DOI QR코드

DOI QR Code

Extract from Edible Red Seaweed (Gelidium amansii) Inhibits Lipid Accumulation and ROS Production during Differentiation in 3T3-L1 Cells

  • Seo, Min-Jung (Department of Biomedical Science, CHA University) ;
  • Lee, Ok-Hwan (Department of Food Science and Biotechnology, Kangwon National University) ;
  • Choi, Hyeon-Son (Department of Biomedical Science, CHA University) ;
  • Lee, Boo-Yong (Department of Biomedical Science, CHA University)
  • Received : 2012.05.01
  • Accepted : 2012.05.15
  • Published : 2012.06.30

Abstract

GPAR{elidium (G.) amansii is a red alga widely distributed in the shallow waters around East Asian countries. We investigated the effect of G. amansii on lipid accumulation and ROS (Reactive Oxygen Species) production in 3T3-L1 cells. G. amansii extracts dose-dependently inhibited lipid formation and ROS generation in cultured cells. Our results showed that anti-adipogenic effect of G. amansii was due to the reduction in mRNA expressions of PPAR${\gamma}$(peroxisome proliferator-activated receptor-${\gamma}$) and aP2 (adipocyte protein 2). G. amansii extracts significantly decreased mRNA levels of a ROS-generator, NOX4 (nicotinamide adenine dinucleotide phosphate hydrogen oxidase 4), and increased the protein levels of antioxidant enzymes including SOD1/2 (superoxide dismutases), Gpx (glutathione peroxidase), and GR (glutathione reductase), which can lead to the reduction of ROS in the cell. In addition, the G. amansii extract enhanced mRNA levels of adiponectin, one of the adipokines secreted from adipocytes, and GLUT4, glucose uptake protein. Taken together, our study shows that G. amansii extract inhibited lipid accumulation and ROS production by controlling adipogenic signals and ROS regulating genes.

Keywords

References

  1. Moller DE, Flier JS. 1991. Insulin resistance--mechanisms, syndromes, and implications. N Engl J Med 325: 938-948. https://doi.org/10.1056/NEJM199109263251307
  2. Scherer PE. 2006. Adipose tissue: from lipid storage compartment to endocrine organ. Diabetes 55: 1537-1545. https://doi.org/10.2337/db06-0263
  3. Adachi T, Toishi T. 2009. Expression of extracellular superoxide dismutase during adipose differentiation in 3T3-L1 cells. Redox Rep 14: 34-40. https://doi.org/10.1179/135100009X392467
  4. Furukawa S, Fujita T. 2004. Increased oxidative stress in obesity and its impact on metabolic syndrome. J Clin Invest 114: 1752-1761. https://doi.org/10.1172/JCI21625
  5. Vincent HK, Bourguignon CM, Vincent KR, Weltman AL, Bryant M, Taylor AG. 2006. Antioxidant supplementation lowers exercise-induced oxidative stress in young overweight adults. Obesity 14: 2224-2235. https://doi.org/10.1038/oby.2006.261
  6. Murphy MP. 2009. How mitochondria produce reactive oxygen species. J Biochem 417: 1-13. https://doi.org/10.1042/BJ20081386
  7. Attie AD. 2009. Adipocyte metabolism and obesity. J Lipid Res 50: S395-399. https://doi.org/10.1194/jlr.R800057-JLR200
  8. Rangwala SM, Lazar MA. 2000. Transcriptional control of adipogenesis. Annu Rev Nutr 20: 535-559. https://doi.org/10.1146/annurev.nutr.20.1.535
  9. Pearson SL, Cawthorne MA, Clapharm JC. 1996. The thiazolidinedione insulin sensitizer, BRL49653, increase the expression of PPARγ and aP2 in adipose tissue of high fat fed rats. Biochem Biophys Res Commun 229: 752-757. https://doi.org/10.1006/bbrc.1996.1876
  10. Guerre-Millo M. 2004. Adipose tissue and adipokines: for better or worse. Diabetes Metab 30: 13-19. https://doi.org/10.1016/S1262-3636(07)70084-8
  11. Monzillo LU, Hamdy O, Horton ES, Ledbury S, Mullooly C, Jarema C, Porter S, Ovalle K, Moussa A, Mantzoros CS. 2003. Effect of lifestyle modification on adipokine levels in obese subjects with insulin resistance. Obes Res 11: 1048-1054. https://doi.org/10.1038/oby.2003.144
  12. Yuan H, Song J. 2006. Immunomodulation and antitumor activity of kappa-carrageenan oligosaccharides. Cancer Lett 243: 228-234. https://doi.org/10.1016/j.canlet.2005.11.032
  13. Yan X, Nagata T. 1998. Antioxidative activities in some common seaweeds. Plant Foods Hum Nutr 52: 253-262. https://doi.org/10.1023/A:1008007014659
  14. Fu YW, Hou WY. 2007. The immunostimulatory effects of hot-water extract of Gelidium amansii via immersion, injection and dietary administrations on white shrimp Litopenaeus vannamei and its resistance against Vibrio alginolyticus. Fish Shellfish Immunol 22: 673-685. https://doi.org/10.1016/j.fsi.2006.08.014
  15. Lee OH, Seo MJ, Choi HS, Lee BY. 2011. Pycnogenol inhibits lipid accumulation in 3T3-L1 adipocytes with the modulation of reactive oxygen species (ROS) production associated with antioxidant enzyme responses. Phytother Res 26: 403-411.
  16. Peterson TR, Sengupta SS, Harris TE. 2011. mTOR complex 1 regulates lipin 1 localization to control the SREBP pathway. Cell 146: 408-420. https://doi.org/10.1016/j.cell.2011.06.034
  17. Rosen ED, Spiegelman BM. 2000. Molecular regulation of adipogenesis. Annu Rev Cell Dev Biol 16: 145-171. https://doi.org/10.1146/annurev.cellbio.16.1.145
  18. Houstis N, Rosen ED, Lander ES. 2006. Reactive oxygen species have a causal role in multiple forms of insulin resistance. Nature 440: 944-948. https://doi.org/10.1038/nature04634
  19. James DE, Brown R. 1998. Insulin-regulatable tissues express a unique insulin-sensitive glucose transport protein. Nature 333: 183-185.
  20. Lau DC, Dhillon B, Yan H, Szmitko PE, Verma S. 2005. Adipokines: molecular links between obesity and atheroslcerosis. Am J Physiol Heart Circ Physiol 288: H2031-H2041. https://doi.org/10.1152/ajpheart.01058.2004
  21. Fu Y, Luo N, Klein RL, Garvey WT. 2005. Adiponectin promotes adipocyte differentiation, insulin sensitivity, and lipid accumulation. J Lipid Res 46: 1369-1379. https://doi.org/10.1194/jlr.M400373-JLR200
  22. Chen YH, Tu CJ, Wu HT. 2004. Growth-inhibitory effects of the red alga Gelidium amansii on cultured cells. Biol Pharm Bull 27: 180-184. https://doi.org/10.1248/bpb.27.180
  23. Maeda H, Hosokawa M, Sashima T, Takahashi N, Kawada T, Miyashita K. 2006. Fucoxanthin and its metabolite, fucoxanthinol, suppress adipocyte differentiation in 3T3-L1 cells. Int J Mol Med 18: 147-152.
  24. Pantankar MS, Oehninger S, Barnett T, Williams RL, Clark GF. 1993. A revised structure for fucoidan may explain some of its biological activities. J Biol Chem 29: 21770-21776.
  25. Okai Y, Higashi-Okai K, Yano Y, Otani S. 1996. Identification of antimutagenic substances in an extract of edible red alga, Porphyra tenera (Asakusa-nori). Cancer Lett 100: 235-240. https://doi.org/10.1016/0304-3835(95)04101-X

