Preventive Nutrition and Food Science

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지
DOI QR코드

DOI QR Code

Antioxidant Activity and Protection from DNA Damage by Water Extract from Pine (Pinus densiflora) Bark

  • Jiang, Yunyao (Department of Medical Biotechnology, College of Biomedical Science, Kangwon National University) ;
  • Han, Woong (Department of Medical Biotechnology, College of Biomedical Science, Kangwon National University) ;
  • Shen, Ting (Department of Medical Biotechnology, College of Biomedical Science, Kangwon National University) ;
  • Wang, Myeong-Hyeon (Department of Medical Biotechnology, College of Biomedical Science, Kangwon National University)
  • Received : 2011.12.08
  • Accepted : 2012.05.25
  • Published : 2012.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Water extract from Pinus densiflora (WPD) was investigated for its antioxidant activity and its ability to provide protection from DNA damage. A series of antioxidant assays, including a 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical-scavenging assay, a reducing power assay, a metal-chelating assay, a superoxide radical scavenging assay, and a nitrite scavenging ability, as well as a DNA damage protection assay were performed. Total phenolic content was found to be 211.32 mg Tan/g WPD. The extract scavenged 50% DPPH free radical at a concentration of 21.35 ${\mu}g/mL$. At that same concentration, the reducing power ability of WPD was higher than that of ${\alpha}$-tocopherol. The extract chelated 68.9% ferrous ion at the concentration of 4 mg/mL. WPD showed better nitrite scavenging effect at the lower pH. Meanwhile, WPD exhibited a strong capability for DNA damage protection at 1 mg/mL concentration. Taken together, these data suggest water extract from Pinus densiflora could be used as a suitable natural antioxidant.

Keywords

References

  1. Ali SS, Kasoju N, Luthra A, Singh A, Sharanabasava H, Sahu A, Bora U. 2008. Indian medicinal herbs as sources of antioxidants. Food Res Int 41: 1-15. https://doi.org/10.1016/j.foodres.2007.10.001
  2. Hu W, Shen W, Wang MH. 2009. Free radical scavenging activity and protective ability of methanolic extract from Duchesnea indica against protein oxidation and DNA damage. J Food Sci Nutr 14: 277-282. https://doi.org/10.3746/jfn.2009.14.4.277
  3. Niki E. 2010. Assessment of antioxidant capacity in vitro and in vivo. Free Radic Biol Med 49: 503-515. https://doi.org/10.1016/j.freeradbiomed.2010.04.016
  4. Ratnam DV, Ankola DD, Bhardwaj V, Sahana DK, Kumar MNVR. 2006. Role of antioxidants in prophylaxis and therapy: a pharmaceutical perspective. J Control Release 113: 189-270. https://doi.org/10.1016/j.jconrel.2006.04.015
  5. Ende WVDE, Peshev D, Gara LD. 2011. Disease prevention by natural antioxidants and prebiotics acting as ROS scavengers in the gastrointestinal tract. Trends Food Sci Tech 22: 689-697. https://doi.org/10.1016/j.tifs.2011.07.005
  6. Mariod AA, Ibrahim RM, Ismail M, Ismail N. 2010. Antioxidant activities of phenolic rich fractions (PRFs) obtained from black mahlab (Monechma ciliatum) and white mahlab (Prunus mahaleb) seedcakes. Food Chem 118: 120-127. https://doi.org/10.1016/j.foodchem.2009.04.085
  7. Erkan N, Ayranci G, Ayranci E. 2008. Antioxidant activities of rosemary (Rosmarinus Officinalis L.) extract, blackseed (Nigella sativa L.) essential oil, carnosic acid, rosmarinic acid and sesamol. Food Chem 110: 76-82. https://doi.org/10.1016/j.foodchem.2008.01.058
  8. Kunyanga CN, Imungi JK, Okoth MW, Biesaiski HK, Vadivel V. 2012. Total phenolic content, antioxidant and antidiabetic properties of methanolic extract of raw and traditionally processed Kenyan indigenous food ingredients. LWT-Food Sci Technol 45: 269-276. https://doi.org/10.1016/j.lwt.2011.08.006
  9. Soong YY, Barlow PJ. 2004. Antioxidant activity and phenolic content of selected fruit seeds. Food Chem 88: 411-417. https://doi.org/10.1016/j.foodchem.2004.02.003
  10. Sun T, Ho CT. 2005. Antioxidant activities of buckwheat extracts. Food Chem 90: 743-749. https://doi.org/10.1016/j.foodchem.2004.04.035
  11. Lim JH, Kim JC, Kim KJ, Son YS, Sunwoo Y, Han JS. 2008. Seasonal variations of monoterpene emissions from Pinus densiflora in East Asia. Chemosphere 73: 470-478. https://doi.org/10.1016/j.chemosphere.2008.06.048
  12. Joo CG, Lee KH, Park C, Lee BC. 2011. Antioxidative activities and composition analysis of Pinus densiflora root by ultra high pressure extraction. J Ind Eng Chem 17: 712-716. https://doi.org/10.1016/j.jiec.2011.05.018
  13. Mun SP, Ku CS, Kim JP. 2010. Adsorption of metal and uranyl ions onto amidoximated Pinus densiflora bark. Wood Sci Technol 44: 283-299. https://doi.org/10.1007/s00226-009-0276-z
  14. EI-Zein O, Kreydiyyeh SI. 2011. Pine bark extract inhibits glucose transport in enterocytes via mitogen-activated kinase and phosphoinositol 3-kinase. Nutrition 27: 707-712. https://doi.org/10.1016/j.nut.2010.07.001
  15. Hu W, Heo SI, Wang MH. 2009. Antioxidant and anti-inflammatory activity of Kalopanax pictus leaf. J Korean Soc Appl Biol Chem 52: 360-366. https://doi.org/10.3839/jksabc.2009.064
  16. Singh N, Rajini PS. 2004. Free radical scavenging activity of an aqueous extract of potato peel. Food Chem 85: 611-616. https://doi.org/10.1016/j.foodchem.2003.07.003
  17. Bursal E, Gulcin I. 2011. Polyphenol contents and in vitro antioxidant activities of lyophilised aqueous extract of kiwifruit (Actinidia deliciosa). Food Res Int 44: 1482-1489. https://doi.org/10.1016/j.foodres.2011.03.031
  18. Li C, Son HJ, Huang C, Lee SK, Lohakare J, Wang MH. 2010. Comparison of Crataegus pinnatifida Bunge var. typica Schneider and C. pinnatifida Bunge fruits for antioxidant, anti-$\alpha$-glucosidase, and anti-inflammatory activities. Food Sci Biotechnol 19: 769-775. https://doi.org/10.1007/s10068-010-0108-9
  19. Apetrei CL, Tuchilus C, Aprotosoaie C, Opera A, Malterud KE, Miron A. 2011. Chemical, antioxidant and antimicrobial investigations of Pinus cembra L. bark and needles. Molecules 16: 7773-7788. https://doi.org/10.3390/molecules16097773
  20. Jung MJ, Chung HY, Choi JH, Choi JS. 2003. Antioxidant principles from the needles of red pine, Pinus densiflora. Phytother Res 17: 1064-1068. https://doi.org/10.1002/ptr.1302
  21. Ustun O, Senol FS, Kurkcuoglu M, Orhan IE, Kartal M, Baser KHC. 2012. Investigation on chemical composition, anticholinesterase and antioxidant activities of extracts and essential oil of Turkish Pinus species and pycnogenol. Ind Crop Prod 38: 115-123. https://doi.org/10.1016/j.indcrop.2012.01.016
  22. Lantto TA, Dorman HJD, Shikov AN, Pozharitskaya ON, Makarov VG, Tikhonov VP, Hitunen R, Raasmaja A. 2009. Chemical composition, antioxidative activity and cell viability effects of a Siberian pine (Pinus sibirica Du Tour) extract. Food Chem 112: 936-943. https://doi.org/10.1016/j.foodchem.2008.07.008
  23. Yu L, Zhao M, Wang J, Cui C, Yang B, Jiang Y, Zhao Q. 2008. Antioxidant, immunomodulatory and anti-breast cancer activities of phenolic extract from pine (Pinus massoniana Lamb) bark. Innov Food Sci Emerg 9: 122-128. https://doi.org/10.1016/j.ifset.2007.06.006
  24. Joo CG, Lee KH, Park C, Lee BC. 2011. Antioxidative activities and composition analysis of Pinus densiflora root by ultra high pressure extraction. J Ind Eng Chem 17: 712-716. https://doi.org/10.1016/j.jiec.2011.05.018
  25. Liu H, Wang L, Wang MH. 2011. Antioxidant and nitric oxide release inhibition activities of methanolic extract from Clerodendrum cyrtophyllum Turcz. Hortic Environ Biotechnol 52: 1-6. https://doi.org/10.1007/s13580-011-0058-z

