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Methanol Extract of Goat's-beard (Aruncus dioicus) Reduces Renal Injury by Inhibiting Apoptosis in a Rat Model of Ischemia-Reperfusion

  • Baek, Hae-Sook (Department of Biochemistry, School of Medicine, Catholic University of Daegu) ;
  • Lim, Sun-Ha (Department of Biochemistry, School of Medicine, Catholic University of Daegu) ;
  • Ahn, Ki-Sung (Internal Medicine, School of Medicine, Catholic University of Daegu) ;
  • Lee, Jong-Won (Department of Biochemistry, School of Medicine, Catholic University of Daegu)
  • Received : 2012.03.29
  • Accepted : 2012.06.18
  • Published : 2012.06.30

Abstract

Interruption or prolonged reduction and subsequent restoration of blood flow into the kidney triggers the generation of a burst of reactive oxygen species (ROS), leading to injury in the tubular epithelial cells. In this study, we determined whether methanol extract of goat's-beard (Aruncus dioicus) (extract) could prevent this ischemia/reperfusion injury. When in vitro radical scavenging activity of the extract was measured using a DPPH radical quenching assay, the extract displayed slightly lower activity than ascorbic acid. One hour after administration of the extract (400 mg/kg) by intraperitoneal injection in rats, renal ischemia/reperfusion injury was generated by clamping the left renal artery for forty minutes, followed by 24 hr restoration of blood circulation. Prior to clamping the left renal artery, the right renal artery was removed. Compared with the vehicle-treated group, pretreatment with the extract significantly reduced the tubular epithelial cell injury by 37% in the outer medulla region, and consequently reduced serum creatinine concentration by 39%. Reduction in the cell injury was mediated by attenuation of Bax/Bcl-2 ratio, inhibition of caspase-3 activation from procaspase-3, and subsequent reduction in the number of apoptotic cells. Thus, goat's-beard (Aruncus dioicus) might be developed as a prophylactic agent to prevent acute kidney injury.

