Journal of Nutrition and Health

  • 제45권2호
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  • Pages.121-126
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  • 2012
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  • 2288-3886(pISSN)
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  • 2288-3959(eISSN)
한국영양학회 (The Korean Nutrition Society)
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어유의 n-3 지방산이 흰쥐의 골밀도와 골격대사지표에 미치는 영향

Beneficial effect of fish oil on bone mineral density and biomarkers of bone metabolism in rats

  • 윤군애 (동의대학교 식품영양학과)
  • Yoon, Gun-Ae (Department of Food and Nutrition, Dong-Eui University)
  • 투고 : 2011.12.29
  • 심사 : 2012.02.25
  • 발행 : 2012.04.30
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초록

본 연구는 n-3 장쇄지방산이 풍부한 어유가 골격대사에 미치는 영향을 알아보고자 이유한 Sprague-Dawley 종 흰쥐 (수컷)에게 4주 동안 FO군, FICO군, SO군의 실험식이를 공급한 후, 혈액과 뇨에서 생화학적 골격대사지표와 대퇴골의 무기질함량 및 골밀도를 측정하였다. 부갑상선호르몬, 칼시토닌, 오스테오 칼신은 세 그룹 사이에 차이를 보이지 않았으며, 뼈흡수지표인 deoxypyridinoline도 그룹 간에 차이가 없는 것으로 나타났다. 반면에 골격형성지표인 ALP의 활성은 FO군에서 SO군에 비해 유의하게 높았고, 대퇴골의 골밀도 또한 SO군에 비해 FO군에서 유의하게 높은 값을 보였다. 그러나 FICO군과 SO군 간에는 차이가 없는 것으로 나타났다. 이러한 결과로 볼 때, 본 연구에서 DHA, EPA 급원의 어유 함량이 높은 식이는 ALP활성을 높이고 골밀도를 증대시킴으로써 성장기 흰쥐의 골격형성에 보호적인 효과가 있음을 알 수 있다. 그러나 식이 n-3 장쇄지방산이 골격대사에 미치는 효과를 규정하기 위해서는 적정식이수준이나 적정 n6/n3 비율과 관련된 많은 연구가 요구되며, n-3계 내의 각 지방산이 골격에 주는 작용양상에도 차이가 있을 수 있으므로 이에 대한 다양한 연구의 병행이 필요할 것으로 본다.

This study evaluated the effect of fish oil rich in n-3 fatty acids on bone characteristics in Sprague-Dawley rats. Weanling male rats were randomized to receive either a diet containing high fish oil (FO), fish oil blended with corn oil (FICO), or soy oil rich in n-6 fatty acids (SO) for 4 weeks. All diets provided 70 g/kg fat based on the AIN-93G diet. Growth and biomarkers of bone metabolism were analyzed, and femur bone characteristics were measured by dual-energy X-ray absorptiometry. After the dietary treatment, no significant differences among the diet groups were observed for serum concentrations of Ca, parathyroid hormone, calcitonin, or osteocalcin. Alkaline phosphatase activity was significantly greater in FO-fed rats compared to that in the FICO and SO groups, whereas no difference in deoxypyridinoline values was observed, supporting the positive effect of a FO diet on bone formation. These results were accompanied by a significant increase in femur bone mineral density (BMD) in FO-fed rats. These findings suggest that providing fish oil rich in n-3 fatty acids correlates with higher alkaline phosphatase activity and BMD values, favoring bone formation in growing rats.

