Korean Journal of Acupuncture

Society for Meridian and Acupoint (경락경혈학회)
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Induction of Apoptosis in AGS Human Gastric Cancer Cell by Ethanol Extract of Ganoderma lucidum

영지 약침액이 인체 위암 세포 성장억제 및 세포사멸 유발에 미치는 영향

  • Lee, Byung-Hoon (Myung Oriental Medicine Clinic in Busan) ;
  • Kim, Hong-Gi (Department of Oriental Medicine, Graduate School, Dongeui University) ;
  • Kim, Cheol-Hong (Department of Oriental Medicine, Graduate School, Dongeui University) ;
  • Youn, Hyoun-Min (Department of Oriental Medicine, Graduate School, Dongeui University) ;
  • Song, Choon-Ho (Department of Oriental Medicine, Graduate School, Dongeui University) ;
  • Jang, Kyung-Jeon (Department of Oriental Medicine, Graduate School, Dongeui University)
  • 이병훈 (부산 명한의원) ;
  • 김홍기 (동의대학교 한의과대학 침구학교실) ;
  • 김철홍 (동의대학교 한의과대학 침구학교실) ;
  • 윤현민 (동의대학교 한의과대학 침구학교실) ;
  • 송춘호 (동의대학교 한의과대학 침구학교실) ;
  • 장경전 (동의대학교 한의과대학 침구학교실)
  • Received : 2012.05.17
  • Accepted : 2012.06.04
  • Published : 2012.06.27
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Abstract

Objectives : Ganoderma lucidum(Ganoderma or lingzhi, 靈芝) is a well-known oriental medical mushroom containing many bioactive compounds. The possible mechanisms involved in its effects on cancer cells remain to be elucidated. In the present study, the anti-proliferative and apoptotic activities of the G. lucidum ethanol extract(GEE), in AGS human gastric cancer cells were investigated. Methods : It was found that exposure of AGS cells to GEE resulted in the growth inhibition in a dose and time dependent manner as measured by trypan blue count and MTT assay. The anti-proliferative effect of GEE treatment in AGS cells was associated with morphological changes and formation of apoptotic bodies, and the flow cytometry analysis confirmed that GEE treatment increased the populations of apoptotic-sub G1 phase. Growth inhibition and apoptosis of AGS cells by GEE were connected with a concentration and time-dependent up-regulation of tumour necrosis factor-related apoptosis-inducing ligand(TRAIL) expression. Results : The levels of XIAP and survivin expression, members of IAP family proteins, were gradually down-regulated by GEE treatment. However other members of IAP family proteins such as cIAP-1 and cIAP-2 remained unchanged in GEE-treated AGS cells. GEE treatment also induced the proteolytic activation of caspase-3, caspase-8 and caspase-9 and a concomitant degradation of poly(ADP-ribose) polymerase(PARP) protein, a caspase-3 substrate protein. Additionally, GEE-induced apoptosis was associated with the inhibition of Akt activation in a concentration and time-dependent manner, and pre-treatment with LY294002, a phosphoinositide 3-kinase(PI3K)/Akt inhibitor, significantly increased GEE-induced growth inhibition and apoptosis. Conclusions : Therefore, G. lucidum has a strong potential as a therapeutic agent for preventing cancers such as gastric cancer cells.

Keywords

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