미생물학회지 (Korean Journal of Microbiology)

  • 제48권1호
  • /
  • Pages.22-28
  • /
  • 2012
  • /
  • 0440-2413(pISSN)
  • /
  • 2383-9902(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지
DOI QR코드

DOI QR Code

경기인천 연안에서 분리된 장염비브리오균의 항생제 내성 및 플라스미드 보유 현황

Antibiotic Resistance and Plasmid Profile of Vibrio parahaemolyticus Strains Isolated from Kyunggi-Incheon Coastal Area

  • 한아름 (인천대학교 생명과학기술대학 생명공학부) ;
  • 윤영준 (인천대학교 생명과학기술대학 생명공학부) ;
  • 김정완 (인천대학교 생명과학기술대학 생명공학부)
  • 투고 : 2012.02.16
  • 심사 : 2012.03.16
  • 발행 : 2012.03.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

장염비브리오는 우리나라에서 하절기에 발생하는 식중독의 주요 원인균으로 주로 해수 및 어패류를 통해 인체에 감염된다. 최근 지구 온난화로 해수의 온도가 상승하면서 해양환경 중 세균들의 분포도가 증가하고 또 다양한 항생제에 대한 내성을 보이는 병원균들이 확산되고 있어 국민보건 및 수산양식 등의 분야에 큰 문제로 대두되고 있다. 이에 본 연구에서는 경기, 인천지역 7곳에서 해수 및 어패류 시료를 채취하여 장염비브리오균의 분포도를 모니터링하고 이들의 항생제 내성 양상을 분석하였다. 총 966건의 시료를 $CHROMagar^{TM}$ 및 TCBS 평판배지와 multiplex PCR기법으로 동정했을 때 전체 해수 시료의 61.9%, 어패류 시료의 41.8% 등 총 47.7%의 시료에서 장염비브리오균이 검출되었다. 이들 분리균주들의 항생제 내성을 13종의 항생제에 대해 분석한 결과 97.3%와 87.3%가 각각 vancomycin과 ampicillin에 대해 내성을 보였고 cephalothin (48.8%)과 rifampin (46.1%)에 대한 내성비율도 기타 다른 항생제들에 비해 상대적으로 높은 것으로 나타났다. 분리균주들의 항생제 감수성은 chloramphenicol (91.7%)과 trimethoprim-sulfamethoxazole (91.8%)에 대해 가장 높았고, gentamycin (82.3%), tobramycin (74.8%), nalidixic acid (71.6%), tetracyclin (69.4%), cefotaxime (63.0%) 등의 순서로 감수성 비율이 높았다. 분리균주들의 약 69%가 vancomycin과 ampicillin을 포함하여 3개 이상의 항생제에 대해 다중내성을 보였고 특히 11개 항생제에 대해 내성을 보이는 균주도 2개 검색되었다. 항생제 내성 장염비브리오 분리균주들의 플라스미드 프로파일을 분석한 결과 55.1%가 플라스미드를 보유하고 있었으며, 이들은 총 24가지 유형으로 분류되었으나 플라스미드 프로파일과 항생제 내성 간의 뚜렷한 상관관계는 관찰되지 않았다.

Vibrio parahaemolyticus is one of the major agents responsible for food poisoning during summer in Korea, which is transmitted via seawater or seafoods. Recently, distribution of the bacteria in the marine environment has been increased due to global warming. Great concern also has been raised regarding public hygiene as well as marine culture by the emergence of pathogens with antibiotic resistance. Therefore, distribution of V. parahaemolyticus and antibiotic resistance of the isolates were monitored in 7 coastal areas of Kyonggi Province and Incheon by sampling seawater, fishes and clams monthly. V. parahaemolyticus was detected from 47.7% of 966 samples (seawater 61.9%, seafoods 41.8%) analyzed using $CHROMagar^{TM}$ and TCBS agar plates as well as multiplex PCR. Among 13 antibiotics tested, resistance to vancomycin and ampicillin was observed in 97.3% and 87.3% of the isolates, respectively, and the ratios of them resistant to cephalothin (48.8%) and rifampin (46.1%) were also high. The isolates were most highly sensitive to chloramphenicol (91.7%) and trimethoprim-sulfamethoxazole (91.8%). The ratio of sensitivity for other antibiotics was also high in the descending order of gentamycin (82.3%), tobramycin (74.8%), nalidixic acid (71.6%), tetracyclin (69.4%), cefotaxime (63.0%). About 69% of the isolates showed multiple drug resistance toward 3 antibiotics including vancomycin and ampicillin. Two of them exhibited resistance for 11 antibiotics used in this study. Plasmid profile analysis of the isolates with antibiotic resistance revealed that 55.1% of them retained plasmids of 24 different types. However, no clear inter-relationship between the resistance and the plasmid profile has been observed.

