (Division of Medical Infomation and Management, College of Medicine, Chungbuk National University) ;
  • Lee, Kwang-Yeol (College of Pharmacy, Chonnam National University) ;
  • Yeo, Chang-Yeol (Department of Life Science and Division of Life and Pharmaceutical Sciences, Ewha Womans University) ;
  • Kim, Sung-Hoon (Genomic Informatics Center, Hankyong National University) ;
  • Lee, Hak-Kyo (Genomic Informatics Center, Hankyong National University) ;
  • Kim, Seung-Ryul (Division of Biochemistry, College of Medicine, Chungbuk National University) ;
  • Kim, Eung-Gook (Division of Biochemistry, College of Medicine, Chungbuk National University) ;
  • Choi, Joong-Kook (Division of Biochemistry, College of Medicine, Chungbuk National University)
  • Received : 2012.01.03
  • Accepted : 2012.01.16
  • Published : 2012.05.31

Abstract

FGF-2 is involved in cell survival, proliferation, apoptosis, and angiogenesis in a wide variety of cells. FRGRs, PI3K and MAP kinases are well known mediators of FGF signaling. Despite its known roles during many developmental processes, including osteogenesis, there are few known targets of FGF-2. In the present study, we identified Bcl2-A1 and Bcl-xL as two prominent targets involved in promoting cell survival. Pretreatment of ATDC5 cells with FGF-2 increased cell survival, while siRNAs specific for Bcl2-A1 and Bcl-xL compromised the anti-apoptotic effect of FGF-2, sensitized the cells to apoptosis triggered by TNF-${\alpha}$. Chemical inhibition of FGFR, NFkB, and PI3K activity by PD173074, pyrrolidine dithiocarbamate, and LY294002 respectively abrogated the FGF-2-mediated induction of Bcl2-A1 and Bcl-xL expression. Taken together, our data demonstrate that a subset of Bcl2 family proteins are the targets of FGF-2 signaling that promotes the survival of ATDC5 cells.

