Food Science of Animal Resources (한국축산식품학회지)

Korean Society for Food Science of Animal Resources (한국축산식품학회)
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The Biological Effects of Bovine Lactoferrin on Inflammatory Cytokine Expression in the PMA Stimulated Cells

PMA로 자극되어진 세포에서 염증 Cytokine 발현에 미치는 Bovine Lactoferrin의 생물활성 영향

  • Chung, Sung-Hee (Department of Animal Biosystem Science, Chungnam National University) ;
  • Kang, Ho-Bum (Genomics Medical Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Jae-Wha (Genomics Medical Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Yoon, Sung-Sik (Division of Biological Science and Technology, Yonsei University) ;
  • Nam, Myoung-Soo (Department of Animal Biosystem Science, Chungnam National University)
  • 정승희 (충남대학교 농업생명과학대학 동물바이오시스템과학과) ;
  • 강호범 (한국생명공학연구원 유전체의학연구센터) ;
  • 김재화 (한국생명공학연구원 유전체의학연구센터) ;
  • 윤성식 (연세대학교 생명과학기술학부) ;
  • 남명수 (충남대학교 농업생명과학대학 동물바이오시스템과학과)
  • Received : 2012.01.30
  • Accepted : 2012.06.08
  • Published : 2012.06.30
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Abstract

Bovine lactoferrin is well known as biological activator in defense mechanism related some cells. In this study, we was investigated about the immune modulator as a role of lactoferrin through the transcriptional regulation of genes associated with hypersensitivity such as allergy, athma and inflammatory disease. Effects of inflammatory reaction of bovine lactoferrin was carried out by RT-PCR analysis from isolated total RNA treated with lactoferrin 0, 10, 50, 100, 500 ${\mu}g/mL$ and PMA 100 ng/mL. The expression of the TYROBP, PITPNA, IL-10, SLP1, DC-stamp and ICAM-1 mRNA were increased by synergy effect of bovine lactoferrin and PMA. The results of RT-PCR showed that bovine lactoferrin and PMA had an effect of immune modulator by enhancement of TYROBP, PITPNA, SLP1, DC-stamp, IL-10 and ICAM-1 gene transcription in U937, Mutz-3 and NK92 cells, respectively. Bovine lactoferrin showed a potential of biological function which could be used for industrial applications as a material of food and pharmaceutical.

유청단백질의 일종인 lactoferrin은 이미 많은 보고에 의해서 여러 가지 생리활성기능이 있는 것으로 밝혀지고 있는데, U937, NK92, 수지상세포의 분화된 상태인 mutz-3와 muDC를 이용하여 과민반응과 천식, 면역 관련 유전자의 발현에 미치는 영향을 조사 한 결과 의미 있는 결과를 도출하였다. Lactoferrin 단독 또는 면역증강물질인 PMA와 혼합하여 처리 한 경우 상승효과 작용으로 과민반응과 천식, 면역 관련 유전자의 발현을 강하게 유도하였다. 이는 유청단백질의 주요 성분 중 하나인 lactoferrin이 면역기전에 중요한 역할을 하고 있다는 결과로 사료된다.

Keywords

References

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