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참고문헌

  1. Statistics Korea. Population projections for Korea: 2010-2060; 2011
  2. Sun D, Krishnan A, Zaman K, Lawrence R, Bhattacharya A, Fernandes G. Dietary n-3 fatty acids decrease osteoclastogenesis and loss of bone mass in ovariectomized mice. J Bone Miner Res 2003; 18(7): 1206-1216 https://doi.org/10.1359/jbmr.2003.18.7.1206
  3. Weaver CM. Adolescence: the period of dramatic bone growth. Endocrine 2002; 17(1): 43-48 https://doi.org/10.1385/ENDO:17:1:43
  4. Watkins BA, Li Y, Lippman HE, Seifert MF. Omega-3 polyunsaturated fatty acids and skeletal health. Exp Biol Med (Maywood) 2001; 226(6): 485-497 https://doi.org/10.1177/153537020122600601
  5. Simopoulos AP. The importance of the ratio of omega-6/omega-3 essential fatty acids. Biomed Pharmacother 2002; 56(8): 365-379 https://doi.org/10.1016/S0753-3322(02)00253-6
  6. Weiss LA, Barrett-Connor E, von Mühlen D. Ratio of n-6 to n-3 fatty acids and bone mineral density in older adults: the Rancho Bernardo Study. Am J Clin Nutr 2005; 81(4): 934-938 https://doi.org/10.1093/ajcn/81.4.934
  7. Raisz LG. Pathogenesis of osteoporosis: concepts, conflicts, and prospects. J Clin Invest 2005; 115(12): 3318-3325 https://doi.org/10.1172/JCI27071
  8. Watkins BA, Li Y, Allen KG, Hoffmann WE, Seifert MF. Dietary ratio of (n-6)/(n-3) polyunsaturated fatty acids alters the fatty acid composition of bone compartments and biomarkers of bone formation in rats. J Nutr 2000; 130(9): 2274-2284 https://doi.org/10.1093/jn/130.9.2274
  9. Green KH, Wong SC, Weiler HA. The effect of dietary n-3 longchain polyunsaturated fatty acids on femur mineral density and biomarkers of bone metabolism in healthy, diabetic and dietaryrestricted growing rats. Prostaglandins Leukot Essent Fatty Acids 2004; 71(2): 121-130 https://doi.org/10.1016/j.plefa.2004.03.001
  10. Fernandes G, Lawrence R, Sun D. Protective role of n-3 lipids and soy protein in osteoporosis. Prostaglandins Leukot Essent Fatty Acids 2003; 68(6): 361-372 https://doi.org/10.1016/S0952-3278(03)00060-7
  11. Heaney RP, Abrams S, Dawson-Hughes B, Looker A, Marcus R, Matkovic V, Weaver C. Peak bone mass. Osteoporos Int 2000; 11(12): 985-1009
  12. Poulsen RC, Moughan PJ, Kruger MC. Long-chain polyunsaturated fatty acids and the regulation of bone metabolism. Exp Biol Med (Maywood) 2007; 232(10): 1275-1288 https://doi.org/10.3181/0704-MR-100
  13. Austin LA, Heath H 3rd. Calcitonin: physiology and pathophysiology. N Engl J Med 1981; 304(5): 269-278 https://doi.org/10.1056/NEJM198101293040505
  14. Weaver CM, Peacock M, Martin BR, McCabe GP, Zhao J, Smith DL, Wastney ME. Quantification of biochemical markers of bone turnover by kinetic measures of bone formation and resorption in young healthy females. J Bone Miner Res 1997; 12(10): 1714- 1720 https://doi.org/10.1359/jbmr.1997.12.10.1714
  15. Price PA, Parthemore JG, Deftos LJ. New biochemical marker for bone metabolism. Measurement by radioimmunoassay of bone GLA protein in the plasma of normal subjects and patients with bone disease. J Clin Invest 1980; 66(5): 878-883 https://doi.org/10.1172/JCI109954
