Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effects of Heat Treatment and Extraction Method on Antioxidant Activity of Several Medicinal Plants

열처리와 추출방법에 따른 몇 가지 약초의 항산화 활성

  • Jang, Gwi-Yeong (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Kim, Hyun-Young (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Lee, Sang-Hoon (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Kang, Yu-Ri (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Hwang, In-Guk (Dept. of Agrofood Resources, NAAS) ;
  • Woo, Koan-Sik (Dept. of Functional Crop, NICS) ;
  • Kang, Tae-Soo (Dept. of Biofood Science and Biotechnology, Chungbuk Provincial University of Science and Technology) ;
  • Lee, Jun-Soo (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Jeong, Heon-Sang (Dept. of Food Science and Technology, Chungbuk National University)
  • 장귀영 (충북대학교 식품공학과) ;
  • 김현영 (충북대학교 식품공학과) ;
  • 이상훈 (충북대학교 식품공학과) ;
  • 강유리 (충북대학교 식품공학과) ;
  • 황인국 (국립농업과학원 농식품자원부) ;
  • 우관식 (국립식량과학원 기능성작물부) ;
  • 강태수 (충북도립대학 바이오식품생명과학과) ;
  • 이준수 (충북대학교 식품공학과) ;
  • 정헌상 (충북대학교 식품공학과)
  • Received : 2012.03.12
  • Accepted : 2012.05.14
  • Published : 2012.07.31
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Abstract

We investigated the effects of heat treatment and extraction method on the antioxidant activities of five medicinal plants: Cyperus rotundus, Eucommia ulmoides, Bupleurum falcatum, Achyranthes japonica Nakai, and Akebia quinata. Extraction was performed with only ultrasound, ultrasound after heating at $130^{\circ}C$ for 2 hours, and reflux extraction with distilled water. The phenolic contents of reflux extraction and ultrasound extraction after heating were higher than only ultrasound extraction, and ultrasound extraction after heating samples was higher than reflux extraction except for Eucommia ulmoides and Cyperus rotundus. Total flavonoid content was higher in reflux and ultrasound extraction after heating samples than only ultrasound extraction, except for Cyperus rotundus. ABTS radical scavenging activity was higher in reflux extraction and ultrasound extraction after heating a sample, than only ultrasound extraction. DPPH radical scavenging activity was higher in reflux extraction except for Achyranthes japonica Nakai and Akebia quinata. The reducing power of ultrasound extraction after heating was higher with Achyranthes japonica Nakai. From the results of this study, we can expect to increase the antioxidant activity of medicinal plant extracts by applying suitable extraction and pretreatment conditions on the type of medicinal plant.

추출방법에 따른 몇 가지 약초의 항산화 활성 변화를 연구하였다. 총 5가지 약초(우슬, 목통, 두충, 시호, 향부자)에 대해 비가열 초음파추출, 환류추출 및 열처리 초음파추출을 실시하였다. 총 폴리페놀 함량은 환류추출과 열처리 초음파 추출이 비가열 초음파추출보다 높았으며, 두충과 향부자를 제외한 세 시료가 열처리 초음파추출이 환류추출보다 높았다. 총 플라보노이드 함량은 향부자를 제외한 네 시료가 열처리 초음파추출과 환류추출이 비가열 초음파추출보다 더 많았으며, 우슬과 목통을 제외한 세 시료에서 환류추출이 열처리 초음파추출보다 더 많았다. ABTS 라디칼 소거능은 환류추출과 열처리 초음파추출이 비가열 초음파추출보다 작았으며, DPPH 라디칼 소거능은 우슬과 목통을 제외한 세 시료는 환류추출에서 더 높은 활성을 나타내었다. 환원력은 우슬이 가열처리 시 많은 증가를 나타내었으며, 다른 네 시료는 추출방법에 따른 차이가 작았다. 본 실험 결과에 따르면, 약초의 종류에 따라 적합한 추출방법과 전처리를 적용함으로써 높은 항산화 활성을 가진 추출물을 얻는 것이 가능할 것이라 판단된다.

