Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effect of Soybeans, Chungkukjang, and Doenjang on Blood Glucose and Serum Lipid Profile in Streptozotocin-induced Diabetic Rats

대두, 청국장 및 된장 분말의 급여가 Streptozotocin 유발 당뇨쥐의 혈당 및 혈청지질 성상에 미치는 영향

  • Received : 2012.03.05
  • Accepted : 2012.04.03
  • Published : 2012.05.31
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Abstract

This study investigated the effect of soybeans, $chungkukjang$, and $doenjang$ on blood glucose and serum lipid profile in streptozotocin (STZ)-induced diabetic rats. Male Sprague-Dawley rats weighing 205 g were divided into non-diabetic and diabetic groups. The diabetic groups were further subdivided into four experimental groups: a normal group (N), a diabetic control group (STZ-C), a diabetic group fed autoclaved soybean powder (STZ-S), a diabetic group fed $chungkukjang$ powder (STZ-CKJ) and a diabetic group fed $deonjang$ powder (STZ-DJ). Food and water intakes were higher in the diabetic groups than in the N group. The body weight gain and food efficiency ratios were higher in the STZ-S, STZ-CKJ, and STZ-DJ groups than in the STZ-C group, but created no significant changes between the diabetic groups on food intake and body weight gain. The whole blood level at 4 weeks of the STZ-CKJ group was significantly lower than the STZ-C group. Serum glucose levels of the STZ-S, STZ-CKJ, and STZ-DJ groups were significantly lower than the STZ-C group, but there was no significant change in serum insulin levels. The AST and ALP activities in serum were markedly higher in the STZ-C group, but these decreases in relation to diabetes increased in the STZ-S, STZ-CKJ, and STZ-DJ groups. The level of serum triglycerides was lower in the STZ-CKJ and STZ-DJ groups than in the STZ-C group, whereas level of serum HDL-cholesterol was higher in the STZ-CKJ and STZ-DJ groups. Levels of total serum and LDL-cholesterol were higher in the diabetic groups compared with the N group, but significantly decreased in the STZ-S, STZ-CKJ, and STZ-DJ groups compared to the STZ-C group. These results indicate that dietary supplements of soybean, $chungkukjang$ and $doenjang$ may improve blood glucose and lipid metabolism and help prevent or attenuate the progression of diabetes in STZ-induced diabetic rats.

본 연구는 동일 품종으로 제조한 증자대두 분말, $Bacillus$ $subtilis$ DJI로 발효시켜 제조한 청국장과 된장 분말이 streptozotocin 유발 당뇨쥐의 혈당 및 혈청지질 성상에 미치는 영향을 살펴보고자 정상군(N), 당뇨 대조군(STZ-C), 당뇨 대두군(STZ-S), 당뇨 청국장군(STZ-CKJ) 및 당뇨 된장군(STZ-DJ)으로 5군으로 나누어 4주간 실시하였고, 혈당변화, 혈청 포도당 및 인슐린 농도, 혈청 지질농도 및 효소 활성 등을 측정하였다. 체중증가량은 대두, 청국장 및 된장 분말을 급여한 군들이 당뇨 대조군에 비하여 유의적 차이는 없었으나 다소 증가하였다. 식이섭취량과 수분섭취량은 모든 당뇨 유발군들이 정상군에 비하여 유의적으로 증가하였고, 식이효율은 대두, 청국장 및 된장 분말을 급여한 군들이 당뇨 대조군에 비하여 증가하는 경향이었으나 유의차는 없었다. 