Keywords

References

  1. Abuelo JG. 2007. Normotensive ischemic acute renal failure. N Engl J Med 357: 797-805. https://doi.org/10.1056/NEJMra064398
  2. Venkataraman R, Kellum JA. 2007. Defining acute renal failure: the RIFLE criteria. J Intensive Care Med 22: 187-193. https://doi.org/10.1177/0885066607299510
  3. Lameire N, Van Biesen W, Vanholder R. 2005. Acute renal failure. Lancet 365: 417-430. https://doi.org/10.1016/S0140-6736(05)17831-3
  4. Bonventre JW. 1993. Mechanisms of ischemic acute renal failure. Kidney Int 43: 1160-1178. https://doi.org/10.1038/ki.1993.163
  5. Nath KA, Norby SM. 2000. Reactive oxygen species and acute renal failure. Am J Med 109: 655-678.
  6. Chatterjee PK. 2007. Novel pharmacological approaches to the treatment of renal ischemia-reperfusion injury: a comprehensive review. Naunym-Schmiedeberg's Arch Pharmacol 376: 1-43. https://doi.org/10.1007/s00210-007-0183-5
  7. Pandanilam BJ. 2003. Cell death induced by acute renal injury: a perspective on the contributions of apoptosis and necrosis. Am J Physiol Renal Physiol 284: F608-F627. https://doi.org/10.1152/ajprenal.00284.2002
  8. Shin JW, Lee SI, Woo MH, Kim SD. 2008. Effect of ethanol extracts of goat's beard on streptozotocin induced diabetic symptoms and oxidative stress in rats. J East Asian Soc Dietary Life 18: 939-948.
  9. Lee SO, Lee HJ, Yu MH, Im HG, Lee IS. 2005. Total polyphenol contents and antioxidant activities of methanol extracts from vegetables produced in Ullung island. Korean J Food Sci Technol 37: 233-240.
  10. Han HS, Lee JW. 2011. Attenuation of brain injury by water extract of goat's beard (Aruncus dioicus) and its ethyl acetate fraction in a rat model of ischemia-reperfusion. J Food Sci Nutr 16: 217-223. https://doi.org/10.3746/jfn.2011.16.3.217
  11. Tapuria N, Kumar Y, Habib MM, Amara MA, Seifalian AM, Davidson BR. 2008. Remote ischemic preconditioning: a novel protective method from ischemia reperfusion injury-a review. J Surg Res 150: 304-330. https://doi.org/10.1016/j.jss.2007.12.747
  12. Kim KH, Lee JW. 2010. Methanol extract of Cassia mimosoides var. nomame and its ethyl acetate fraction attenuate brain damage by inhibition of apoptosis in a rat model of ischemia-reperfusion. J Food Sci Nutr 15: 255-261. https://doi.org/10.3746/jfn.2010.15.4.255
  13. Jung HJ, Kim MJ, Lim SH, Park JH, Lee HK, Ahn KS, Lee JW. 2009. The effect of extract of Paeonia lactiflora on the improvement of ischemic acute renal failure. Korean J Nephrol 28: 180-189.
  14. Kuriakose GC, Kurup MG. 2010. Effects of Aulosura fertilisima against cisplatin-induced nephrotoxicity and oxidative stress in rats. Ren Fail 32: 224-233. https://doi.org/10.3109/08860220903541143
  15. Lim SH, Song KS, Lee JW. 2010. Butyrate and propionate, short chain fatty acids, attenuate myocardial damages by inhibition of apoptosis in a rat model of ischemiareperfusion. J Korean Soc Appl Biol Chem 53: 570-577. https://doi.org/10.3839/jksabc.2010.088
  16. Kelly KJ, Williams Jr WW, Colvin RB, Meehan SM, Springer TA, Gutierrez-Ramos JC, Bonventre JV. 1996. Intracellular adhesion molecule-1-deficient mice are protected against ischemic renal injury. J Clin Invest 97: 1056-1063. https://doi.org/10.1172/JCI118498
  17. Korsmeyer SJ. 1999. BCL-2 gene family and the regulation of programmed cell death. Cancer Res 59: 1693s-1799s.
  18. Jablonski P, Howden BO, Rae DA, Birrell CS, Marshall VC, Tange J. 1983. An experimental model for assessment of renal recovery from warm ischemia. Transplantation 35: 198-204. https://doi.org/10.1097/00007890-198303000-00002
  19. Heyman SN, Rosenberger C, Rosen S. 2010. Experimental ischemia-reperfusion: biases and myths-the proximal vs. distal hypoxic tubular injury debate revisited. Kidney Int 77: 9-16. https://doi.org/10.1038/ki.2009.347
  20. Erdogan H, Fadillioglu E, Yagmurca M, Ucar M, Irmak MK. 2006. Protein oxidation and lipid peroxidation after renal ischemia-reperfusion injury: protective effects of erdosteine and N-acetylcysteine. Urol Res 34: 41-46. https://doi.org/10.1007/s00240-005-0031-3
  21. Stevens LA, Levey AS. 2005. Measurement of kidney function. Med Clin N Am 89: 457-473. https://doi.org/10.1016/j.mcna.2004.11.009
  22. Kirkby K, Baylis C, Agarwal A, Croker B, Archer L, Adin C. 2007. Intravenous bilirubin provides incomplete protection against renal ischemia-reperfusion injury in vivo. Am J Physiol Renal Physiol 292: F888-F894. https://doi.org/10.1152/ajprenal.00064.2006
  23. Molyneux P. 2004. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J Sci Technol 26: 211-219.
  24. Korkmaz A, Kolankaya D. 2009. The protective effects of ascorbic acid against renal ischemia-reperfusion injury in male rats. Ren Fail 31: 36-43. https://doi.org/10.1080/08860220802546271
  25. Leelavinothan P, Kalist S. 2011. Beneficial effect of hesperetin on cadmium induced oxidative stress in rats: an in vivo and in vitro study. Eur Rev Med Pharmacol Sci 15: 992-1002.
  26. Xu R, Shang N, Li P. 2011. In vitro and in vivo antioxidant activity of exopolysaccharide fractions from Bifidobacterium animalis RH. Anaerobe 17: 226-231. https://doi.org/10.1016/j.anaerobe.2011.07.010
  27. Wagner M, Cadetg P, Ruf R, Mazzuchelli L, Ferrari P, Redaelli CA. 2003. Heme oxygenase-1 attenuates ischemia/ reperfusion-induced apoptosis and improves survival in rat renal allografts. Kid Int 63: 1564-1573. https://doi.org/10.1046/j.1523-1755.2003.00897.x
  28. Havasi A, Borkan SC. 2011. Apoptosis and acute kidney injury. Kid Int 80: 29-40. https://doi.org/10.1038/ki.2011.120
  29. Chien CT, Chang TC, Tsai CY, Shyue SK, Lai MK. 2005. Adenovirus-mediated Bcl-2 gene transfer inhibits renal ischemia/ reperfusion induced tubular oxidative stress and apoptosis. Am J Transplantation 5: 1194-1203. https://doi.org/10.1111/j.1600-6143.2005.00826.x
  30. Coleman MD, Shaefi S, Sladen RN. 2011. Preventing acute kidney injury after cardiac surgery. Curr Opin Anesthesiol 24: 70-76. https://doi.org/10.1097/ACO.0b013e3283422ebc
  31. Patel NN, Rogers CA, Angelini GD, Murphy GJ. 2011. Pharmacological therapies for the prevention of acute kidney injury following cardiac surgery: a systematic review. Heart Fail Rev 16: 553-567. https://doi.org/10.1007/s10741-011-9235-5

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