Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
권호 표지

Relationship between Semen Quality Parameters and Field Fertility of Bulls

  • Rabidas, Sushanto Kumar (Department of Surgery and Obstetrics, Faculty of Veterinary Science, Bangladesh Agricultural University) ;
  • Talukder, Anup Kumar (Department of Surgery and Obstetrics, Faculty of Veterinary Science, Bangladesh Agricultural University) ;
  • Alam, Md. Golam Shahi (Department of Surgery and Obstetrics, Faculty of Veterinary Science, Bangladesh Agricultural University) ;
  • Bari, Farida Yeasmin (Department of Surgery and Obstetrics, Faculty of Veterinary Science, Bangladesh Agricultural University)
  • Received : 2012.01.12
  • Accepted : 2012.01.25
  • Published : 2012.03.31
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Abstract

A study was conducted on four crossbred bulls, used as artificial insemination (AI) sires, to correlate their semen quality with their non return rate (NRR). Semen was collected once a week via an artificial vagina, diluted in egg yolk-citrate and maintained at $+7^{\circ}C$ for three days. It was evaluated for sperm motility, viability, morphology immediately after collection and was examined daily for sperm motility, viability and morphology of acrosome, mid piece and tail for a total of three days. A total of 2016 cows were inseminated by two AI technicians. The proportions of sperm with normal heads were 83.4% (63.7~91.7%), the proportion of spermatozoa exhibiting normal morphology (acrosome, mid piece and tail), motility and viability were 89.2% (82.3~92.0%), 71.3% (61.7~75.0%) and 76.7% (65.7~85.0%), respectively in fresh ejaculates. Sperm motility and sperm viability was significantly ($p$ <0.05) lower in Holstein-Friesian ${\times}$ Local bull than in other bulls during all three days of storage. The overall NRR for four bulls was 82.7% (72.9-87.5%). Bulls with higher sperm motility, viability and normal morphology of spermatozoa of individual bull had significantly (each $p$ <0.05) higher NRR. The highest ($p$ <0.01) NRR (87.5%) was observed in a Red Chittagong bull whose semen qualities were significantly ($p$ <0.05) higher than Holstein-Friesian ${\times}$ Local bull (NNR 72.9%). The results of the present study concluded that NRR at 56 days post AI is related to parameters of semen quality. Therefore, semen evaluation may allow the discarding of bulls with poor fertility in an AI program.

