DOI QR코드

DOI QR Code

옻나무 추출물의 침지가 가스조절포장으로 저장한 한우고기 스테이크의 총환원력, 단백질산화 및 육색소산화에 미치는 영향

Effect of Immersion in Rhus verniciflua Stokes Extract on the Total Reducing Ability, Protein Oxidation and Myoglobin Oxidation of Hanwoo Beef Steak Stored with Modified-atmosphere Packaging

  • 강선문 (농촌진흥청 국립축산과학원) ;
  • 송영한 (강원대학교 동물생명시스템학과) ;
  • 정천순 (강원대학교 원예학과) ;
  • 조수현 (농촌진흥청 국립축산과학원) ;
  • 박범영 (농촌진흥청 국립축산과학원) ;
  • 정석근 (농촌진흥청 국립축산과학원) ;
  • 이성기 (강원대학교 동물식품응용과학과)
  • Kang, Sun-Moon (National Institute of Animal Science, Rural Development Administration) ;
  • Song, Young-Han (Department of Animal Life System, Kangwon National University) ;
  • Jeong, Cheon-Soon (Department of Horticulture, Kangwon National University) ;
  • Cho, Soo-Hyun (National Institute of Animal Science, Rural Development Administration) ;
  • Park, Beom-Young (National Institute of Animal Science, Rural Development Administration) ;
  • Jung, Seok-Geun (National Institute of Animal Science, Rural Development Administration) ;
  • Lee, Sung-Ki (Department of Animal Products and Food Science, Kangwon National University)
  • 투고 : 2012.04.20
  • 심사 : 2012.05.25
  • 발행 : 2012.06.30

초록

본 연구는 옻나무 추출물(Rhus verniciflua Stokes extract, RVSE)의 침지가 가스조절포장(modified-atmosphere packaging, MAP)으로 저장한 한우고기 스테이크의 총환원력, 단백질산화 및 육색소산화에 미치는 영향을 구명하고자 실시하였다. 시료(Hanwoo longissimus muscle)는 옻나무 추출물의 침지 여부에 따라 처리한 후 high oxygen-MAP(HOMAP; 75% $O_2$+20% $CO_2$+5% $N_2$)와 low oxygen-MAP (LOMAP; 0% $O_2$+20% $CO_2$+80% $N_2$)으로 포장하여 $4^{\circ}C$에서 9일 동안 저장하였다. 총환원력은 RVSE의 침지에 의해 증가되었으며(p<0.05), LOMAP에 의해 저장기간 동안 높게 유지되었다. Carbonyl 함량은 RVSE의 침지와 LOMAP에 의해 억제되었다. Metmyoglobin의 축적은 RVSE의 침지에 의해 증가되었으나, HOMAP에 의해 낮게 유지되었다. 이와 반대로 oxymyoglobin에 의한 적색 강도의 지표인 R630-R580은 RVSE의 침지에 의해 감소되었던 반면, HOMAP에 의해 높게 유지되었다. 명도($L^*$), 적색도($a^*$) 및 chroma($C^*$)는 RVSE의 침지에 의해 감소되었으나, HOMAP에 의해 높게 유지되었다. 반면에 hue-angle($H^{\circ}$)은 RVSE의 침지에 의해 증가되었으나, HOMAP에 의해 낮게 유지되었다. 따라서 RVSE의 침지는 한우고기 스테이크의 총환원력과 단백질산화 안정성을 증가시켰으나, 육색소산화 안정성 감소시켰다. 또한 HOMAP는 총환원력과 단백질산화 안정성을 감소시켰으나, 육색소산화 안정성은 증가시켰다.