간과 신장의 무게는 당뇨 유발군들이 정상군에 비하여 유의적으로 증가하였고, 대두, 청국장 및 된장 분말의 급여로 다소 감소하는 경향이었으나 유의적 차이는 없었다. 혈당의 변화는 대두, 청국장 및 된장 분말의 4주간 급여로 혈당 수준이 감소하는 경향이었고, 특히 4주째에 당뇨 청국장군은 당뇨 대조군에 비하여 유의적으로 낮은 혈당 수준을 나타내었다. 시험 종료 후 혈청 포도당 농도는 대두, 청국장 및 된장분말의 급여로 당뇨 대조군에 비하여 모두 유의적으로 낮게 나타났고, 혈청 인슐린 농도는 당뇨 유발군들 간에는 유의적인 차이가 없었다. 혈청 중 AST 및 ALP 활성은 당뇨 대조군에 비하여 대두, 청국장 및 된장 분말을 급여한 군들이 모두 유의하게 저하되었고, 특히 당뇨 청국장군의 ALP 활성은 정상군과 비슷한 경향이었다. 당뇨 유발로 증가된 혈청 중 콜레스테롤 및 LDL-콜레스테롤 함량은 대두, 청국장 및 된장 분말의 급여로 모두 당뇨 대조군에 비하여 유의하게 감소되었다. 혈청 중 중성지방 함량은 당뇨 유발군들이 정상군에 비하여 유의하게 증가되었고, 당뇨 청국장군과 당뇨 된장군은 당뇨 대조군에 비하여 유의하게 감소되었다. 혈청 중 HDL-콜레스테롤 함량은 당뇨 대조군이 정상군보다 낮은 경향이었으나 유의적인 차이는 없었고, 청국장 및 된장 분말급여 시 당뇨 대조군에 비하여 유의하게 증가하였으며, 정상군보다도 높은 경향이었으나 유의차는 없었다. 이상의 결과 증자대두 분말, $B.$ $subtilis$ DJI로 발효시켜 제조한 청국장과 된장 분말의 급여는 STZ로 유발된 당뇨쥐의 혈당 강하효과 및 당뇨 시 혈청 지질대사 개선 등 당뇨증세 완화에 긍정적으로 작용할 것으로 사료되며, 혈당 강하효과는 청국장이, 혈청 지질대사 개선효과는 청국장과 된장이 그 효과가 더 큰 것으로 나타났다.

Keywords

References

  1. Lee SJ, Park JY, Nam CM, Jee SH. 2008. The prevalence estimation of metabolic syndrome and it's related factors based on data from general health medical examination: a multi-center study. J Korean Soc Health Information Health Statistics 33: 119-133.
  2. Park SW, Kim DJ, Min KW, Baik SH, Choi KM, Park JB. 2007. Current status of diabetes management in Korea using national health insurance database. J Korean Diabetes Assoc 31: 362-367. https://doi.org/10.4093/jkda.2007.31.4.362
  3. Coulston AM, Hollenbeck CB. 1988. Source and amount of dietary carbohydrate in patients with noninsulin-dependent diabetes mellitus. Top Clin Nutr 3: 17-24.
  4. Williams SR. 1993. Nutrition and diet therapy. Mosby, St. Louis, MO, USA. p 635-660.
  5. Dey D, Mukherjee M, Basu D, Datta M, Roy SS, Bandyopadhyay A, Bhattacharya S. 2005. Inhibition of insulin receptor gene expression and insulin signaling by fatty acid: interplay of PKC isoforms therin. Cell Physiol biochem 16: 217-228. https://doi.org/10.1159/000089847
  6. Dillman WH. 1980. Diabetes mellitus induces changes in cardiac myosin of the rat. Diabetes 29: 579-582. https://doi.org/10.2337/diab.29.7.579
  7. Bailey CJ. 1999. Insulin resistance and antidiabetic drugs. Biochem Pharmacol 58: 1511-1520. https://doi.org/10.1016/S0006-2952(99)00191-4
  8. Kim JW, Cha JY, Heo JS, Jin HJ, Cho YS. 2008. Hypoglycemic effect of Chlorella sp. CMS-1 water extract on streptozotocin- induced diabetic rats. Journal of Life Science 18: 1584-1591. https://doi.org/10.5352/JLS.2008.18.11.1584
  9. Lim SJ, Park HJ. 2000. The effect of BuOH fraction of Polygonatum odoratum with selenium on blood glucose level and lipid peroxidation in streptozotocin induced diabetic rats. Korean J Nutr 33: 703-711.
