대한약침학회지 (Journal of Pharmacopuncture)

  • 제15권2호
  • /
  • Pages.20-23
  • /
  • 2012
  • /
  • 2093-6966(pISSN)
  • /
  • 2234-6856(eISSN)
대한약침학회 (KOREAN PHARMACOPUNCTURE INSTITUTE)
권호 표지
DOI QR코드

DOI QR Code

Identification and Analysis of the Chloroplast rpoC1 Gene Differentially Expressed in Wild Ginseng

  • Lee, Kwang-Ho (Department of Acupuncture & Moxibustion, Sangji University College of Oriental Medicine) ;
  • Kwon, Ki-Rok (Research Center of the Korean Pharmacopuncture Institute) ;
  • Kang, Won-Mo (Department of Biochemistry, Graduate School of Medicine, Inha University) ;
  • Jeon, Eun-Mi (Department of Biochemistry, Graduate School of Medicine, Inha University) ;
  • Jang, Jun-Hyeog (Department of Biochemistry, Graduate School of Medicine, Inha University)
  • 투고 : 2012.04.04
  • 심사 : 2012.06.20
  • 발행 : 2012.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Panax ginseng is a well-known herbal medicine in traditional Asian medicine, and wild ginseng is widely accepted to be more active than cultivated ginseng in chemoprevention. However, little has actually been reported on the difference between wild ginseng and cultivated ginseng. Thus, to identify and analyze those differences, we used suppressive subtraction hybridization (SSH) sequences with microarrays, realtime polymerase chain reaction (PCR), and reverse transcription PCRs (RT-PCRs). One of the clones isolated in this research was the chloroplast rpoC1 gene, a ${\beta}$subunit of RNA polymerase. Real-time RT-PCR results showed that the expression of the rpoC1 gene was significantly upregulated in wild ginseng as compared to cultivated ginseng, so, we conclude that the rpoC1 gene may be one of the important markers of wild ginseng.

키워드

참고문헌

  1. Zhang D, Yasuda T, Yu Y, Zheng P, Kawabata T, Ma Y, et al. Ginseng extract scavenges hydroxyl radical and protects unsaturated fatty acids from decomposition caused by ironmediated lipid peroxidation. Free Radic Biol Med. 1996;20(1):145-50. https://doi.org/10.1016/0891-5849(95)02020-9
  2. Yun TK, Lee YS, Lee YH, Kim SI, Yun HY. Anticarcinogenic effect of Panax ginseng C.A. Meyer and identification of active compounds. J Korean Med Sci. 2001;16(Suppl):S6-18. https://doi.org/10.3346/jkms.2001.16.S.S6
  3. Joo SS, Won TJ, Lee DI. Reciprocal activity of ginsenosides in the production of proinflammatory repertoire, and their potential roles in neuroprotection in vivo. Planta Med. 2005;71(5):476-81. https://doi.org/10.1055/s-2005-864145
  4. Lui JH, Sataba EJ. The ginsenosides of various ginseng plants and selected products. J Nat Prod. 1980;43:340-6. https://doi.org/10.1021/np50009a004
  5. Lim W, Mudge KW, Vermeylen F. Effects of population, age, and cultivation methods on ginsenoside content of wild American ginseng (Panax quinquefolium). J Agric Food Chem. 2005;53(22):8498-505. https://doi.org/10.1021/jf051070y
  6. Shin SS, Kim KC, Choi YH, Lee YT, Eom HS, Kim CS. Critic standardization and objectivity of mountain grown ginseng. KIOM. 2001;12:107-14.
  7. Kwon KR, Seo JC. Genetical identification of Korean wild ginseng and American wild ginseng by using pyrosequencing method. Kor J Herbology. 2004;19(4):45-50.
  8. Kwon KR, Park WP, Kang WM, Jeon EY, Jang JH. Identification and analysis of differentially expressed genes in mountain cultivated ginseng and mountain wild ginseng. J Acupunct Meridian Stud. 2011;4(2):123-8. https://doi.org/10.1016/S2005-2901(11)60018-6
  9. Diatchenko L, Lau YF, Campbell AP, Chenchik A, Moqadam F, Huang B, et al. Suppression subtractive hybridization: a method for generating differentially regulated or tissuespecific cDNA probes and libraries. Proc Natl Acad Sci USA. 1996;93(12):6025-30. https://doi.org/10.1073/pnas.93.12.6025
  10. Diatchenko L, Lukyanov S, Lau YF, Siebert PD. Suppression subtractive hybridization: a versatile method for identifying differentially expressed genes. Methods Enzymol. 1999;303:349-80. https://doi.org/10.1016/S0076-6879(99)03022-0
  11. Serino G, Maliga P. RNA polymerase subunits encoded by the plastid rpo genes are not shared with the nucleusencoded plastid enzyme. Plant Physiol. 1998;117(4):1165-70. https://doi.org/10.1104/pp.117.4.1165
  12. Mullet JE. Dynamic regulation of chloroplast transcription. Plant Physiol. 1993;103(2):309-13. https://doi.org/10.1104/pp.103.2.309
  13. Igloi GL, Kossel H. The transcriptional apparatus of chloroplasts. Critical Reviews in Plant Sciences. 1992;10(6):525-58. https://doi.org/10.1080/07352689209382326

피인용 문헌

  1. Intravenous Single-dose Toxicity of Mountain Ginseng Pharmacopuncture in Sprague-Dawley Rats vol.17, pp.3, 2014, https://doi.org/10.3831/KPI.2014.17.026
  2. Identification and Analysis of the Novel pGAPDH-w Gene Differentially Expressed in Wild Ginseng vol.16, pp.1, 2013, https://doi.org/10.3831/KPI.2013.16.002