Cited by

  1. Red algae ( Gelidium amansii ) hot-water extract ameliorates lipid metabolism in hamsters fed a high-fat diet 2017, https://doi.org/10.1016/j.jfda.2016.12.008
  2. Popular edible seaweed, Gelidium amansii prevents against diet-induced obesity vol.90, 2016, https://doi.org/10.1016/j.fct.2016.02.014
  3. Red seaweeds for obesity prevention? vol.94, 2016, https://doi.org/10.1016/j.fct.2016.05.022
  4. Gelidium elegans Regulates the AMPK-PRDM16-UCP-1 Pathway and Has a Synergistic Effect with Orlistat on Obesity-Associated Features in Mice Fed a High-Fat Diet vol.9, pp.4, 2017, https://doi.org/10.3390/nu9040342
  5. Silymarin inhibits adipogenesis in the adipocytes in grass carp Ctenopharyngodon idellus in vitro and in vivo 2017, https://doi.org/10.1007/s10695-017-0387-7
  6. Gelidium amansiiextract ameliorates obesity by down-regulating adipogenic transcription factors in diet-induced obese mice vol.11, pp.1, 2017, https://doi.org/10.4162/nrp.2017.11.1.17
  7. Gelidium amansii ethanol extract suppresses fat accumulation by down-regulating adipogenic transcription factors in ob/ob mice model vol.26, pp.1, 2017, https://doi.org/10.1007/s10068-017-0028-z
  8. vol.2018, pp.1942-0994, 2018, https://doi.org/10.1155/2018/9354296
  9. Flavonoids, Potential Bioactive Compounds, and Non-Shivering Thermogenesis vol.10, pp.9, 2018, https://doi.org/10.3390/nu10091168
  10. Nutrients and bioactive potentials of edible green and red seaweed in Korea vol.21, pp.7, 2012, https://doi.org/10.1186/s41240-018-0095-y
  11. Anti-inflammatory effects of Agar free-Gelidium amansii (GA) extracts in high-fat diet-induced obese mice vol.12, pp.6, 2012, https://doi.org/10.4162/nrp.2018.12.6.479
  12. The Anti-Obesity Effect of Polysaccharide-Rich Red Algae (Gelidium amansii) Hot-Water Extracts in High-Fat Diet-Induced Obese Hamsters vol.17, pp.9, 2012, https://doi.org/10.3390/md17090532
  13. Anti-Obesity Effects of Grateloupia elliptica , a Red Seaweed, in Mice with High-Fat Diet-Induced Obesity via Suppression of Adipogenic Factors in White Adipose Tissue and Increased Thermogenic Facto vol.12, pp.2, 2012, https://doi.org/10.3390/nu12020308
  14. Therapeutic Uses of Red Macroalgae vol.25, pp.19, 2012, https://doi.org/10.3390/molecules25194411
  15. Cellulose nanocrystal preparation from Gelidium amansii and analysis of its anti-inflammatory effect on the skin in vitro and in vivo vol.254, pp.None, 2012, https://doi.org/10.1016/j.carbpol.2020.117315
  16. Sulfated polysaccharides from red seaweed Gelidium amansii: Structural characteristics, anti-oxidant and anti-glycation properties, and development of bioactive films vol.119, pp.None, 2012, https://doi.org/10.1016/j.foodhyd.2021.106820