Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Antioxidant Effects of Extracts from Fermented Red Ginseng Added with Medicinal Herbs in STZ-induced Diabetic Rats

약용식물 첨가 발효홍삼 추출물의 Streptozotocin 유발 당뇨쥐에 대한 항산화 효과

  • Kim, Hyun-Jeong (The Center for Traditional Microorganism Resources, Keimyung University) ;
  • Lee, Sung-Gyu (Department of Food and Technology, Keimyung University) ;
  • Park, Sung-Jin (Department of Food and Technology, Keimyung University) ;
  • Yu, Mi-Hee (Department of Food and Technology, Keimyung University) ;
  • Lee, Eun-Ju (Bio Research Institute, NUC Electronics Co. Ltd.) ;
  • Lee, Sam-Pin (The Center for Traditional Microorganism Resources, Keimyung University) ;
  • Lee, In-Seon (Department of Food and Technology, Keimyung University)
  • 김현정 (계명대학교 전통미생물자원개발 및 연구산업화 센터) ;
  • 이성규 (계명대학교 식품가공학과) ;
  • 박성진 (계명대학교 식품가공학과) ;
  • 유미희 (계명대학교 식품가공학과) ;
  • 이은주 ((주)NUC전자 바이오연구소) ;
  • 이삼빈 (계명대학교 전통미생물자원개발 및 연구산업화 센터) ;
  • 이인선 (계명대학교 식품가공학과)
  • Received : 2012.02.24
  • Accepted : 2012.03.30
  • Published : 2012.06.30
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Abstract

Antioxidant and anti-hyperglycemic activities of fermented red ginseng added with 5 kinds of medicinal herbs (FRGM) were investigated in vitro. Total polyphenol and total flavonoid contents in FRGM extracts were $22.41{\pm}3.51$ and $16.80{\pm}4.22{\mu}g/mg$, respectively. FRGM extracts were capable of directly scavenging DPPH free radicals ($RC_{50}=95.57{\pm}7.40{\mu}g/mL$), and then showed higher inhibitory activities for ${\alpha}$-glucosidase. This study was also conducted to evaluate the effects of FRGM extracts in streptozotocin (STZ)-induced diabetic (DM) rats. The activities with regards to serum aspartate aminotransferase and alanine aminotransferase were significantly decreased by FRGM extracts compared to those from the STZ group. The hepatic glutathione content depleted by STZ was significantly increased by FRGM extracts, but elevation of lipid peroxide content induced by STZ was significantly decreased by FRGM extracts. The decreased activities of superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase after STZ-treatment were increased through the treatment of FRGM extracts. These results indicated that fermented red ginseng added with medicinal herbs can protect against STZ-induced diabetic rats through its antioxidant properties.

5종의 약용식물을 첨가한 발효홍삼(FRGM) 추출물의 항산화활성 및 ${\alpha}$-glucosidase 저해 활성을 in vitro에서 검토하였고, 또한 STZ로 당뇨를 유발시킨 흰쥐에서 FRGM 추출물의 효능을 살펴보았다. 그 결과, FRGM 추출물은 총 폴리페놀 및 총 플라보노이드 함량이 각각 $22.41{\pm}3.51{\mu}g/mg$, $16.80{\pm}4.22{\mu}g/mg$으로 존재하였고, DPPH 소거 활성을 가지고($RC_{50}=95.57{\pm}7.40{\mu}g/mL$), 또한 높은 ${\alpha}$-glucosidase 저해 활성을 나타내었다. STZ 투여에 의해 증가된 혈청 내 ALT와 AST의 활성이 FRGM 추출물의 투여로 감소하였으며, 간 조직중의 글루타치온 함량은 STZ 투여군에서는 감소되었다가 FRGM 투여시 유의적인 증가를 보였고, 지질과산화물 함량은 STZ 투여시 증가되었다가 FRGM 추출물의 투여시 유의적으로 감소되었다. 또한 간 조직중의 SOD, CAT, GP 그리고 GR의 활성도 STZ 투여로 유의적으로 감소되었다가, FRGM 추출물의 투여로 이들 항산화효소 활성이 유의적으로 증가하였다.

Keywords

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