  10. Setchell KD, Brown NM, Lydeking-Olsen E. 2002. Theclinical importance of the metabolite equol－a clue to the effectiveness of soy and its isoflavones. J Nutr 132: 3577-3584. https://doi.org/10.1093/jn/132.12.3577
  11. Messina M, Persky V, Setchell KDR, Barnes S. 1994. Soy intake and cancer risk: a review of the in vitro and in vivo data. Nutr Cancer 21: 113-131. https://doi.org/10.1080/01635589409514310
  12. Ruiz-Larrea MB, Mohan AR, Paganga G, Miller NJ, Bolwell GP, Rice-Evans CA. 1997. Antioxidant activity of phytoestrogenic isoflavones. Free Rad Res 26: 63-70. https://doi.org/10.3109/10715769709097785
  13. Park GS. 2004. Cookwise approach of slow food: focused on traditional fermented sauces. Korean J Soc Food Cookery Sci 20: 317-334.
  14. Ryu SH. 2002. Studies on antioxidative effects and antioxidative components of soybean and chongkukjang. PhD Dissertation. Inje University, Gyengnam, Korea. p 23-122.
  15. Koh JB. 1998. Effects of raw soy flour (yellow and black) on serum glucose and lipid concentrations in streptozotocin- induced diabetic rats. J Korean Soc Food Sci Nutr 27: 313-318.
  16. Kim MH, Kim HY, Kim WK, Kim JY, Kim SH. 2001. Effects of soy oligosaccharides on blood glucose and lipid metabolism in streptozotocin-induced biabetic rats. Korean J Nutr 34: 3-13.
  17. Nuttall FQ. 1993. Dietary fiber in the management of diabetes. Diabetes 42: 503-508. https://doi.org/10.2337/diabetes.42.4.503
  18. Wiseman H. 2000. The therapeutic potential of phytoestrogens. Expert Opin Investig Drugs Aug 9: 1829-1840. https://doi.org/10.1517/13543784.9.8.1829
  19. Kakade ML, Hoffa DE, Liener IE. 1973. Contribution of trypsin inhibitors to the deleterius effects of unheated soybeans fed to rats. J Nutr 103: 1772-1778. https://doi.org/10.1093/jn/103.12.1772
  20. Nakamura N, Yoshida T. 1980. Effect of trypsin inhibitor on blood sugar, insulin and glucagon levels in normal and streptozotocin rats. J Kyoto Pref Univ Med 89: 465.
  21. Kim SH, Jung SH, Kim IH, Lee YS, Lee JM, Kim JG, Lee MC, Choi MJ, Kim DJ. 2008. The effects of Chungkookjang biopolymer on blood glucose and serum lipid lowering in streptozotocin-induced diabetic rats. J Korean Soc Food Sci Nutr 37: 35-41. https://doi.org/10.3746/jkfn.2008.37.1.35
  22. Kim HJ, Kim YC. 2006. Antidiabetic and antioxidant effects of chunggugjang powder in streptozotocin-induced diabetic rats. J Environ Toxicol 21: 139-146.
  23. Kwon TW. 2003. The effect of physiological functionality of soybean on prevention and treating chronic diseases and its clinical application. National Research Foundation of Korea R01-2000-000-00187-0.
  24. Chang M, Kim IC, Chang HC. 2010. Effect of solar salt on the quality characteristics of Doenjang. J Korean Soc Food Sci Nutr 39: 116-124. https://doi.org/10.3746/jkfn.2010.39.1.116
  25. Min HK, Kim HJ, Chang HC. 2008. Growth-inhibitory effect of the extract of porphyran-chungkukjang on cancer cell. J Korean Soc Food Sci Nutr 37: 826-833. https://doi.org/10.3746/jkfn.2008.37.7.826
  26. Chang M, Chang HC. 2007. Characteristics of bacterial-koji and deoenjang (soybean paste) made by using B. subtilis DJI. Kor J Microbiol Biotechnol 35: 325-333.