The objective of this research was to investigate the effect of immersion in Rhus verniciflua Stokes extract (RVSE) on the total reducing ability (TRA), protein oxidation and myoglobin oxidation of beef steak (Hanwoo longissimus muscle) stored with high oxygen-modified-atmosphere packaging (MAP) (HOMAP, 75% $O_2$+20% $CO_2$+5% $N_2$) and low oxygen-MAP (LOMAP, 0% $O_2$+20% $CO_2$+80% $N_2$) at $4^{\circ}C$ for nine days. RVSE induced TRA (p<0.05), metmyoglobin (MetMb) formation, and the CIE $H^{\circ}$ value but reduced the carbonyl content and R630-R580, as an index of the intensity of redness by oxymyoglobin, and the CIE $L^*$,$a^*$, and $C^*$ values. HOMAP maintained a lower TRA, MetMb concentration, and CIE $H^{\circ}$ value but had higher R630-R580 and CIE $L^*$, $a^*$, and $C^*$ values compared to LOMAP. Therefore, RVSE induced TRA and protein oxidation stability but reduced myoglobin stability in Hanwoo beef steak. In addition, the effects of HOMAP were opposite those of RVSE.

키워드

참고문헌

  1. Kim MJ, Hyun JO (1997) Genetic variation in urushiol components of Rhus verniciflua Stokes. Korean J Breed, 29, 115-123
  2. Shin MK (1986) Coloured Limsangbonchohak. Namsandang, Seoul, Korea, p 165-718
  3. Lee JC, Lim KT, Jang YS (2002) Identification of Rhus verniciflua Stokes compounds that exhibit free radical scavenging and anti-apoptotic properties. Biochim Bioph Acta, 1570, 181-191 https://doi.org/10.1016/S0304-4165(02)00196-4
  4. Son YO, Lee KY, Lee JC, Jang HS, Kim JG, Jeon YM, Jang YS(2005) Selective antiproliferative and apoptotic effects of flavonoids purified from Rhus verniciflua Stokes on normal versus transformed hepatic cell lines. Toxicol Let, 155, 115-125 https://doi.org/10.1016/j.toxlet.2004.09.003
  5. Rice-Evans CA, Miller NJ, Paganga G (1996) Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Rad Biol & Med, 20, 933-956 https://doi.org/10.1016/0891-5849(95)02227-9
  6. Lim KT, Shim JH (1997) Antioxidative effects of ethanol extracts from Rhus verniciflua Stokes (RVS) on mouse whole brain cells. Korean J Food Sci Technol, 29, 1248-1254
  7. Kim DW, Liang CY, Kim YS, Kang CG, Lee SK (2003) Effects of addition of extracted Rhus verniciflua Stokes and supplementation on the meat quality of meat. Annal Anim Resour Sci, 14, 34-41
  8. Lee SK, Kang SM, Kim YS, Kang CG (2005) Quality comparison of emulsion-type sausage made from Rhus verniciflua Stokes fed pork and extract. Korean J Food Sci Ani Resour, 25, 210-217
  9. McMillin KW, Huang NY, Ho CP, Smith BS (1999) Quality and shelf-life of meat in case-ready modified atmosphere packaging. In: Quality Attributes in Muscle Foods, Xiong YL, Shahidi F, Ho CT (Editors), ACS Symposium Series, Plenum Publishing Co, NY, USA, p 73-93
  10. Martinez L, Djenane D, Cilla I, Beltran JA, Roncales P (2006) Effect of varying oxygen concentrations on the shelf-life of fresh pork sausages packaged in modified atmosphere. Food Chem, 94, 219-225 https://doi.org/10.1016/j.foodchem.2004.11.007
  11. Sorheim O, Ofstad R, Lea P (2004) Effect of carbon dioxide on yield, texture and microstructure of cooked ground beef. Meat Sci, 67, 231-236 https://doi.org/10.1016/j.meatsci.2003.10.010
  12. Silliker JH, Woodruff, RE, Lugg, JR, Wolfe SK, Brown WD (1977) Preservation of refrigerated meats with controlled atmospheres: Treatment and post-treatment effects of carbon dioxide on pork and beef. Meat Sci, 1, 195-201 https://doi.org/10.1016/0309-1740(77)90037-7
