Preparation and Characterizations of Complex Composed of ${\beta}$-Cyclodextrin Polymer/Cinnamic Acid

베타-사이클로 덱스트린 중합체/신남산 복합체의 제조 및 특성 연구

  • Mok, Eun Young (College of Biomedical Science and Institute of Bioscience and Biotechnology, Kangwon National University) ;
  • Cha, Hyun Ju (College of Biomedical Science and Institute of Bioscience and Biotechnology, Kangwon National University) ;
  • Kim, Jin-Chul (College of Biomedical Science and Institute of Bioscience and Biotechnology, Kangwon National University)
  • 목은영 (강원대학교 의생명과학대학) ;
  • 차현주 (강원대학교 의생명과학대학) ;
  • 김진철 (강원대학교 의생명과학대학)
  • Published : 2012.10.10

Abstract

$\beta$-cyclodextrin ($\beta$-CD) polymers were prepared in a strong alkali condition solution (NaOH solution 30% (w/v)) using epichlorohydrin (EPI) as a cross-linker, and the molar ratio of EPI to $\beta$-CD was 10 : 1. The $\beta$-CD content in $\beta$-CD polymers is about 52%. In order to get the photo-responsible and pH-responsible, cinnamic acid was added to be inserted into the cavities of $\beta$-CD due to the hydrophobic interaction. The complex formation was confirmed using transmission electron microscope. The dimerization degree of complexes increased under UV irradiation at $\lambda$ = 365 nm but decreased under the UV irradiation at $\lambda$ = 254 nm. Dynamic light scattering analysis of particle sizes showed that the sizes of complexes did not change with different UV wavelength. Moreover, the complexes were pH-responsible because of the carboxyl group of cinnamic acid, but the size and zeta potential of the complex did not change in strong acid and alkali conditions.

베타-사이클로덱스트린($\beta$-cyclodextrin, $\beta$-CD) 중합체(polymer)는 가교제인 epichlorohydrin (EPI)과 $\beta$-CD의 몰 비가 10 : 1으로, 강한 염기조건에서 합성하였다. 합성한 $\beta$-CD 중합체 내의 $\beta$-CD contents는 52%였다. 광, pH반응성 복합체를 제조하기 위해 신남산을 첨가하였고 첨가된 신남산은 소수성 상호작용에 의해 $\beta$-CD 공동에 포접되었다. 형성된 $\beta$-CD 중합체와 신남산 복합체를 투과전자현미경을 이용하여 관찰하였을 때 복합체의 형상을 관찰하였다. 광 조사에 따른 이량화도는 $\lambda$ = 365 nm의 UV조사 시 증가하였으며 $\lambda$ = 254 nm의 UV조사 시 감소하였다. 또한 동적 광 산란(dynamic light scattering)장치를 이용하여 측정한 복합체의 크기는 광 조사 유무에 따라 큰 변화가 측정 되지 않았고, pH 반응성을 관찰한 실험에서도 복합체의 크기와 제타 전위(zeta potential) 모두 pH에 따른 변화가 나타나지 않았다.

Keywords

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