Cited by

  1. Antioxidant Activity and Inhibitory Effect on Oxidative DNA Damage of Ethyl Acetate Fractions Extracted from Cone of Red Pine (Pinus densiflora) vol.29, pp.2, 2016, https://doi.org/10.7732/kjpr.2016.29.2.163
  2. Volatile Compounds and Antioxidant Capacity of the Bio-Oil Obtained by Pyrolysis of Japanese Red Pine (Pinus Densiflora Siebold and Zucc.) vol.20, pp.3, 2015, https://doi.org/10.3390/molecules20033986
  3. Brown Pine Leaf Extract and Its Active Component Trans-Communic Acid Inhibit UVB-Induced MMP-1 Expression by Targeting PI3K vol.10, pp.6, 2015, https://doi.org/10.1371/journal.pone.0128365
  4. Pinus densiflora Gnarl Inhibits Migration through Suppression of Protein Kinase C in C6 Glioma Cells vol.32, pp.2, 2015, https://doi.org/10.14406/acu.2015.006
  5. Evaluation of environment on polyphenols and flavonoids in oxalis corymbosa extracts as a potential source of antioxidants vol.170, pp.1755-1315, 2018, https://doi.org/10.1088/1755-1315/170/5/052034
  6. a high value tree species of Himalaya vol.152, pp.5, 2018, https://doi.org/10.1080/11263504.2017.1395372
  7. Antioxidative, Anti-inflammatory, and DNA Damage Protective Effect in Cortex Extracts of Eucommia ulmoides by Roasting vol.27, pp.4, 2012, https://doi.org/10.7783/kjmcs.2019.27.4.259
  8. The natural phytochemical trans‐communic acid inhibits cellular senescence and pigmentation through FoxO3a activation vol.28, pp.11, 2012, https://doi.org/10.1111/exd.14025
  9. An endophytic Penicillium oxalicum isolated from Citrus limon possesses antioxidant and genoprotective potential vol.128, pp.5, 2012, https://doi.org/10.1111/jam.14553
  10. Effects of Drought Stress on Anthocyanin Accumulation in Mulberry Fruits vol.20, pp.3, 2012, https://doi.org/10.3923/ajps.2021.450.460