Keywords

References

  1. Glaser, R. L., Broman, K. W., Schulman, R. L., Eskenazi, B., Wyrobek, A. J. and Jabs, E. W. (2003) The paternal-age effect in Apert syndrome is due, in part, to the increased frequency of mutation in sperm. Am. J. Hum. Genet. 73, 939-947. https://doi.org/10.1086/378419
  2. Goriely, A., McVean, G. A., Rojmyr, M., Ingemarsson, B. and Wilkie, A. O. (2003) Evidence for selective advantage of pathogenic FGFR2 mutations in the male germ line. Science 301, 643. https://doi.org/10.1126/science.1085710
  3. Feldman, B., Poueymirou, W., Papaioannou, V. E., DeChiara, T. M. and Goldfarb, M. (1995) Requirement of FGF-4 for postimplantation mouse development. Science 267, 246- 249. https://doi.org/10.1126/science.7809630
  4. Colvin, J. S., White, A. C., Pratt, S. J. and Ornitz, D. M. (2001) Lung hypoplasia and neonatal death in Fgf9-null mice identify this gene as an essential regulator of lung mesenchyme. Development 128, 2095.
  5. McIntosh, I., Bellus, G. A. and Jab, E. W. (2000) The pleiotropic effects of fibroblast growth factor receptors in mammalian development. Cell Struct. Funct. 25, 85-96. https://doi.org/10.1247/csf.25.85
  6. Pardo, O. E., Lesay, A., Arcaro, A., Lopes, R., Ng, B. L., Warne, P. H., McNeish, I. A., Tetley, T. D., Lemoine, N. R., Mehmet, H., Seckl, M. J. and Downward, J. (2003) Fibroblast growth factor 2-mediated translational control of IAPs blocks mitochondrial release of Smac/DIABLO and apoptosis in small cell lung cancer cells. Mol. Cell Biol. 23, 7600-7610. https://doi.org/10.1128/MCB.23.21.7600-7610.2003
  7. Chia, S. L., Sawaji, Y., Burleigh, A., McLean, C., Inglis, J., Saklatvala, J. and Vincent, T. (2009) Fibroblast growth factor 2 is an intrinsic chondroprotective agent that suppresses ADAMTS-5 and delays cartilage degradation in murine osteoarthritis. Arthritis Rheum. 60, 2019-2027. https://doi.org/10.1002/art.24654
  8. Makino, T., Jinnin, M., Muchemwa, F. C., Fukushima, S., Kogushi-Nishi, H., Moriya, C., Igata, T., Fujisawa, A., Johno, T. and Ihn, H. (2010) Basic fibroblast growth factor stimulates the proliferation of human dermal fibroblasts via the ERK1/2 and JNK pathways. Br. J. Dermatol. 62, 717- 723.
  9. Sahni, M., Ambrosetti, D. C., Mansukhani, A., Gertner, R., Levy, D. and Basilico, C. (1999) FGF signaling inhibits chondrocyte proliferation and regulates bone development through the STAT-1 pathway. Genes Dev. 13, 1361-1366. https://doi.org/10.1101/gad.13.11.1361
  10. Krejci, P., Salazar, L., Goodridge, H. S., Kashiwada, T. A., Schibler, M. J., Jelinkova, P., Thompson, L. M. and Wilcox, W. R. (2008) STAT1 and STAT3 do not participate in FGFmediated growth arrest in chondrocytes. J. Cell Sci. 121, 272-281. https://doi.org/10.1242/jcs.017160
  11. Youle, R. J. and Strasser, A. (2008) The BCL-2 protein family: opposing activities that mediate cell death. Nat. Rev. Mol. Cell Biol. 9, 47-59. https://doi.org/10.1038/nrm2308
  12. Lin, E. Y., Orlofsky, A., Berger, M. S. and Prystowsky, M. B. (1993) Characterization of A1, a novel hemopoietic-specific early-response gene with sequence similarity to bcl-2. J. Immunol. 151, 1979-1988.
  13. Karsan, A., Yee, E. and Harlan, J. M. (1996) Endothelial cell death induced by tumor necrosis factor-alpha is inhibited by the Bcl-2 family member, A1. J. Biol. Chem. 271, 27201-27204. https://doi.org/10.1074/jbc.271.44.27201
  14. Cross, A., Moots, R. J. and Edwards, S. (2008) The dual effects of TNFalpha on neutrophil apoptosis are mediated via differential effects on expression of Mcl-1 and Bfl-1. Blood 111, 878-884. https://doi.org/10.1182/blood-2007-05-087833
  15. Tare, B. S., Howard, D., Pound, J. C., Roach, H. I. and Oreffo, R. O. C. (2005) ATDC5: an ideal cell line for development of tissue engineering strategies aimed at cartilage generation. Eur. Cell. Mater. 10(Suppl 2), 22.
  16. Im, Y. S., Shin, H. K., Kim, H. R., Jeong, S. H., Kim, S. R., Kim, Y. M., Lee, D. H., Jeon, S. H., Lee, H. W. and Choi, J. K. (2009) Enhanced cytotoxicity of 5-FU by bFGF through up-regulation of uridine phosphorylase 1. Mol. Cells 28, 119-124. https://doi.org/10.1007/s10059-009-0116-x
  17. Reers, M., Smith, T. W. and Chen, L. B. (1991) J-aggregate formation of a carbocyanine as a quantitative fluorescent indicator of membrane potential. Biochemistry 7, 4480- 4486.
  18. Shirane, M. and Nakayama, K. I. (2003) Inherent calcineurin inhibitor FKBP38 targets Bcl-2 to mitochondria and inhibits apoptosis. Nat. Cell Biol. 5, 28-37. https://doi.org/10.1038/ncb894
  19. Choi, B. H., Feng, L. and Yoon, H. S. (2010) FKBP38 protects Bcl-2 from caspase-dependent degradation. J. Biol. Chem. 285, 9770-9779. https://doi.org/10.1074/jbc.M109.032466
  20. Fiebig, A. A., Zhu, W., Hollerbach, C., Leber, B. and Andrews, D. W. (2006) Bcl-XL is qualitatively different from and ten times more effective than Bcl-2 when expressed in a breast cancer cell line. BMC Cancer 6, 213. https://doi.org/10.1186/1471-2407-6-213
  21. Vogler, M., Dickens, D., Dyer, M. J., Owen, A., Pirmohamed M. and Cohen G. M. (2011) The B-cell lymphoma 2 (BCL2)-inhibitors, ABT-737 and ABT-263, are substrates for P-glycoprotein. Biochem. Biophys. Res. Commun. 408, 344-349. https://doi.org/10.1016/j.bbrc.2011.04.043
  22. Lee, H. H., Dempsey, P. W., Parks, T. P., Zhu, X., Baltimore, D. and Cheng, G. (1999) Specificities of CD40 signaling: involvement of TRAF2 in CD40-induced NFkappaB activation and intercellular adhesion molecule-1 up-regulation. Proc. Natl. Acad. Sci. U.S.A 96, 1421-1426. https://doi.org/10.1073/pnas.96.4.1421
  23. Duriez, P. J., Wong, F., Dorovini-Zis, K., Shahidi, R. and Karsan, A. (2000) A1 functions at the mitochondria to delay endothelial apoptosis in response to tumor necrosis factor. J. Biol. Chem. 275, 18099-18107. https://doi.org/10.1074/jbc.M908925199
  24. Wang, C. Y., Guttridge, D. C., Mayo, M. W. and Baldwin, A. S. Jr. (1999) NF-kappaB induces expression of the Bcl-2 homologue A1/Bfl-1 to preferentially suppress chemotherapy- induced apoptosis. Mol. Cell Biol. 19, 5923-5929. https://doi.org/10.1128/MCB.19.9.5923
  25. Rastegar, F., Gao, J. L., Shenag, D., Luo, Q., Shi, Q., Kim, S. H., Jiang, W., Wagner, E. R., Huang, E., Gao, Y., Shen, J., Yang, K., He, B. C., Chen, L., Zuo, G. W., Luo, G. W., Luo, J., Luo, X., Bi, Y., Liu, X., Li, M., Hu, N., Wang, L., Luther, G., Luu, H. H., Haydon, R. C. and He, T. C. (2010) Lysophosphatidic acid acyltransferase $\beta$ (LPAAT $\beta$) promotes the tumor growth of human osteosarcoma. PLoS One 5, e14182. https://doi.org/10.1371/journal.pone.0014182

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