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Optimum Extraction Conditions and Anticancer Effect of Functional Polysaccharide from Mycelia of Grifola frondosa

잎새버섯(Grifola frondosa) 균사체의 기능성 다당류 최적 추출방법 및 항암효과

  • Park, Chan-Ho (Dept. of Biology, College of Natural Science, Soonchunhyang University) ;
  • Lee, Gyeong-Min (Dept. of Biology, College of Natural Science, Soonchunhyang University) ;
  • Nam, Eun-Jeong (Dept. of Biology, College of Natural Science, Soonchunhyang University) ;
  • Yu, Yeon-Hee (Dept. of Biology, College of Natural Science, Soonchunhyang University) ;
  • Kim, Yong-Hyun (Dept. of Biology, College of Natural Science, Soonchunhyang University) ;
  • Kwon, Hyun-Jung (Dept. of Biology, College of Natural Science, Soonchunhyang University) ;
  • Yoon, Ok-Hyun (Dept. of Food Nutrition, Gimcheon University) ;
  • Han, Man-Deuk (Dept. of Biology, College of Natural Science, Soonchunhyang University)
  • 박찬호 (순천향대학교 생명과학과) ;
  • 이경민 (순천향대학교 생명과학과) ;
  • 남은정 (순천향대학교 생명과학과) ;
  • 유연희 (순천향대학교 생명과학과) ;
  • 김용현 (순천향대학교 생명과학과) ;
  • 권현정 (순천향대학교 생명과학과) ;
  • 윤옥현 (김천대학교 식품영양학과) ;
  • 한만덕 (순천향대학교 생명과학과)
  • Received : 2012.02.27
  • Accepted : 2012.03.14
  • Published : 2012.03.31

Abstract

Grifola frondosa has been used as an herbal medicine for the treatment of cancer, diabetes mellitus and high blood pressure. In this study, functional polysaccharide was obtained from Grifola frondosa using four different extraction methods: hot water(HwFP), homogenize(HgFP), acid(AcFP), and alkali(AlFP) extraction methods. The effects of these extracts on KB and HepG2 cell lines were then examined for any anti-cancer activity. Alkaline extraction produced a yield of 0.175% and the total sugar content of the extract was 54.97%. We were able to confirm that the polysaccharide extracts from the mushroom produce an anti-cancer effect. The cytotoxicity of AlFP and AcFP against HepG2 cells were 22.86% and 28.88%, respectively, and the cytotoxicity of AlFP against the KB cell lines was 47.76% at a concentration of 1,000 ${\mu}g/m{\ell}$. Therefore, these results suggest that the optimum method for extracting functional polysaccharides from G. frondosa is the alkali extraction method.

Keywords

References

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