Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effect of Anisi Stellati Fructus Water Extract on Hydrogen Peroxide Production in RAW 264.7 Mouse Macrophages

대회향(大茴香) 물추출물이 마우스 대식세포주(RAW 264.7 cell line)의 hydrogen peroxide 생성에 미치는 영향

  • Lee, Ji-Young (Department of Pathology, College of Oriental Medicine, Gachon University) ;
  • Kim, Young-Jin (Department of Pathology, College of Oriental Medicine, Gachon University) ;
  • Kim, Hyung-Joo (Department of Pathology, College of Oriental Medicine, Gachon University) ;
  • Lee, Min-Woo (Department of Genetic Engineering, College of Life Science, Kyung Hee University) ;
  • Park, Wan-Su (Department of Pathology, College of Oriental Medicine, Gachon University)
  • 이지영 (가천대학교 한의과대학 병리학교실) ;
  • 김영진 (가천대학교 한의과대학 병리학교실) ;
  • 김현주 (가천대학교 한의과대학 병리학교실) ;
  • 이민우 (경희대학교 생명과학대학 유전공학과) ;
  • 박완수 (가천대학교 한의과대학 병리학교실)
  • Received : 2012.02.25
  • Accepted : 2012.05.31
  • Published : 2012.06.25
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Abstract

The purpose of this study is to investigate effects of Anisi stellati Fructus Water Extract on hydrogen peroxide production in RAW 264.7 mouse macrophages. Anisi stellati Fructus were extracted by hot water. Effects of Anisi stellati Fructus water extract (AS) on hydrogen peroxide production in RAW 264.7 were measured by dihydrorhodamine 123 assay after 20, 24, 28, 44, 48, and 52 h incubation at the concentrations of 10, 25, 50, and $100{\mu}g/mL$. For 20 h incubation, AS significantly increased hydrogen peroxide production in RAW 264.7 cells by $108.6{\pm}1.56%$, $109.5{\pm}1.94%$, $108.4{\pm}1.14%$, and $107.3{\pm}3.06%$ at the concentrations of 10, 25, 50, and $100{\mu}g/mL$ (P < 0.05) respectively. For 24, 28, 44, 48, and 52 h incubation, AS also significantly increased hydrogen peroxide production in RAW 264.7 cells at the concentrations of 10, 25, 50, and $100{\mu}g/mL$ (P < 0.05). These results suggest that Anisi stellati Fructus has the immune - enhancing property related with its increase of hydrogen peroxide production in macrophages.

Keywords

References

  1. 박성호, 성인화. 소회향, 대회향 추출물과 정유의 Candida albicans에 대한 항진균 효과. 대한의진균학회지 15(4):157-164, 2010.
  2. Chung, H.J. Evaluation of the Biological Activity of Extracts from Star-Anise (Illicium verum). Journal of food science and nutrition. 14(3):195-200, 2009. https://doi.org/10.3746/jfn.2009.14.3.195
  3. 조형찬. 닭진드기 성충에 대한 대회향 유래 아네톨 및 유사 화합물들의 살비활성. 한국응용곤충학회지 48(2):263-268, 2009.
  4. 류희영, 안선미, 신용규, 손호용. 한약재의 항균 활성 및 인간 적혈구 용혈 활성. 한국미생물.생명공학회지 38(2):190-197, 2010.
  5. 박완수. Lipopolysaccharide로 활성화된 마우스 대식세포에서 艾葉물추출물의 면역활성 연구. 대한본초학회지 24(1):151-157, 2009.
  6. 이지영, 이영종, 박완수. 발효 어성초(魚腥草) 물추출물의 마 우스 대식세포 항염활성 연구. 대한본초학회지 25(3):27-34, 2010.
  7. Newman, S.L. Macrophages in host defense against Histoplasma capsulatum. Trends Microbiol. 7(2):67-71, 1999. https://doi.org/10.1016/S0966-842X(98)01431-0
  8. Supek, F., Supekova, L., Nelson, H., Nelson, N. Function of metal-ion homeostasis in the cell division cycle, mitochondrial protein processing, sensitivity to mycobacterial infection and brain function. J Exp Biol. 200(Pt 2):321-330, 1997.
  9. Naito, M. Macrophage differentiation and function in health and disease. Pathol Int. 58(3):143-155, 2008. https://doi.org/10.1111/j.1440-1827.2007.02203.x
  10. Lingnau, M., Hflich, C., Volk, H.D., Sabat, R., Dcke, W.D. Interleukin-10 enhances the CD14-dependent phagocytosis of bacteria and apoptotic cells by human monocytes. Hum Immunol. 68(9):730-738, 2007. https://doi.org/10.1016/j.humimm.2007.06.004
  11. Guha, M., Mackman, N. LPS induction of gene expression in human monocytes. Cell Signal. 13(2):85-94, 2001. https://doi.org/10.1016/S0898-6568(00)00149-2
  12. 박완수. 黃芩물추출물이 마우스 대식세포의 hydrogen peroxide 생성에 미치는 영향. 대한본초학회지 26(1):53-58, 2011.
  13. 박완수. 마우스 대식세포(Raw 264.7)에 대한 한약조성물 KOCO-P1의 세포활성 연구. 대한본초학회지 23(2):151-157, 2008.
  14. 박완수. 인간 간조직세포(HepG2 Cells)에 대한 한약조성물 KOCO-P1의 효과 연구. 대한본초학회지23(3):149-154, 2008.
  15. Yoon, S.B., Lee, Y.J., Park, S.K., Kim, H.C., Bae, H., Kim, H.M., Ko, S.G., Choi, H.Y., Oh, M.S., Park, W. Anti-inflammatory effects of Scutellaria baicalensis water extract on LPS-activated RAW 264.7 macrophages. J Ethnopharmacol. 125(2):286-290, 2009. https://doi.org/10.1016/j.jep.2009.06.027
  16. Richardson, M.P., Ayliffe, M.J., Helbert, M., Davies, E.G. A simple flow cytometry assay using dihydrorhodamine for the measurement of the neutrophil respiratory burst in whole blood: comparison with the quantitative nitrobluetetrazolium test. J Immunol Methods. 219(1-2):187-193, 1998. https://doi.org/10.1016/S0022-1759(98)00136-7
  17. Crow, J.P. Dichlorodihydrofluorescein and dihydrorhodamine 123 are sensitive indicators of peroxynitrite in vitro: implications for intracellular measurement of reactive nitrogen and oxygen species. Nitric Oxide. 1(2):145-157, 1997. https://doi.org/10.1006/niox.1996.0113
  18. Jirapongsananuruk, O., Malech, H.L., Kuhns, D.B., Niemela, J.E., Brown, M.R., Anderson-Cohen, M., Fleisher, T.A. Diagnostic paradigm for evaluation of male patients with chronic granulomatous disease, based on the dihydrorhodamine 123 assay. J Allergy Clin Immunol. 111(2):374-379, 2003. https://doi.org/10.1067/mai.2003.58
  19. 박완수. 白蔘과 紅蔘이 포함된 理中湯의 마우스 대식세포 내 hydrogen peroxide 생성에 미치는 영향. 동의생리병리학회지 25(1):78-83, 2011.
  20. 한효상, 박완수, 이영종. 애엽(艾葉) 발효 추출물의 면역활성에 관한 연구. 대한본초학회지 23(3):103-112, 2008.