Restorative Dentistry and Endodontics

The Korean Academy of Conservative Dentistry (대한치과보존학회)
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Morphological evaluation during in vitro chondrogenesis of dental pulp stromal cells

영구치 치수 기질세포를 이용한 연골 분화 및 분화 시기에 따른 형태학적 변화

  • Chung, Choo-Ryung (Department of Orthodontics, Yonsei University College of Dentistry) ;
  • Kim, Ha-Na (Department of Orthodontics, Yonsei University College of Dentistry) ;
  • Park, Yeul (Department of Orthodontics, Yonsei University College of Dentistry) ;
  • Kim, Min-Jeong (Department of Orthodontics, Yonsei University College of Dentistry) ;
  • Oh, Young-Ju (Department of Orthodontics, Yonsei University College of Dentistry) ;
  • Shin, Su-Jung (Department of Conservative Dentistry, Yonsei University College of Dentistry) ;
  • Choi, Yoon-Jeong (Department of Orthodontics, Yonsei University College of Dentistry) ;
  • Kim, Kyung-Ho (Department of Orthodontics, Yonsei University College of Dentistry)
  • 정주령 (연세대학교 치과대학 교정과학교실) ;
  • 김하나 (연세대학교 치과대학 교정과학교실) ;
  • 박열 (연세대학교 치과대학 교정과학교실) ;
  • 김민정 (연세대학교 치과대학 교정과학교실) ;
  • 오영주 (연세대학교 치과대학 교정과학교실) ;
  • 신수정 (연세대학교 치과대학 보존과학교실) ;
  • 최윤정 (연세대학교 치과대학 교정과학교실) ;
  • 김경호 (연세대학교 치과대학 교정과학교실)
  • Received : 2011.11.28
  • Accepted : 2012.02.02
  • Published : 2012.03.01
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Abstract

Objectives: The aim was to confirm the stem cell-like properties of the dental pulp stromal cells and to evaluate the morphologic changes during in vitro chondrogenesis. Materials and Methods: Stromal cells were outgrown from the dental pulp tissue of the premolars. Surface markers were investigated and cell proliferation rate was compared to other mesenchymal stem cells. Multipotency of the pulp cells was confirmed by inducing osteogenesis, adipogenesis and chondrogenesis. The morphologic changes in the chondrogenic pellet during the 21 day of induction were evaluated under light microscope and transmission electron microscope. TUNEL assay was used to evaluate apoptosis within the chondrogenic pellets. Results: Pulp cells were CD90, 105 positive and CD31, 34 negative. They showed similar proliferation rate to other stem cells. Pulp cells differentiated to osteogenic, adipogenic and chondrogenic tissues. During chondrogenesis, 3-dimensional pellet was created with multi-layers, hypertrophic chondrocyte-like cells and cartilage-like extracellular matrix. However, cell morphology became irregular and apoptotic cells were increased after 7 day of chondrogenic induction. Conclusions: Pulp cells indicated mesenchymal stem cell-like characteristics. During the in vitro chondrogenesis, cellular activity was superior during the earlier phase (within 7 day) of differentiation.

