Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Anti-aging Effects of Solvent Fraction from Agrimonia pilosa L. Extracts

선학초 용매 분획물의 항노화 효과

  • Yoon, Ji-Young (Department of Herbal Cosmetic Science, Hoseo University) ;
  • Lee, Soo-Yeon (Department of Herbal Cosmetic Science, Hoseo University) ;
  • Jun, Hye-Ji (Department of Herbal Cosmetic Science, Hoseo University) ;
  • Lee, Jin-Young (Department of Herbal Cosmetic Science, Hoseo University)
  • Received : 2011.10.13
  • Accepted : 2011.12.06
  • Published : 2012.03.31
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Abstract

The purpose of this study was to research about the ingredients for anti-oxidation and anti-wrinkle effects of the solvent fractions from Agrimonia pilosa L. hot water (AW) and 70% ethanol (AE) extracts. The electron donating ability of the solvent fractions from AW and AE extracts showed 84.9, 92.5% in ethyl acetate layer of AW and AE at 1,000 ppm. The superoxide dismutase like activity of solvent fractions from AW and AE extracts showed 61.8% in ethyl acetate layer of AW extracts and 58.0% in buthanol layer of AE extracts at 1,000 ppm. For anti-wrinkle effect, elastase inhibition effect of the solvent fractions from AW and AE extracts showed 55.2, 70.1% in ethyl acetate layer of AW and AE extracts at 1,000 ppm. And collagenase inhibition effect of the solvent fractions from AW and AE extracts showed highest inhibition effect as 90.6% in ethyl acetate layer of AW extract and 88.6% in $n$-butyl alcohol layer of AE extract at 1,000 ppm. All these findings suggested that solvent fractions from AW and AE extracts has an anti-oxidation and anti-wrinkle effects.

선학초 열수 추출물과 에탄올 추출물의 분획물을 항산화 및 주름개선의 소재로 이용하기 위하여 생리활성을 검증하였다. 항산화효과를 확인하기 위하여 전자공여능을 측정한 결과 선학초 열수추출물(AW)과 에탄올추출물(AE) 모두 ethyl acetate층 1,000 ppm에서 각각 96.2, 92.3%의 효과를 나타내었고, SOD 유사활성능을 측정한 결과 AW의 ethyl acetate층에서 61.8%의 활성을 AE의 $n$-butyl alcohol 층에서 58.0%의 높은 활성을 각각 나타내었다. 또한 xanthine oxidase저해활성을 측정한 결과 AW와 AE에서 모두 ethyl acetate층 1,000 ppm일 때, 각각 84.9, 92.5%의 가장 높은 저해율을 나타내었다. 주름개선 효능 확인을 위해 elastase 저해활성을 측정한 결과 이 또한 AW와 AE 모두에서 ethyl acetate층이 각각 55.2, 70.1%의 효능을 나타내었으며, collagenase 저해활성을 측정한 결과 AW의 ethyl acetate층이 90.6%로 가장 높은 저해율을, AE는 88.6%로 $n$-butyl alcohol 층이 가장 높은 저해율을 나타내었다. 이상의 결과로 미루어 보아 선학초의 열수 추출물과 에탄올 추출물의 분획물이 in vitro상에서 항산화 및 주름개선의 활성이 있음을 확인할 수 있었다.

Keywords

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