  13. Sorheim O, Aune T, Nesbakken T (1997) Technological, hygienic and toxicological aspects of carbon monoxide used in modified-atmosphere packaging of meat. Tr Food Sci Technol, 8, 307-312 https://doi.org/10.1016/S0924-2244(97)01062-5
  14. Ordonez JA, Ledward DA (1977) Lipid oxidation and myoglobin oxidation in pork stored in oxygen- and carbon dioxide-enriched atmospheres. Meat Sci, 1, 41-48. https://doi.org/10.1016/0309-1740(77)90030-4
  15. Lynch NM, Kastner CL, Kropf DH (1986) Consumer acceptance of vacuum packaged ground beef as influenced by product color and educational materials. J Food Sci, 51, 253-255, 272 https://doi.org/10.1111/j.1365-2621.1986.tb11102.x
  16. Kanner J (1994) Oxidative processes in meat and meat products: quality implications. Meat Sci, 36, 169-189 https://doi.org/10.1016/0309-1740(94)90040-X
  17. Xiong YL (2000) Protein oxidation and implications for muscle food quality. In: Antioxidants in Muscle Foods: Nutritional Strategies to Improve Quality, Decker EA, Faustman C, Lopez-Bote CJ (Editors), John Wiley & Sons, Inc, NY, USA, p 85-111
  18. Smith GC, Belk KE, Sofos JN, Tatum JD, Williams SN (2000) Economic implications of improved color stability in beef. In: Antioxidants in Muscle Foods: Nutritional Strategies to Improve Quality, Decker EA, Faustman C, Lopez-Bote CJ (Editors), John Wiley & Sons, Inc, NY, USA, p 397-426
  19. Kim YS, Liang CY, Kim HJ, Lee, SK (2004) Effects of chitosan dipping treatments with different molecular weights on the meat quality of Hanwoo (Korean cattle) beef duringrefrigerated storage. Korean J Food Sci Ani Resour, 24, 1-7
  20. Lee M, Cassens RG, Fennema OR (1981) Effect of meat ions on residual nitrite. J Food Proc Preservat, 5, 191-205 https://doi.org/10.1111/j.1745-4549.1981.tb00634.x
  21. Mercier Y, Gatellier P, Renerre M (2004) Lipid and protein oxidation in vitro, and antioxidant potential in meat from Charolais cows finished on pasture or mixed diet. Meat Sci, 66, 467-473 https://doi.org/10.1016/S0309-1740(03)00135-9
  22. Culler RD, Parrish FC, Smith GC, Cross HR (1978) Relationship of myofibril fragmentation index to certain chemical, physical and sensory characteristics of bovine longissimus muscle. J Food Sci, 43, 1177-1180 https://doi.org/10.1111/j.1365-2621.1978.tb15263.x
  23. Gornall AG, Bardawill CJ, David MM (1948) Determination of serum protein by means of the biuret reaction. J Biol Chem, 177, 751-766
  24. Krzywicki K (1979) Assessment of relative content of myoglobin, oxymyoglobin and metmyoglobin at the surface of the beef. Meat Sci, 3, 1-10 https://doi.org/10.1016/0309-1740(79)90019-6
  25. Demos B P, Gerrard DE, Mandigo RW, Gao X, Tan J (1996) Mechanically recovered neck bone lean and ascorbic acid improve color stability of ground beef patties. J Food Sci, 61, 656 -659 https://doi.org/10.1111/j.1365-2621.1996.tb13180.x
  26. Strange EE, Benedicts RC, Gugger RE, Metzger VG, Swift CE (1974) Simplified methodology for measuring meat color. J Food Sci 39, 988-992 https://doi.org/10.1111/j.1365-2621.1974.tb07293.x
  27. CIE (2004) Technical report: Colorimetry. 3rd ed, Commision Internationale de Leclairage Central Bureau, Vienna, Austria, Publication 8
  28. SPSS Inc (2005) SPSS 14.0 for Windows Evaluation Version, Statistical Package for the Social Sciences Incorporated, Illinois, USA
  29. Kang SM (2011) Effects of Rhus vernicifluaStokes extract and modified atmosphere packaging (MAP) on the oxidative characteristics of Hanwoo (Korean cattle) beef. PhD thesis, Kangwon National Univ, Korea
  30. Zakrys PI, Hogan SA, O'Sullivan MG, Allen P, Kerry JP (2008) Effects of oxygen concentration on the sensory evaluation and quality indicators of beef muscle packed under modified atmosphere. Meat Sci, 79, 648-655 https://doi.org/10.1016/j.meatsci.2007.10.030