Keywords

References

  1. Reiter RJ. 1995. Oxidative process and antioxidative defense mechanism in the aging brain. FASEB J 9: 526-533. https://doi.org/10.1096/fasebj.9.7.7737461
  2. Ode P. 1995. Herbal insights-a clpse look at active constituents of medicinal herbs. SOFW J 121: 8-11.
  3. Choe SY, Yang KH. 1982. Toxicological studies of antioxidants butylated hydroxyl toluene (BHT) and butylated hydrozyanisole (BHA). Korean J Food Sci Technol 14: 283-288.
  4. Ryu HS, Kim HS. 2006. Effect of Sorghum bicolor L. moench (sorghum, su-su) water extracts on mouse immune cell activation. Korean J Food Nutr 19: 176-182.
  5. Shimomura H, Sashida Y, Nakata H. 1981. Palnt growth regulating activities of crude drugs and medicinal plants. Shoyakugaku Zasshi 35: 176-179.
  6. Kiso Y, suzuki Y, Konno C, Hkino H, Hashimoto I, Yagi Y. 1982. Liver-protective drugs. 3. The validity of the oriental medicines. 38. Application of carbon tetrachloride- induced liver lesion in mice for screening of liver protective crude drugs. Shoyakugaku Zasshi 36: 238-244.
  7. Kim JC, Choi GJ, Lee SW, Kim JS, Chung KY, Cho KY. 2004. Screening extracts of Achyranthes japonica and Rumex crispis for activity against various plant pathogenic fungi and control of powdery mildew. Pest Manag Sci 60: 803-808. https://doi.org/10.1002/ps.811
  8. Son KH, Hwang JH, Lee SH, Park JH, Kang SJ, Chang SY, Lee KS. 1999. Isolation and quantitative determination of 20-hydroxyecdysone from Achyranthes radiz. Korean J Pharmacogn 30: 335-339.
  9. Jung HJ, Lee CO, Lee KT, Park HJ. 2004. Structure activity relationship of oleanane disaccharides isolated from Akebia quinata versus cytotoxicity against cancer cells and NO inhibition. Biol Pharm Bull 27: 744-747. https://doi.org/10.1248/bpb.27.744
  10. Rim AR, Kim SJ, Jeon KI, Park EJ, Park HR, Lee SC. 2006. Antioxidant activity of extracts from Akebia quinata decne. J Food Sci Nutr 11: 84-87. https://doi.org/10.3746/jfn.2006.11.1.084
  11. Kim JB, Park JR, Jeon JR, Cha MH. 2001. Isolation and identification of anticancer compounds from Eucommia ulmoides leaves. J Korean Soc Food Sci Nutr 30: 732-738.
  12. Deyama T, Ikawa T, Kitagawa S, Nishibe S. 1987. The constituents of Eucommia ulmoides Oliv. VI. Isolation of a new sesquilignan and nelolignan glycosides. Chem Pharm Bull 35: 1803-1807. https://doi.org/10.1248/cpb.35.1803
  13. Hong ND, Rho YS, Kim JW, Won DH, Kim NJ, Cho BS. 1988. Studies on the general pharmacological activities of Euocommia ulmoides Oliver. Korean J Pharmacogn 19: 102-110.
  14. Cho SW, Kim YK. 2009. Studies on protective effect of Bupleurum falcatum extract (SHI-1909) against experimental inflammtory bowel disease model. J ournal of the Korea Academia-Industrial Cooperation Society 10: 613-619. https://doi.org/10.5762/KAIS.2009.10.3.613
  15. Sonwa MM, Konig WA. 2001. Chemical study of the essential oil of Cyperus rotundus. Phytochemistry 58: 799-810. https://doi.org/10.1016/S0031-9422(01)00301-6
  16. Hwang EY, Kim DH, Kim HJ, Hwang JY, Park TS, Lee IS, Son JH. 2011. Antioxidant activities and nitric oxide production of medicine plants in Gyeongsangbukdo (Carthamus tinctorius seed, Cyperus rotundus, Schizonepeta tenuifolia, Polygonatum odoratum var. pluriflorum, Paeonia lactiflora). J Appl Biol Chem 54: 171-177. https://doi.org/10.3839/jabc.2011.029
  17. Cho YH, Lee BC, Kim JH, Kim JH, Pyo HB, Zhang YH, Park HD. 2005. Effect of Artemisia anomala S. Moore on antioxidant activity and melanogenesis. Korean J Pharmacogn 36: 273-277.
  18. Choi SY, Lim SH, Kim JS, Ha TY, Kim SR, Kang KS, Hwang IK. 2005. Evaluation of the estrogenic and antioxidant activity of some edible and medicinal plants. Korean J Food Sci Technol 37: 549-556.
  19. Kim DH, Kim HJ, Chung BW. 2006. Extraction of anti-oxidative substance from Haematococcus pluvialis using ultrasonification. J Eng Res 37: 79-86.
  20. Kim HY, Woo KS, Hwang IG, Lee YR, Jeong HS. 2008. Effects of heat treatments on the antioxidant activities of fruits and vegetables. Korean J Food Sci Technol 40: 166-170.
