Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Anti-apoptotic Activity of Ginsenoside Rb1 in Hydrogen Peroxide-treated Chondrocytes: Stabilization of Mitochondria and the Inhibition of Caspase-3

  • Na, Ji-Young (BioSafety Research Institute, College of Veterinary Medicine, Chonbuk National University) ;
  • Kim, Sok-Ho (BioSafety Research Institute, College of Veterinary Medicine, Chonbuk National University) ;
  • Song, Ki-Bbeum (BioSafety Research Institute, College of Veterinary Medicine, Chonbuk National University) ;
  • Lim, Kyu-Hee (BioSafety Research Institute, College of Veterinary Medicine, Chonbuk National University) ;
  • Shin, Gee-Wook (BioSafety Research Institute, College of Veterinary Medicine, Chonbuk National University) ;
  • Kim, Jong-Hoon (BioSafety Research Institute, College of Veterinary Medicine, Chonbuk National University) ;
  • Kim, Bum-Seok (BioSafety Research Institute, College of Veterinary Medicine, Chonbuk National University) ;
  • Kwon, Young-Bae (Department of Pharmacology, School of Medicine, Chonbuk National University) ;
  • Kwon, Jung-Kee (BioSafety Research Institute, College of Veterinary Medicine, Chonbuk National University)
  • Received : 2011.11.09
  • Accepted : 2012.02.10
  • Published : 2012.07.15
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Abstract

Chondrocyte apoptosis has been recognized as an important factor in the pathogenesis of osteoarthritis (OA). Hydrogen peroxide ($H_2O_2$), which produces reactive oxygen species, reportedly induces apoptosis in chondrocytes. The ginsenoside $Rb_1$ (G-$Rb_1$) is the principal component in ginseng and has been shown to have a variety of biological activities, such as anti-arthritis, anti-inflammation, and anti-tumor activities. In this study, we evaluated the effects of G-$Rb_1$ on the mitochondrial permeability transition (MPT) and caspase-3 activity of chondrocyte apoptosis induced by $H_2O_2$. Cultured rat articular chondrocytes were exposed to $H_2O_2$ with or without G-$Rb_1$ and assessed for viability, MPT, Bcl-xL/Bax expression, caspase-3 activity, and apoptosis. The co-treatment with G-$Rb_1$ showed an inhibition of MPT, caspase-3 activity, and cell death. Additionally, the levels of the apoptotic protein Bax were significantly lower and the levels of the anti-apoptotic protein Bcl-xL were higher compared with $H_2O_2$ treatment alone. The results of this study demonstrate that G-$Rb_1$ protects chondrocytes against $H_2O_2$-induced apoptosis, at least in part via the inhibition of MPT and caspase-3 activity. These results demonstrate that G-$Rb_1$ is a potentially useful drug for the treatment of OA patients.

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References

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