Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
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Factors to Predict Successful Harvest during Autologous Peripheral Hematopoietic Stem Cell Collection

  • Kim, Mun-Ja (Hematopoietic Stem Cell Bank, Yeungnam University Medical Center) ;
  • Jin, Soo-He (Department of Preventive Medicine, School of Medicine, Kyungpook National University) ;
  • Lee, Duk-Hee (Department of Preventive Medicine, School of Medicine, Kyungpook National University) ;
  • Park, Dae-Weon (Hematopoietic Stem Cell Bank, Yeungnam University Medical Center) ;
  • Koh, Sung-Ae (Department of Internal Medicine, Yeungnam University, College of Medicine) ;
  • Lee, Kyung-Hee (Department of Internal Medicine, Yeungnam University, College of Medicine) ;
  • Hyun, Myung-Soo (Department of Internal Medicine, Yeungnam University, College of Medicine) ;
  • Kim, Min-Kyoung (Department of Internal Medicine, Yeungnam University, College of Medicine)
  • Received : 2012.05.17
  • Accepted : 2012.06.25
  • Published : 2012.06.30
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Abstract

Autologous peripheral blood stem cell transplantation (PBSCT) has been used as a major treatment strategy for hematological malignancies. The number of CD34 positive cells in the harvested product is a very important factor for achieving successful transplantation. We studied the factors that can predict the number of CD34 positive cells in the harvested product of acute myelocytic leukemia (AML), multiple myeloma (MM) and Non-Hodgkin's lymphoma (NHL) patients after mobilizing them with chemotherapy plus G-CSF. A total of 73 patients (AML 19 patients, MM 28 patients, NHL 26 patients) with hematological malignancies had been mobilized with chemotherapy and granulocyte colony-stimulating growth factor from April, 2000 to February, 2012. Group's characteristics, checkup opinion of pre-peripheral blood on the day of harvest & outcome of PBSC were analyzed and evaluated using SPSS statistics program after grouping patients as below; group 1: CD34 cell counts < $2{\times}10^6/kg$ (n=16); group 2: $2{\times}10^6/kg{\leq}CD34$ cell counts < $6{\times}10^6/kg$ (n=32); group 3: CD34 cell counts ${\geq}6{\times}10^6/kg$ (n=25). We analyzed the clinical characteristics, the peripheral blood (PB) parameters and the number of CD34 positive cells in the PB and their correlation with the yield of CD34 positive cells collected from the mobilized patients. The total number of leukapheresis sessions was 263 (mean: 3.55 session per patient), and the mean number of harvested CD34 positive cells per patient was $7.37{\times}10^6/kg$. The number of CD34 positive cells in product was significantly correlated with the number of platelet and CD34 positive cells in peripheral blood (P<0.05). The number of PB CD34 positive cells was the best significant factor for the quantity of harvested CD34 positive cells on the linear regression analysis (P<0.05). Many factors could influence the mobilization of peripheral blood stem cells. Platelet count and PB CD34 positive cells count were the two variables which remained to be significant in multivariate analysis. Therefore, the number of platelet and CD34 positive cells in peripheral blood on the day of harvest can be used as an accurate predictor for successful peripheral blood stem cell collection.

Keywords

References

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