  16. Ohishi T, Takahashi M, Kawana K, Aoshima H, Hoshino H, Horiuchi K, Kushida K, Inoue T. Age-related changes of urinary pyridinoline and deoxypyridinoline in Japanese subjects. Clin Invest Med 1993; 16(5): 319-325
  17. Kim YM, Yoon GA, Hwang HJ, Chi GY, Son BY, Bae SY, Kim IY, Chung JY. Effect of bluefin tuna bone on calcium metabolism of the rat. J Korean Soc Food Sci Nutr 2004; 33(1): 101-106 https://doi.org/10.3746/jkfn.2004.33.1.101
  18. Bhattacharya A, Rahman M, Sun D, Fernandes G. Effect of fish oil on bone mineral density in aging C57BL/6 female mice. J Nutr Biochem 2007; 18(6): 372-379 https://doi.org/10.1016/j.jnutbio.2006.07.002
  19. Watkins BA, Li Y, Lippman HE, Feng S. Modulatory effect of omega-3 polyunsaturated fatty acids on osteoblast function and bone metabolism. Prostaglandins Leukot Essent Fatty Acids 2003; 68(6): 387-398 https://doi.org/10.1016/S0952-3278(03)00063-2
  20. Modrowski D, del Pozo E, Miravet L. Dynamics of circulating osteocalcin in rats during growth and under experimental conditions. Horm Metab Res 1992; 24(10): 474-477 https://doi.org/10.1055/s-2007-1003366
  21. Lee AJ, Hodges S, Eastell R. Measurement of osteocalcin. Ann Clin Biochem 2000; 37(Pt 4): 432-446 https://doi.org/10.1177/000456320003700402
  22. Weaver CM. Use of calcium tracers and biomarkers to determine calcium kinetics and bone turnover. Bone 1998; 22(5 Suppl): 103S- 104S https://doi.org/10.1016/S8756-3282(98)00015-5
  23. Poulsen RC, Kruger MC. Detrimental effect of eicosapentaenoic acid supplementation on bone following ovariectomy in rats. Prostaglandins Leukot Essent Fatty Acids 2006; 75(6): 419-427 https://doi.org/10.1016/j.plefa.2006.08.003
  24. Kruger MC, Schollum LM. Is docosahexaenoic acid more effective than eicosapentaenoic acid for increasing calcium bioavailability? Prostaglandins Leukot Essent Fatty Acids 2005; 73(5): 327-334 https://doi.org/10.1016/j.plefa.2005.08.001
  25. Kruger MC, Horrobin DF. Calcium metabolism, osteoporosis and essential fatty acids: a review. Prog Lipid Res 1997; 36(2-3): 131- 151 https://doi.org/10.1016/S0163-7827(97)00007-6
  26. Haag M, Magada ON, Claassen N, Böhmer LH, Kruger MC. Omega-3 fatty acids modulate ATPases involved in duodenal Ca absorption. Prostaglandins Leukot Essent Fatty Acids 2003; 68(6): 423-429 https://doi.org/10.1016/S0952-3278(03)00067-X
  27. Kruger MC, Coetzer H, de Winter R, Gericke G, van Papendorp DH. Calcium, gamma-linolenic acid and eicosapentaenoic acid supplementation in senile osteoporosis. Aging (Milano) 1998; 10 (5): 385-394
  28. Baird HT, Eggett DL, Fullmer S. Varying ratios of omega-6: omega-3 fatty acids on the pre-and postmortem bone mineral density, bone ash, and bone breaking strength of laying chickens. Poult Sci 2008; 87(2): 323-328 https://doi.org/10.3382/ps.2007-00186
  29. Sirois I, Cheung AM, Ward WE. Biomechanical bone strength and bone mass in young male and female rats fed a fish oil diet. Prostaglandins Leukot Essent Fatty Acids 2003; 68(6): 415-421 https://doi.org/10.1016/S0952-3278(03)00066-8
  30. Claassen N, Coetzer H, Steinmann CM, Kruger MC. The effect of different n-6/n-3 essential fatty acid ratios on calcium balance and bone in rats. Prostaglandins Leukot Essent Fatty Acids 1995; 53(1): 13-19 https://doi.org/10.1016/0952-3278(95)90078-0

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