Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
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Dietary effect of red ginseng extracts mixed with torilis fructus and corni fructus on the epidermal levels of ceramides and ceramide related enzyme proteins in uv-induced hairless mice

자외선이 조사된 무모생쥐에서 홍삼, 사상자, 산수유 혼합 추출물 섭취가 표피 세라마이드 함량 및 관련 효소의 발현에 미치는 영향

  • Lee, Yun-Ju (Department of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Oh, Inn-Gyung (Department of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Cho, Yun-Hi (Department of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University)
  • 이윤주 (경희대학교 동서의학대학원 의학영양학과) ;
  • 오인경 (경희대학교 동서의학대학원 의학영양학과) ;
  • 조윤희 (경희대학교 동서의학대학원 의학영양학과)
  • Received : 2011.12.14
  • Accepted : 2012.04.03
  • Published : 2012.06.30
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Abstract

UV-irradiation is a major factor of photo-aged skin, by which pigmentation, wrinkles and laxity are increased. In addition, the epidermal barrier is disrupted, ultimately causing dryness in photo-aged skin. As an effort to search dietary sources for improving the dryness of UV irradiated skin, the dietary effect of red ginseng based functional foods on the epidermal level of ceramides, a major lipid maintaining epidermal barrier, was determined in this study. Albino hairless mice were fed either a control diet [group UV (UV-irradiated control)] or diets with 0.5% (group M0.5) or 1% (group M1.0) of red ginseng extracts mixed with Torilis fructus and Corni fructus (66.7% red ginseng) in parallel with UV irradiation for 5 wks. A normal control group (group C) was fed a control diet without UV irradiation for 5 wks. The epidermal level of ceramides in group UV was significantly lower than that in group C, in which ceramidase, an enzyme involved in ceramide degradation, was highly expressed. In group M0.5, the epidermal level of ceramide was significantly increased to the level even higher than in group C. In addition, protein expression of serine palmitoyl transferase (SPT), a key enzyme involved in de novo ceramide synthesis, was increased in group M0.5. However the epidermal levels of ceramides as well as of ceramidase protein expression in group M1.0 did not differ from those in group UV. In conclusion, we demonstrate that dietary supplementation of red-ginseng extracts mixed with Torilis fructus and Corni fructus at a level of 0.5% level in diet increased the epidermal level of ceramides coupled with the elevated expression of SPT protein.

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References

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