Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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A Comparative Study on the Physiological Activities of Auricularia spp.

목이버섯 품종간 생리활성 비교 연구

  • Jo, Se-Hyun (Department of Food Science and Biotechnology, Sungkyunkwan University) ;
  • Kim, Tae-Ho (Department of Food Science and Biotechnology, Sungkyunkwan University) ;
  • Yu, Young-Bok (Mushroom research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Oh, Jin-A (Mushroom research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Jang, Mi-Hyang (Jeonnam Agricultural Research & Extension Services) ;
  • Park, Ki-Moon (Department of Food Science and Biotechnology, Sungkyunkwan University)
  • 조세현 (성균관대학교 식품생명공학과) ;
  • 김태호 (성균관대학교 식품생명공학과) ;
  • 유영복 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 오진아 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 장미향 (전남농업기술원) ;
  • 박기문 (성균관대학교 식품생명공학과)
  • Received : 2012.02.08
  • Accepted : 2012.03.20
  • Published : 2012.06.30
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Abstract

This study investigated the physiological effects of three species of Auricularia known as Auricularia polytricha (JNM21001), Auricularia auricula-judae black (JNM21002), and Auricularia auricula-judae brown (JNM21012). In the ORAC assay, Auricularia spp. showed antioxidant activities in the order of JNM21001>JNM21012>JNM21002. All Auricularia spp. strongly inhibited the action of ${\alpha}$-amyloglucosidase up to 60%. In order to further test in vivo anti-obesity effects, high fat diet induced ICR obese mice fed a diet containing 20% fat were used. All Auricularia spp. supplementation during high fat diet feeding significantly reduced body weight gain, epididymal fat pads weight, and lowered the food efficiency ratio compared to the high fat control (HFC) group. In particular, the group fed with JNM21012 had a lower average daily body weight gain of 0.45 g/day, demonstrating similar levels to the normal diet fed group. The group fed with JNM21012 significantly reduced lowered serum triglycerides (42%), total cholesterol (81%), and LDL-cholesterol level (66%) compared with the HFC group.

본 연구에서는 목이버섯 품종간의 생리활성을 실험하였다. ORAC assay로 항산화활성을 측정한 결과 털목이, 갈색목이, 흑목이 순으로 활성이 높게 나타났다. ${\alpha}$-Amyloglucosidase 저해활성을 통한 항당뇨 활성은 3품종 모두 10 mg/mL에서 60% 정도의 높은 저해활성을 나타냈으며 품종간 유의적인 차이는 나타나지 않았다. Raw 264.7 macrophage의 nitric oxide 생성 저해를 통한 항염활성은 1 mg/mL 처리시 털목이와 흑목이는 dexamethasone 1 ${\mu}g$/mL에 상응하는 활성을 나타냈다. 20% 지방을 첨가하여 고지방식이로 비만을 유도한 ICR 마우스의 목이버섯 급여에 따른 항비만 효과를 확인한 결과 모든 군에서 체중 및 증체량, 식이효율의 감소를 확인할 수 있었고 그 중 갈색목이버섯인 JNM21012군은 0.45 g/day로 7% 지방을 첨가한 일반식이(NC) 군과 동일 수준의 증체량을 보였다. 부고환 무게는 털목이 및 흑목이, 갈색목이 모두 고지방식이(HFC) 군에 비해서 낮은 수치를 보였으며, 갈색목이 군은 HFC군과 유의적인 차이를 보였다. 혈액성분 중 triglyceride의 경우 목이버섯 첨가 식이군 모두 HFC군에 비해 낮은 수치를 보였으며 갈색목이 군은 고지방식이 HFC군에 비해 42% 감소시키는 것으로 나타났다. 또한, total cholesterol과 LDL-cholesterol은 털목이와 갈색목이군이 HFC군 보다 낮은 수치를 나타냈으며, 갈색목이군은 HFC군에 비해 각각 81%와 66% 감소하여 유의적인 차이를 보였다. HDL-cholestrol의 경우 각 군간의 유의적인 차이를 보이지 않았지만 NC군에 비해 높은 수치를 나타냈다.

Keywords

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