Keywords

References

  1. Ahmed Z and Islam TS. 1987. Cattle breeding programme through artificial insemination in Bangladesh. Artificial insemination extension project, Central Cattle Breeding Station, Department of Livestock Services, Savar, Dhaka, Bangladesh, pp. 1-68.
  2. Alam MGS, Yeashmin S, Bari FY and Mishra B. 2005. The effect of duration of preservation of the quality of chilled bull semen. The Bangladesh Veterinarian 22:16-22.
  3. Al-Makhzoomi A, Lundeheim N, Haard M and Rodriguez-Martinez H. 2008. Sperm morphology and fertility of progenytested AI dairy bulls in Sweden. Theriogenology 70:682- 691.
  4. Amann RP and Hammerstedt RH. 2002. Detection of differences in fertility. Journal of Andrology 23:317-325.
  5. Bane A. 1952. A study on the technique of hemocytometric determination of sperm motility and sperm concentration in bull semen. Cornell Veterinarian 42:518-531.
  6. Barth AD and Oko RJ. 1989. Abnormal Morphology of Bovine Spermatozoa. Ames, Iowa State University Press. IA, USA, pp. 285.
  7. Bhuiyan MMU and Shamsuddin M. 1998. Bull sperm morphology in context of ambient temperature during semen collection. Bang. Vet. J. 32:33-39.
  8. Bhuiyan MMU, Shamsuddin M and Galloway D. 1999. Identification of poor fertility bulls in AI programmes. The Bangladesh Veterinarian 16:67-71.
  9. Card C. 2005. Cellular associations and the differential spermiogram: making sense of stallion spermatozoal morphology. Theriogenology 64:558-567.
  10. Decuadro-Hansen G, Camus A and Delhomme G. 2002. Relationship between flow cytometric assessment of bull semen viability and the non return rates. First field trial in France. In: Proceedings of the 14th Meeting of European AI Vets. Lugo, Spain: University of Lugo. 25:105-108.
  11. Evans G and Maxwell WMC. 1990. Salamon's Artificials Insemination of Sheep and Goats, Butterworth, Sydney, pp. 85-121.
  12. Foote RH. 1969. Research techniques to study reproductive physiology in the male. Techniques and Proceduresi in Animal Science Research. American Society of Animal Science, Champaign 111:81.
  13. Freneau GE, Chenoweth PJ, Ellis R and Rupp G. 2009. Sperm morphology of beef bulls evaluated by two different methods.Anim. Reprod. Sci. 30:119-125.
  14. Hallap T, Jaakma U and Rodriguez-Martinez H. 2006. Changes in semen quality in Estonian Holstein AI bulls at 3, 5 and 7 years of age. Reprod. Dom. Anim. 41:214-218.
  15. Helbig L, Woodbury MR, Haigh JC and Barth AD. 2006. The onset of puberty in North American bison (Bison bison) bulls. Anim. Reprod. Sci. 97:12-24.
  16. Holt WV. 2000. Fundamental aspects of sperm cryobiology: the importance of species and individual differences. Theriogenology 59:47-58.
  17. Islam MH, Bhuiyan MMU and Alam MGS. 1999. Qualitative changes of frozen bull semen under field condition in Bangladesh. The Bangladesh Veterinarian 16:86-90.
  18. Jha PK. 2008. Improvement of keeping quality of chilled bull semen using ${\alpha}$-tocopherol as an antioxidant, MS Thesis, Department of Surgery and Obstetrics, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh.
  19. Munsi MN, Bhuiyan MMU, Majumder S and Alam MGS. 2007. Effects of exogenous glutathion on the quality of chilled bull semen. Reprod. Dom. Anim. 42:358-362.
  20. Reurink, Den Daas JHG and Wilmink JBM. 1990. Effects of AI sires and technicians on non return rates in the Netherlands. Livestock Prod. Sci. 26:107-118.
  21. Rodriguez-Martinez H. 2008. Estimation of fertility in breeding bulls. In: Proceeding 15th International Congress on Biotechnology in Animal Reproduction, Bangladesh Agricultural University, Mymensingh, 7-8 August, pp. 90-106.
  22. Sarder MJU. 2006. Evaluation of semen fertility of bulls by non return rate at 60 days of cows under artificial insemination programme in Bangladesh. Pakistan Journal of Biological Sciences 9:2207-2214.
  23. Shaha SP, Alam MGS, Khatun M and Ahmed JU. 2008. Breeding soundness of stud bulls. The Bangladesh Veterinarian 25:51-61.
  24. Shamsuddin M and Chanda PK. 1998. Effects of diluents and dilution rates on the preserved buck semen. 4th Asian Symposium on Animal Biotechnology, Kamiina, Japan, 7-9 October, pp. 177-181.
  25. Siddiqui MAR, Bhattacharjee J, Das ZC, Islam MM, Islam MA, Haque MA, Parrish JJ and Shamsuddin M. 2007. Crossbred Bull selection for bigger scrotum and shorter age at puberty with potentials for better quality semen. Reprod. Dom. Anim. 43:74-79.
  26. Stevenson JS, call EP, Scoby RK and Phatak AP. 1990: Double insemination and gonadotrophin releasing hormone treatment of repeat breeding dairy cattle. Journal of Dairy Science 73: 1766-1772.
  27. Sugulle AH, Bhuiyan MMU and Shamsuddin M. 2006. Breeding soundness of bulls and the quality of their frozen semen used in cattle artificial insemination in Bangladesh. Livestock Research for Rural Development 18:18-24.
  28. Vishwanath R, 2003: Artificial insemination: the state of the art. Theriogenology 59:571-584.