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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Identification of interacting proteins of retinoid-related orphan nuclear receptor gamma in HepG2 cells

  • Huang, Ze-Min (Institute of Immunology, PLA, Third Military Medical University) ;
  • Wu, Jun (Chongqing Key Laboratory for Disease Proteomics) ;
  • Jia, Zheng-Cai (Institute of Immunology, PLA, Third Military Medical University) ;
  • Tian, Yi (Institute of Immunology, PLA, Third Military Medical University) ;
  • Tang, Jun (Department of Dermatology the 105th, Hospital of PLA) ;
  • Tang, Yan (Institute of Immunology, PLA, Third Military Medical University) ;
  • Wang, Ying (Chongqing Key Laboratory for Disease Proteomics) ;
  • Wu, Yu-Zhang (Institute of Immunology, PLA, Third Military Medical University) ;
  • Ni, Bing (Institute of Immunology, PLA, Third Military Medical University)
  • Received : 2011.11.21
  • Accepted : 2011.02.07
  • Published : 2012.06.30
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Abstract

The retinoid-related orphan nuclear receptor gamma ($ROR{\gamma}$) plays critical roles in regulation of development, immunity and metabolism. As transcription factor usually forms a protein complex to function, thus capturing and dissecting of the $ROR{\gamma}$ protein complex will be helpful for exploring the mechanisms underlying those functions. After construction of the recombinant tandem affinity purification (TAP) plasmid, pMSCVpuro $ROR{\gamma}$-CTAP(SG), the nuclear localization of $ROR{\gamma}$-CTAP(SG) fusion protein was verified. Following isolation of $ROR{\gamma}$ protein complex by TAP strategy, seven candidate interacting proteins were identified. Finally, the heat shock protein 90 (HSP90) and receptor-interacting protein 140 (RIP140) were confirmed to interplay with $ROR{\gamma}$ by co-immunoprecipitation. Interference of HSP90 or/and RIP140 genes resulted in dramatically decreased expression of CYP2C8 gene, the $ROR{\gamma}$ target gene. Data from this study demonstrate that HSP90 and RIP140 proteins interact with $ROR{\gamma}$ protein in a complex format and function as co-activators in the $ROR{\gamma}$-mediated regulatory processes of HepG2 cells.

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References

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