Keywords

References

  1. Okeson JP. Management of temporomandibular disorder 5th edition. Philadelphia: Elsevier; 2003. p 15-22.
  2. Arnett GW, Milam SB, Gottesman L. Progressive mandibular retrusion-idiopathic condylar resorption. Part II. Am J Orthod Dentofacial Orthop 1996;110:117-127. https://doi.org/10.1016/S0889-5406(96)70099-9
  3. Arnett GW, Milam SB, Gottesman L. Progressive mandibular retrusion-idiopathic condylar resorption. Part I. Am J Orthod Dentofacial Orthop 1996;110:8-15. https://doi.org/10.1016/S0889-5406(96)70081-1
  4. Wolford LM, Cardenas L. Idiopathic condylar resorption: diagnosis, treatment protocol, and outcomes. Am J Orthod Dentofacial Orthop 1999;116:667-677. https://doi.org/10.1016/S0889-5406(99)70203-9
  5. Crawford JG, Stoelinga PJ, Blijdorp PA, Brouns JJ. Stability after reoperation for progressive condylar resorption after orthognathic surgery: report of seven cases. J Oral Maxillofac Surg 1994;52:460-466. https://doi.org/10.1016/0278-2391(94)90341-7
  6. De Clercq CA, Neyt LF, Mommaerts MY, Abeloos JV, De Mot BM. Condylar resorption in orthognathic surgery: a retrospective study. Int J Adult Orthodon Orthognath Surg 1994;9:233-240.
  7. Merkx MA, Van Damme PA. Condylar resorption after orthognathic surgery. Evaluation of treatment in 8 patients. J Craniomaxillofac Surg 1994;22:53-58. https://doi.org/10.1016/S1010-5182(05)80297-5
  8. Mcllwraith CW, Frisbie DD, Rodkey WG, Kisiday JD, Werpy NM, Kawcak CE, Steadman JR. Evaluation of intra-articular mesenchymal stem cells to augment healing of microfractured chondral defects. Arthroscopy 2011;27:1552-1561. https://doi.org/10.1016/j.arthro.2011.06.002
  9. Meyerrose T, Olson S, Pontow S, Kalomoiris S, Jung Y, Annett G, Bauer G, Nolta JA. Mesenchymal stem cells for the sustained in vivo delivery of bioactive factors. Adv Drug Deliv Rev 2010;62:1167-1174. https://doi.org/10.1016/j.addr.2010.09.013
  10. van Buul GM, Kotek G, Wielopolski PA, Farrell E, Bos PK, Weinans H, Grohnert AU, Jahr H, Verhaar JA, Krestin GP, van Osch GJ, Bernsen MR. Clinically translatable cell tracking and quantification by MRI in cartilage repair using superparamagnetic iron oxides. PLoS One 2011;6:e17001. https://doi.org/10.1371/journal.pone.0017001
  11. Zhang J, Pan T, Im HJ, Fu FH, Wang JH. Differential properties of human ACL and MCL stem cells may be responsible for their differential healing capacity. BMC Med 2011;9:68. https://doi.org/10.1186/1741-7015-9-68
  12. Gronthos S, Mankani M, Brahim J, Robey PG, Shi S. Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc Natl Acad Sci U S A 2000;97:13625-13630. https://doi.org/10.1073/pnas.240309797
  13. Seo BM, Miura M, Gronthos S, Bartold PM, Batouli S, Brahim J, Young M, Robey PG, Wang CY, Shi S. Investigation of multipotent postnatal stem cells from human periodontal ligament. Lancet 2004;364:149-155. https://doi.org/10.1016/S0140-6736(04)16627-0
  14. Huang AH, Chen YK, Chan AW, Shieh TY, Lin LM. Isolation and characterization of human dental pulp stem/stromal cells from nonextracted crown-fractured teeth requiring root canal therapy. J Endod 2009; 35:673-681. https://doi.org/10.1016/j.joen.2009.01.019
  15. Pierdomenico L, Bonsi L, Calvitti M, Rondelli D, Arpinati M, Chirumbolo G, Becchetti E, Marchionni C, Alviano F, Fossati V, Staffolani N, Franchina M, Grossi A, Bagnara GP. Multipotent mesenchymal stem cells with immunosuppressive activity can be easily isolated from dental pulp. Transplantation 2005;80:836-842. https://doi.org/10.1097/01.tp.0000173794.72151.88
  16. Struys T, Moreels M, Martens W, Donders R, Wolfs E, Lambrichts I. Ultrastructural and immunocytochemical analysis of multilineage differentiated human dental pulp- and umbilical cord-derived mesenchymal stem cells. Cells Tissues Organs 2011;193:366-378. https://doi.org/10.1159/000321400
  17. Ichinose S, Muneta T, Koga H, Segawa Y, Tagami M, Tsuji K, Sekiya I. Morphological differences during in vitro chondrogenesis of bone marrow-, synovium-MSCs, and chondrocytes. Lab Invest 2010;90:210-221. https://doi.org/10.1038/labinvest.2009.125
  18. Huang GT, Sonoyama W, Chen J, Park SH. In vitro characterization of human dental pulp cells: various isolation methods and culturing environments. Cell Tissue Res 2006;324:225-236. https://doi.org/10.1007/s00441-005-0117-9
  19. Song SY, Jung JE, Jeon YR, Tark KC, Lew DH. Determination of adipose-derived stem cell application on photo-aged fibroblasts, based on paracrine function. Cytotherapy 2011;13:378-384. https://doi.org/10.3109/14653249.2010.530650
  20. Kim NR, Lee DH, Ahn SJ, Lee IS, Yang HC. The differentiation-inducing effect of conditioned media obtained from dental pulp cells. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2009;107:e54-59.
  21. Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D, Deans R, Keating A, Prockop Dj, Horwitz E. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy 2006;8:315-317. https://doi.org/10.1080/14653240600855905
  22. Ahrens PB, Solursh M, Reiter RS. Stage-related capacity for limb chondrogenesis in cell culture. Dev Biol 1977; 60:69-82. https://doi.org/10.1016/0012-1606(77)90110-5
  23. Miura M, Gronthos S, Zhao M, Lu B, Fisher LW, Robey PG, Shi S. SHED: stem cells from human exfoliated deciduous teeth. Proc Natl Acad Sci U S A 2003;100:5807-5812. https://doi.org/10.1073/pnas.0937635100
  24. Nam H, Lee G. Identification of novel epithelial stem cell-like cells in human deciduous dental pulp. Biochem Biophys Res Commun 2009;386:135-139. https://doi.org/10.1016/j.bbrc.2009.05.141
  25. Choi KM, Seo YK, Yoon HH, Song KY, Kwon SY, Lee HS, Park JK. Effects of mechanical stimulation on the proliferation of bone marrow-derived human mesenchymal stem cells. Biotechnology and Bioprocess Engineering 2007;12:601-609. https://doi.org/10.1007/BF02931075
  26. Han MJ, Seo YK, Yoon HH, Song KY, Park JK. Effect of mechanical tension on the human dental pulp cells. Biotechnology and Bioprocess Engineering 2008;13:410-417. https://doi.org/10.1007/s12257-008-0146-9
  27. Tanaka K, Iwasaki K, Feghali KE, Komaki M, Ishikawa I, Izumi Y. Comparison of characteristics of periodontal ligament cells obtained from outgrowth and enzymedigested culture methods. Arch Oral Biol 2011;56:380-388. https://doi.org/10.1016/j.archoralbio.2010.10.013
  28. Mobasheri A, Csaki C, Clutterbuck AL, Rahmanzadeh M, Shakibaei M. Mesenchymal stem cells in connective tissue engineering and regenerative medicine: applications in cartilage repair and osteoarthritis therapy. Histol Histopathol 2009;24:347-366.

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