Korean Journal of Acupuncture

Society for Meridian and Acupoint (경락경혈학회)
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Effects of Acupuncture at the Sea Point on the Changes of Plasma and Tissue Levels of NO, nNOS, Norepinephrine in Rats

12경맥(經脈)의 오수혈중(五輸穴中) 합혈(合穴) 침자(鍼刺)가 백서(白鼠)의 혈위(穴位) 조직내 NO, nNOS와 조직 및 혈장 Norepinephrine의 변화에 미치는 영향(影響)

  • Kim, Young-Sun (College of Oriental Medicine, Dongshin University) ;
  • Choi, Dong-Hee (College of Oriental Medicine, Dongshin University) ;
  • Choi, Tae-Jin (College of Oriental Medicine, Dongshin University) ;
  • Jang, Ho-Sun (College of Oriental Medicine, Dongshin University) ;
  • Na, Chang-Su (College of Oriental Medicine, Dongshin University) ;
  • Shin, Heon-Tae (College of Oriental Medicine, Dongshin University) ;
  • Lee, Kyoung-In (Biotechnology Industrialization Center, Dongshin University) ;
  • Kim, Sun-Min (Biotechnology Industrialization Center, Dongshin University) ;
  • Pyo, Byoung-Sik (Biotechnology Industrialization Center, Dongshin University) ;
  • Youn, Dae-Hwan (College of Oriental Medicine, Dongshin University)
  • 김영선 (동신대학교 한의과대학) ;
  • 최동희 (동신대학교 한의과대학) ;
  • 최태진 (동신대학교 한의과대학) ;
  • 장호선 (동신대학교 한의과대학) ;
  • 나창수 (동신대학교 한의과대학) ;
  • 신헌태 (동신대학교 한의과대학) ;
  • 이경인 (동신대학교 생물자원산업화지원센터) ;
  • 김선민 (동신대학교 생물자원산업화지원센터) ;
  • 표병식 (동신대학교 생물자원산업화지원센터) ;
  • 윤대환 (동신대학교 한의과대학)
  • Received : 2012.05.22
  • Accepted : 2012.06.08
  • Published : 2012.06.27
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Abstract

Objectives : This study is to observe the changes in the expression of neurotransmitters, such as NO, nNOS, and NE, upon the needle insertion to the sea points, which is one of the five transport points. Methods : Needles were inserted into rats, on both left and right sides of all sea points, including the LU5, PC3, HT3, LI11, TE10, SI8, SP9, LR8, KI10, ST36, GB34, and BL40, which are the sea points of five transport points for 12 meridian vessels. After insertion, needles were retained for five minutes. After the retention, blood was drawn via cardiac puncture, and tissues of each point near meridian vessels were extracted to examine the changes in the expression of NO, nNOS and NE. Results : In terms of the effect in NO production, there was a significant decrease only in the LU5 point, whereas there was a significant increase in the TE10 point alone. In terms of the expression of nNOS within tissues, none of the experimental groups showed significant changes based on the results of immunohistochemistry and western blotting. Regarding the formation of norepinephrine within tissues, the HT3, SP9, and KI10 point showed a significant decrease, while the PC3 and LR8 point showed a significant increase. Production of plasma norepinephrine was significantly increased at the TE10, SP9, LR8, GB34, and BL40 point. Conclusions : The effect of needles applied at the sea points of five transport points of 12 meridian vessels on the functions of NO, nNOS, and NE could be observed, and it is considered that the effect of needle stimulation on nervous system disorders could be studied through additional researches based on this one.

Keywords

References

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