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추출조건에 따른 산수유 열매 추출물의 생리활성

Physiological Properties of $Corni$ $fructus$ Extracts Based on Their Extraction Condition

  • 투고 : 2011.11.22
  • 심사 : 2012.03.16
  • 발행 : 2012.04.30

초록

본 연구에서는 산수유 열매 분말을 추출비율과 추출용매를 각각 달리하여 추출물의 생리활성을 측정 비교하였다. DPPH radical 소거능 측정 결과, 90% 에탄올 추출물이 97.20%로 가장 높았으며, 이는 표준물질인 L-ascorbate의 활성보다 높은 수치였다. 총 폴리페놀 함량은 25 mL/g>50 mL/g>100 mL/g의 순으로 추출물의 농도에 따라 페놀 함량이 유의적으로 높았으며, 추출용매에 따라 30% 에탄올>60% 에탄올>물>90% 에탄올의 순으로 함량이 측정되었다. Superoxide anion radical 소거능의 경우 추출비율에 따른 차이가 다소 있었으나, 모든 추출물들이 27.70~57.44%로 다소 낮은 경향을 보였다. Tyrosinase 저해 효과 측정에서는 추출물의 농도가 높을수록 효과가 우수하였다. 대부분의 추출물들이 63.44%~88.71%로 특정 추출조건에서 그 효과가 높게 나타남에 따라 산수유 열매가 미백효과에 탁월할 것으로 짐작할 수 있다. ACE 활성 저해 측정결과 모든 추출물이 20% 미만의 수치로 활성 저해력이 낮게 나타났다. 아질산염 소거에서는 pH가 낮을수록 소거력이 우수하였다(p<0.05). 산수유 열매의 생리활성이 우수한 것으로 나타남에 따라 산수유의 최적의 추출조건을 탐색하고 이를 바탕으로 하여 산수유가 천연식품으로서 기능적으로 우수한 다양한 제품의 개발 가능성을 기대할 수 있을 것이다.

Physiological properties of $Corni$ $fructus$ extracts were investigated. The DPPH radical scavenging activity of 90% ethanol extracts had shown 97.20% at 25 mL/g, which was highest in the all extracts. Total polyphenol content of 30% ethanol extracts had shown 140.01mg% at 25 mL/g, which was highest in the all extracts. The polyphenol contents increased as extract concentration rose (25 mL/g>50 mL/g>100 mL/g). Tyrosinase inhibitory effects of all samples were 23.96~88.71%, expecially, the highest at 60% ethanol extracts of 25 mL/g. Nitrite-scavenging abilities under acidic conditions (pH 1.2) were the most effective in all extracts. The results would be useful for understanding of the physiological properties of $Corni$ $fructus$ extracts.

키워드

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