  31. Chan KM, Decker EA, Faustman C (1994) Endogenous skeletal muscle antioxidants. Crit Rev Food Sci Nutr, 34, 403-426 https://doi.org/10.1080/10408399409527669
  32. Cheigh HS (1994) Lipid peroxidation and its nutritional significance. J Korean Soc Food Nutr, 23, 867-878
  33. Stadtman ER, Oliver CN (1991) Metal-catalyzedoxidation of proteins. J Biol Chem, 266, 2005-2008
  34. Lund MN, Hviid MS, Skibsted LH (2007) The combined effectof antioxidants and modified atmosphere packaging on protein and lipid oxidation in beef patties during chill storage. Meat Sci, 27, 226-233
  35. Estevez M, Cava R (2004) Lipid and protein oxidation, release of iron from heme molecule and colour deterioration during refrigerated storage of liver pate. Meat Sci, 68, 551-558 https://doi.org/10.1016/j.meatsci.2004.05.007
  36. Liang CY, Kang SM, Kim YS, Lee SK (2005) Antioxidant activity of Rhus venicifluaStokes extract in model systems and cooked beef. Korean J Food Sci Ani Resour, 25, 189-195
  37. Koohmaraie M, Geesink GH (2006) Contribution of postmortemmuscle biochemistry to the delivery of consistent meat quality with particular focus on the calpain system. Meat Sci, 74, 34-43 https://doi.org/10.1016/j.meatsci.2006.04.025
  38. Lund MN, Lametsch R, Hviid M, Jensen ON, Skibsted LH (2007) High-oxygen packaging atmosphere influences protein oxidation and tenderness of porcine longissimus dorsi during chill storage. Meat Sci, 77, 295-303 https://doi.org/10.1016/j.meatsci.2007.03.016
  39. Faustman C, Cassens RG (1990) The biochemical basis for discoloration in fresh meat: A review. J Muscle Foods, 1, 217-243 https://doi.org/10.1111/j.1745-4573.1990.tb00366.x
  40. O'Grady MN, Monahan FJ, Bailey J, Allen P, Buckley DJ, Keane MG (1998) Colour-stabilising effect of muscle vitamin E in minced beef stored in high oxygen packs. Meat Sci, 50, 73-80 https://doi.org/10.1016/S0309-1740(98)00017-5
  41. Faustman C, Sun Q, Mancini R, Suman SP (2010) Myoglobin and lipid oxidation interactions: mechanistic bases and control. Meat Sci, 86, 86-94 https://doi.org/10.1016/j.meatsci.2010.04.025
  42. Sanchez-Escalante A, Djenane D, Torrescano G, Beltran JA, Roncales P (2001) The effects of ascorbic acid, taurine, carnosine and rosemary powder on colour and lipid stability of beef patties packaged in modified atmosphere. Meat Sci, 58, 421-429 https://doi.org/10.1016/S0309-1740(01)00045-6
  43. Hayes JE, Stepanyan V, Allen P, O'Grady MN, O'Brien NM, Kerry JP (2009) The effect of lutein, sesamol, ellagicacid and olive leaf extract on lipid oxidation and oxymyoglobin oxidation in bovine and porcine muscle model system. Meat Sci, 83, 201-208 https://doi.org/10.1016/j.meatsci.2009.04.019
  44. Halliwell B, Gutteridge JMC (1986) Oxygen free radicals and iron in relation to biology and medicine: Some problems and concepts. Arch Biochem Biophys, 246, 501-514 https://doi.org/10.1016/0003-9861(86)90305-X
  45. Lee SK, Kim YS, Liang CY, Song YH (2003) Effects of dietary vitamin E supplementation on color stability, lipid oxidation and reducing ability of Hanwoo (Korean cattle) beef during retail display. Asian-Aust J Anim Sci, 16, 1529-1534 https://doi.org/10.5713/ajas.2003.1529
  46. Hayes JE, Stepanyan V, Allen P, O'Grady MN, Kerry JP (2010) Effect of lutein, sesamol, ellagic acid and olive leaf extract on the quality and shelf-life stability of packaged raw minced beef patties. Meat Sci, 84, 613-620 https://doi.org/10.1016/j.meatsci.2009.10.020