  21. Choi Y, Lee SM, Chun J, Lee HB, Lee J. 2006. Influence of heat treatment on the antioxidant activities and polyphenolic compounds of Shiitake (Lentinus edodes) mushroom. Food Chem 99: 381-387. https://doi.org/10.1016/j.foodchem.2005.08.004
  22. Yang SJ, Woo KS, Yoo JS, Kang TS, Noh YH, Lee JS, Jeong HS. 2006. Change pf Korean ginseng components with high temperature and pressure treatment. Korean J Food Sci Technol 38: 521-525.
  23. Woo KS, Jang KI, Kim KY, Lee HB, Jeong HS. 2006. Antioxidative activity of heat treated licorice (Glycyrrhiz uralensis Fisch) extracts. Korean J Food Sci Technol 38: 355-360.
  24. Hwang IG, Woo KS, Kim TM, Kim DJ, Yang MH, Jeong HS. 2006. Change of physicochemical characteristics of Korean pear (Pyrus pyrifolia Nakai) juice with heat treatment conditions. Korean J Food Sci Technol 38: 342-347.
  25. Kwon OC, Woo KS, Kim TM, Kim DJ, Hong JT, Jeong HS. 2006. Physicochemical characteristics of garlic (Allium sativum L.) on the high temperature and pressure treatment. Korean J Food Sci Technol 38: 331-336.
  26. Chung IM, Kim KH, Ahan JK. 1998. Screening of Korean medicinal and food plants with antioxidant activity. Korean J Medicinal Crop Sci 6: 311-322.
  27. Lee SE, Seong NS, Bang JK, Park CG, Sung JS, Song J. 2003. Antioxidative activities of Korean medicinal plants. Korean J Medicinal Crop Sci 11: 127-134.
  28. Dewanto V, Wu X, Adom KK, Liu RH. 2002. Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. J Agric Food Chem 50: 3010-3014. https://doi.org/10.1021/jf0115589
  29. Jia Z, Tang M, Wu J. 1999. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 64: 555-559 https://doi.org/10.1016/S0308-8146(98)00102-2
  30. Choi Y, Kim MH, Shin JJ, Park JM, Lee J. 2003. The antioxidant activities of the some commercial teas. J Korean Soc Food Sci Nutr 32: 723-727. https://doi.org/10.3746/jkfn.2003.32.5.723
  31. Mau JL, Lin HC, Song SF. 2002. Antioxidant properties of several specialty mushrooms. Food Res Int 35: 519-526. https://doi.org/10.1016/S0963-9969(01)00150-8
  32. Turkmen N, Ferda SY, Sedat V. 2005. The effect of cooking methods on total phenolics and antioxidant activity of selected green vegetables. Food Chem 93: 713-718. https://doi.org/10.1016/j.foodchem.2004.12.038
  33. Woo JH, Shin SL, Jeong HS, Lee CH. 2010. Influence of applied pressure and heat treatment on antioxidant activities of young leaves from Achillea alpina and Solidago virgaurea subsp. gigantea. Korean J Plant Res 23: 123-130.
  34. Yu JS, Woo KS, Hwang IG, Chang YD, Jeong HJ, Lee CH, Jeong HS. 2008. Quality characteristics of Chrysanthemum indicum L. flower tea in relation to the number of panfiring. J Korean Soc Food Sci Nutr 37: 647-652. https://doi.org/10.3746/jkfn.2008.37.5.647
  35. Hong Mj, Lee GD, Kim HK, Kwon JH. 1998. Changes in functional and sensory properties of chicory roots induced by roasting processes. Korean J Food Sci Technol 30: 413-418.
  36. Zhong G, Roledo RT, Chen Z. 2003. Effects of heat treatment on flavonoids content and antioxidant capacity of buckwheat (Fagopyrum tataricum Gaert) flour ethanolic extracts. Agric Sci China 2: 1035-1040.
  37. Kim MJ, Choi JS, Song EJ, Lee SY, Kim KBWR, Lee SJ, Kim SJ, Yoon SY, Jeon YJ, Ahn DH. 2009. Effects of heat and pH treatments on antioxidant properties of Ishige okamurai extract. Korean J Food Sci Technol 41: 50-56.
  38. Kwon GJ, Choi DS, Wang MH. 2007. Biological activities of hot water extracts from Euonymus alatus leaf. Korean J Food Sci Technol 39: 569-574.
  39. Yoshino M, Murakami K. 1998. interaction of iron with polyphenolic compounds, application to antioxidant characterization. Anal Biochem 257: 40-44. https://doi.org/10.1006/abio.1997.2522
  40. Rim AR, Jung ES, Kim SY, Lee SC. 2005. Effect of far-infrared irradiation and heat treatment on the antioxidant activity of extracts from defatted soybean meal. Korean J Soc Appl Biol Chem 48: 400-403.
  41. Lee SH, Hwang IG, Lee YR, Joung EM, Jeong HS, Lee HB. 2009. Physicochemical characteristics and antioxidant activity of heated radish (Raphanus sativus L.) extracts. J Korean Soc Food Sci Nutr 38: 490-495. https://doi.org/10.3746/jkfn.2009.38.4.490

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