키워드

참고문헌

  1. Akinbowale, O.L., Peng, H., and Barton, M.D. 2006. Antimicrobial resistance in bacteria isolated from aquaculture sources in Australia. J. Appl. Microbiol. 100, 1103-1113. https://doi.org/10.1111/j.1365-2672.2006.02812.x
  2. Alagappan, K.M., Devivasigamani, B., Somasundaram, S.T., and Kumaran, S. 2010. Occurance of Vibrio parahaemolyticus and its specific phages from shrimp ponds in east coast of India. Curr. Microbiol. 61, 235-240. https://doi.org/10.1007/s00284-010-9599-0
  3. Aoki, T. and Takahashi, A. 1987. Class D tetracycline resistance determinants of R-plasmids from fish pathogens Aeromonas hydrophila, Edwardsiella tarda and Pasteurella piscisida. Antimicrob. Agents Chemother. 31, 1278-1280. https://doi.org/10.1128/AAC.31.8.1278
  4. Bauer, H.W., Kirby, W.M.M., Sherris, J.C., and Turk, M. 1966. Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clin. Pathol. 45, 493-496. https://doi.org/10.1093/ajcp/45.4_ts.493
  5. Chong, Y. and Lee, K. 1998. Antimicrobial susceptibility of bacteria isolated from Korean patients. Microb. Ind. 24, 1-11.
  6. Chung, Y.H., Jeoung, H.H., Kwon, Y.I., and Kim, H.R. 2006. The monitoring of antibiotic resistant bacteria from environment (River and Downstream). Ann. Report KFDA. 10, 1022-1023.
  7. Davies, J.E. 1997. Origins, acquisition and dissemination of antibiotic resistance determinants. Ciba F. Symp. 207, 15-27; discussion 27-35.
  8. Devie, R., Surendran, P.K., and Chakraborty, K. 2009. Antibiotic resistance and plasmid profiling of Vibrio parahaemolyticus isolated from shrimp farms along the southwest coast of India. World J. Microbiol. Biotechnol. 25, 2005-2012. https://doi.org/10.1007/s11274-009-0101-8
  9. Fujino, T., Okuno, Y., Nakada, D., Aoyama, A., Fukai, K., Mukai, T., and Ueho, T. 1953. On the bacteriological examination of shirasu food poisoning. Med. J. Osaka Univ. 4, 299-304.
  10. Han, F., Walker, R.D., Janes, M.E., Prinyawiwatkul, W., and Ge, B. 2007. Antimicrobial susceptibilities of Vibrio parahaemolyticus and Vibrio vulnificus isolates from Louisiana Gulf and retail raw oysters. Appl. Environ. Microbiol. 73, 7096-7098. https://doi.org/10.1128/AEM.01116-07
  11. Hwang, I.G., Kwak, H.S., Kim, M.K., Han, J.A., Park, Y.C., Kim, E.J., Lee, G.Y., Yoon, H.S., Lee, J.S., Cho, J.H., and et al. 2008. Monitoring and evaluation of antimicrobial resistance of foodborne pathogenic bacteria. Ann. Report KFDA. 12, 191-192.
  12. Joseph, S.W., DeBell, R.M., and Brown, W.P. 1978. In vitro response to chloramphenicol, tetracycline, ampicillin, gentamicin, and beta-lactamase production by halophilic vibrios from human and environmental sources. Antimicrob. Agents Chemother. 13, 244-248. https://doi.org/10.1128/AAC.13.2.244
  13. Kitiyodom, S., Khemtong, S., Wongtavatchai, J., and Chuanchuen, R. 2010. Characterization of antibiotic resistance in Vibrio spp. isolated from farmed marine shrimps (Penaeus monodon). FEMS Microbiol. Lett. Ecol. 72, 219-227. https://doi.org/10.1111/j.1574-6941.2010.00846.x
  14. Kuhl, S.A., Pattee, P.A., and Baldwin, N.J. 1978. Chromosomal map location of the methicillin resistance determinant in Staphylococcus aureus. J. Bacteriol. 135, 460-465.
  15. Kumar, P.A., Patterson, J., and Karpagam, P. 2009. Multiple antibiotic resistance profiles of Vibrio choleae non-O1 and non-O139. Jpn. J. Infect. Dis. 62, 230-232.
  16. Lee, T.S. 2005. Establishment of antimicrobials management systems for aquaculture. Ann. Report KFDA. 9, 422-423.
  17. Lee, K. and Park, P. 2010. Antibiotic-resistance profiles and the identification of the ampicillin-resistance gene of Vibrio parahaemolyticus isolated from Seawater. Kor. J. Fish Aquat. Sci. 43, 637-641. https://doi.org/10.5657/kfas.2010.43.6.637
  18. Li, J.J., Yie, R., Foo, W.T., Ling, J.M.L., Xu, H., and Woo, N.Y.S. 1999. Antibiotic resistance and plasmid profiles of vibrio isolates from cultured silver sea bream, Sparus sarba. Mar. Pollt. Bull. 39, 245-249. https://doi.org/10.1016/S0025-326X(99)00062-4
  19. Martinez, J.L. and Baquero, F. 2000. Mutation frequencies and antibiotic resistance. Antimicrob. Agents Chemother. 44, 1771-1777. https://doi.org/10.1128/AAC.44.7.1771-1777.2000
  20. Mudryk, Z. 2005. Occurrence and distribution antibiotic resistance of heterotrophic bacteria isolated from a marine beach. Mar. Pollut. Bull. 50, 80-86. https://doi.org/10.1016/j.marpolbul.2004.10.001
  21. Rowe-Magnus, D.A. and Mazel, D. 2002. The role of integrons in antibiotic resistance gene capture. Int. J. Med. Microbiol. 292, 115-125. https://doi.org/10.1078/1438-4221-00197
  22. Sambrook, J., Fritsch, E.F., and Maniatis, T. 1989. Molecular cloning : A laboratory manual, pp. 142-152. Cold Spring Harbor Laboratory Press, USA.
  23. Sanjeev, S. 1999. Incidence, enteropathogenicity and antibiotic sensitivity of V. parahaemolyti-cus from a brackish water culture pond. Fish. Technol. 36, 13-18.
  24. Schwartz, T., Kohenen, W., Jansen, B., and Obst, U. 2003. Detection of antibiotic resistant bacteria and their resistant genes in wastewater, surface water, and drinking water biofilms. FEMS Microbiol. Ecol. 43, 325-335. https://doi.org/10.1111/j.1574-6941.2003.tb01073.x
  25. Shanthini, C.F., Kumar, P.A., and Patterson, J. 2004. Incidence and antibiotic susceptibility of Vibrio parahaemolyticus from sea foods of Tuticorin. Indian J. Fish. 51, 43-47.
  26. Shirai, H., Ito, H., Hirayama, T., Nakabayashi, Y., Kumagai, K., Takeda, Y., and Nishibuchi, M. 1990. Molecular epidemiologic evidence for association of thermostable direct hemolysin (TDH) and TDH-related hemolysin of Vibrio parahaemolyticus with gastroenteritis. Infect. Immun. 58, 3568-3573.
  27. Son, J.C., Park, S.W., and Min, K.J. 2003. Environmental and antimicrobial characteristics of Vibrio spp. isolated from fish, shellfish, seawater and brackish water samples in Gyeongbuk Eastern Coast. Kor. J. Environ. Hlth. 29, 94-102.
  28. Taniguchi, H., Hirano, H., Kubomura, S., Higashi, K., and Mizuguchi, Y. 1986. Comparison of the nucleotide sequences of the genes for the thermostable direct hemolysin and the thermolabile hemolysin from Vibrio parahaemolyticus. Microb. Pathog. 1, 425-432. https://doi.org/10.1016/0882-4010(86)90004-5
  29. Yoon, Y.J., Kim, D.Y., Lee, U.Y., Koh, Y.H., Kim, S.K., and Kim, J.W. 2000. Isolation and identification of Vibrio species contaminated in imported frozen seafoods. J. Food Hyg. Safety 15, 128-136.

