Journal of Korean society of Dental Hygiene (한국치위생학회지)

Korean Society of Dental Hygiene (한국치위생학회)
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Comparative study on survival rate of human gingival fibroblasts stored in different storage media

수종의 저장용액에서의 치은섬유모세포 생존율의 비교연구

  • Lee, Hee Su (Department of Anatomy and Histology, College of Dentistry Gangneung-Wonju National University) ;
  • Lim, You Sun (Department of Anatomy and Histology, College of Dentistry Gangneung-Wonju National University)
  • 이희수 (강릉원주대학교 치과대학 해부학 및 조직학 교실) ;
  • 임유선 (강릉원주대학교 치과대학 해부학 및 조직학 교실)
  • Received : 2012.06.05
  • Accepted : 2012.08.20
  • Published : 2012.08.31
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Abstract

Objectives : To Compare the degree of survival rate of gingival fibroblasts, which is concerned with teeth adherence based on the type of avulsed tooth's storage solution. Methods : Different media gingival fibroblasts were stored in Dulbecco's modified Eagle's medium(DMEM), Hank's balanced salt solution(HBSS), milk, saline, and green tea in for 1, 2, 3 hours. And, MTT assay was conducted to compare survival rate of human gingival fibroblasts. Results : 1. The survival rate of gingival fibroblasts in DMEM and HBSS was higher than thoes in other storage media( Milk> Saline> Green tea). 2. The survival rate of gingival fibroblasts in milk, saline and green tea decreased as time passed. 3. Because of low osmotic pressure, green tea showed decrease of survival rate of gingival fibroblasts. Conclusion : DMEM and HBSS were the most effective storage media for gingival fibroblast. Among milk, saline, green tea, milk is most effective storage media for keeping gingival fibroblasts. Milk is recommended for storage media of avulsed tooth for keeping viability of cells.

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