  27. Kim AR, Lee JJ, Chang HC, Lee MY. 2009. Antioxidative effects of chungkukjang fermented using Bacillus subilis DJI in rats fed a high cholesterol diet. J Korean Soc Food Sci Nutr 38: 1699-1706. https://doi.org/10.3746/jkfn.2009.38.12.1699
  28. Lee JJ, Kim AR, Lee H, Kim CH, Chang HC, Lee MY. 2011. Effects of soybean, cheonggukjang and doenjang on serum cholesterol level and weight reduction in rats fed a highfat/ high-cholesterol diet. Korean J Food Preserv 18: 226-235. https://doi.org/10.11002/kjfp.2011.18.2.226
  29. Friedwald WT, Levy RL, Fredrickson DS. 1972. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 18: 499-502.
  30. Beppu H, Maruta K, Kurner T, Kolb H. 1987. Diabetogenic action of streptozotocin: essential role of membrane permeability. Acta Endocrinol 114: 90-95.
  31. Furuse M, Kimura C, Mabayo RT, Takahashi H, Okumura J. 1993. Dietary sorbose prevents and improves hyperglycemia in genetically diabetic mice. J Nutr 123: 59-65. https://doi.org/10.1093/jn/123.1.59
  32. Malabu UH, Dryden S, Mccarthy HD, Kilpatrick A, Williams G. 1994. Effect of chronic vanadate administration in the STZ-induced diabetic rats: the antihyperglycemic action of vanadate in attributable entirely to its suppression of feeding. Diabetes 43: 9-15. https://doi.org/10.2337/diabetes.43.1.9
  33. O'Meara NM, Devery Ra, Owens D, Collins PB, Johnson AH, Tomkin GH. 1990. Cholesterol metabolism in alloxan-induced diabetic rabbits. Diabetes 39: 626-633. https://doi.org/10.2337/diabetes.39.5.626
  34. Smith OLK, Wong CY, Gelfand RA. 1989. Skeletal muscle proteolysis in rat with acute strptozotocin-induced diabetes. Diabetes 38: 1117-1122. https://doi.org/10.2337/diabetes.38.9.1117
  35. Goldberg RB. 1981. Lipid disorders in diabetes. Diabetes Care 4: 561-572. https://doi.org/10.2337/diacare.4.5.561
  36. Steer KA, Sochor M, McLean P. 1985. Renal hypertrophy in experimental diabetes changes in pentose phosphate pathway activity. Diabetes 34: 485-490. https://doi.org/10.2337/diabetes.34.5.485
  37. Dai S, Thompson KH, McNeill JH. 1994. One-year treatment of streptozotocin-induced diabetic rats with vanadyl sulphate. Pharmacol Toxicol 74: 101-109. https://doi.org/10.1111/j.1600-0773.1994.tb01083.x
  38. Park SA, Kim MJ, Jang JY, Choi MS, Yeo JY, Lee MK. 2006. Effect of genistein and daidzein on antioxidant defense system in C57BL/KsJ-db/db mice. J Korean Soc Food Sci Nutr 35: 1159-1165. https://doi.org/10.3746/jkfn.2006.35.9.1159
  39. Matkovics B, Kotorman M, Varga IS, Hai DQ, Varga C. 1998. Oxidative stress in experimental diabetes induced by streptozotocin. Acta Physiol Hung 85: 29-38.
  40. Park HR, Cho JS. 2007. Effects of a natural medicinal multiplant extract on blood glucose, insulin levels, and serum malondialdehyde concentration in streptozotocin-induced diabetic rats. J East Asian Soc Dietary Life 17: 205-212.
  41. Davis A, Christiansen M, Horowitz JF, Klein S, Hellerstein MK, Ostlund RE. 2000. Effects of pinitol treatment on insulin action in subjects with insulin resistance. Diabetes Care Jul 23: 1000-1005. https://doi.org/10.2337/diacare.23.7.1000
  42. Lee YL, Kim SH, Choung NH, Yim MH. 1992. A study on the production of viscous substance during Chungkookjang fermentation. J Korean Soc Agric Chem Biotechnol 35: 202-209.
  43. Kang SA, Hong KH, Kim SH, Jang KH, Kim CH, Choue RW. 2002. Effects of dietary levan on adiposity, serum leptin and UCP expression in obese rats fed high fat diet. Korean J Nutr 35: 903-911.
  44. Jung SH, Park YJ. 2001. The effects of isolated soyprotein and salt restriction on serum lipid and kidney function of streptozotocin-induced diabetic rats. J East Asian Soc Dietary Life 11: 368-378.