피인용 문헌

  1. Antibiotic Susceptibility of Vibrio spp. Isolated from West Sea vol.49, pp.2, 2013, https://doi.org/10.7845/kjm.2013.3028
  2. Food-borne outbreaks, distributions, virulence, and antibiotic resistance profiles of Vibrio parahaemolyticus in Korea from 2003 to 2016: a review vol.21, pp.1, 2018, https://doi.org/10.1186/s41240-018-0081-4
  3. Characterization of Vibrio parahaemolyticus isolated from oysters in Korea: Resistance to various antibiotics and prevalence of virulence genes vol.118, pp.1-2, 2017, https://doi.org/10.1016/j.marpolbul.2017.02.070
  4. Antimicrobial-resistance Profiles and Virulence Genes of Vibrio parahaemolyticus Isolated from Seawater in the Wando Area vol.47, pp.3, 2014, https://doi.org/10.5657/KFAS.2014.0220
  5. Production of Monoclonal Antibodies against Vibrio parahaemolyticus and Development of High Sensitive Immuno-Selective Filtration Method vol.31, pp.1, 2016, https://doi.org/10.13103/JFHS.2016.31.1.21
  6. Antimicrobial Resistance and Minimum Inhibitory Concentrations of Vibrio parahaemolyticus Strains Isolated from Gomso Bay, Korea vol.49, pp.5, 2016, https://doi.org/10.5657/KFAS.2016.0582
  7. Antimicrobial susceptibility of Vibrio alginolyticus isolated from oyster in Korea vol.23, pp.20, 2016, https://doi.org/10.1007/s11356-016-7426-2
  8. Prevalence and antimicrobial susceptibility of Vibrio parahaemolyticus isolated from oysters in Korea vol.23, pp.1, 2016, https://doi.org/10.1007/s11356-015-5650-9
  9. 제주지역 양식 넙치(Paralichthys olivaceus)에서 분리한 어병세균 내 Erythromycin 내성 유전자 분석 vol.51, pp.4, 2012, https://doi.org/10.5657/kfas.2018.0397
  10. Genetic Relationship, Virulence Factors, Drug Resistance Profile and Biofilm Formation Ability of Vibrio parahaemolyticus Isolated From Mussel vol.10, pp.None, 2019, https://doi.org/10.3389/fmicb.2019.00513
  11. 2013-2016년 경남 연안 해수 및 패류에서 병원성 비브리오균의 계절적 및 지역적 변동 vol.52, pp.1, 2012, https://doi.org/10.5657/kfas.2019.0027