  45. Ali AA, Velasquez MT, Hansen CT, Mohamed AI, Bhathena SJ. 2005. Modulation of carbohydrate metabolism and peptide hormones by soybean isoflavones and probiotics in obesity and diabetes. J Nutr Biochem 16: 693-699. https://doi.org/10.1016/j.jnutbio.2005.03.011
  46. Lee JS. 2006. Effects of soy protein and genistein on blood glucose, antioxidant enzyme activities, and lipid profile in streptozotocin-induced diabetic rats. Life Sci 79: 1578-1584. https://doi.org/10.1016/j.lfs.2006.06.030
  47. Yamamoto Y, Takahashi Y, Kawano M, Iizuka M, Matsumoto T, Saeki S, Yamaguchi H. 1999. In vitro digestibility and fermentability of levan and its hypocholesterolemic effects in rats. J Nutr Biochem 10: 13-18. https://doi.org/10.1016/S0955-2863(98)00077-1
  48. Lavigne C, Marette A, Jacques H. 2000. Cod and soy proteins compared with casein improve glucose tolerance and insulin sensitivity in rats. Am J Physiol Endocrinol Metab 278: E491-500. https://doi.org/10.1152/ajpendo.2000.278.3.E491
  49. Choi JW, Sohn KH, Kim SH. 1991. Effects of nicotinamide on the serum lipid composition in streptozotocin-induced diabetic rats. J Korean Soc Food Nutr 20: 306-311.
  50. Lim AK, Jung HK, Hong JH, Oh JS, Kwak JH, Kim YH, Kim DI. 2008. Effects of the soybean powder with rich aglycone isoflavone on lipid metabolism and antioxidative activities in hyperlipidemic rats. J Korean Soc Food Sci Nutr 37: 302-308. https://doi.org/10.3746/jkfn.2008.37.3.302
  51. Lee JJ, Kim AR, Lee H, Kim CH, Chang HC, Lee MY. 2010. Effects of powders of soybean and doenjang on cholesterol level and antioxidant activities in rats fed with a high cholesterol diet. Korean Journal of Life Science 20: 1134-1142. https://doi.org/10.5352/JLS.2010.20.7.1134
  52. Niall MG, Rosaleen AM, Daphne O, Patrick BC, Alan HJ, Gerald HT. 1990. Cholesterol metabolism in alloxan-induced diabetic rabbits. Diabetes 9: 626-631.
  53. Siegel RD, Cuples A, Schaefer EJ, Wilson PWF. 1996. Lipoproteins, apolipoproteins, and low-density lipoprotein size among diabetics in the framingham offspring study. Metabolism 45: 1267-1272. https://doi.org/10.1016/S0026-0495(96)90246-2
  54. Kim SH, Kang JS, Lee SJ, Chung YJ. 2008. Antidiabetic effect of Korean red ginseng by puffing process in streptozotocin- induced diabetic rats. J Korean Soc Food Sci Nutr 37: 701-707. https://doi.org/10.3746/jkfn.2008.37.6.701
  55. O'Meara NM, Devery RA, Owens D, Collins PB, Johnson AH, Tomkin GH. 1990. Cholesterol metabolism in alloxan induced diabetic rabbits. Diabetes 39: 629-633.
  56. Durrington PN, Stephens WP. 1980. The effects of treatment with insulin on serum high-density-lipoprotein cholesterol in rats with streptozotocin-induced diabetes. Clin Sci 59: 71-74. https://doi.org/10.1042/cs0590071
  57. Kim JS. 1996. Current research trends on bioactive function of soybean. Korea Soybean Society 13: 17-24.
  58. Kirk EA, Sutherland P, Wang SA. 1998. Dietary isoflavones reduce plasma cholesterol and atherosclerosis in C57BL/ 6mice but not LDL receptor-deficient mice. J Nutr 128: 954-959. https://doi.org/10.1093/jn/128.6.954
  59. Potter SM. 1995. Overview of proposed mechanisms for the hypocholesterolemic effect of soy. J Nutr 125: 606S-611S.

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