Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지
DOI QR코드

DOI QR Code

Antioxidant Activities of Extract Fractions of Leaves from Loquat (Eriobotrya japonica Lindl.) by Cultivars

비파의 품종별 항산화 활성 평가

  • Shin, Hyun-Jin (Research Institute of Veterinary Medicine, Chungnam National University) ;
  • Kim, Kyoung-Hee (Dept. of Food and Nutrition, Chungnam National University) ;
  • Hwang, Hye-Rim (Dept. of Food and Nutrition, Chungnam National University) ;
  • Kim, Na-Young (Dept. of Food and Nutrition, Jungbu University) ;
  • Kim, Sung-Hwan (Dept. of Food and Nutrition, Jungbu University) ;
  • Yook, Hong-Sun (Dept. of Food and Nutrition, Chungnam National University)
  • 신현진 (충남대학교 수의학과) ;
  • 김경희 (충남대학교 식품영양학과) ;
  • 황혜림 (충남대학교 식품영양학과) ;
  • 김나영 (중부대학교 식품영양학과) ;
  • 김성환 (중부대학교 식품영양학과) ;
  • 육홍선 (충남대학교 식품영양학과)
  • Received : 2012.03.28
  • Accepted : 2012.05.30
  • Published : 2012.08.31
Download PDF
⟨ Previous Next ⟩

Abstract

In this study, we compared the antioxidant activities of three loquat (Eriobotrya japonica Lindl.) by cultivars (Daebang, Bubang and native cultivar). The leaves were extracted by 80% ethanol and then fractionated with various solvents (n-hexane, ethyl acetate, n-butanol, and water) for biological activities and bioactive compounds analysis. Total polyphenol content of extracts and fractions was in the range of 84.93~478.50 mg/g gallic acid equivalent (GAE). From highest to lowest GAE, the fractions were n-butanol> ethyl acetate> n-hexane> 80% ethanol> water. Among the three cultivars, the highest polyphenol content was found from native cultivar. As for DPPH radical scavenging activity, the n-butanol fraction showed the highest activity, and native cultivar was the highest on the $IC_{50}$ values (0.18 mg/mL). In the nitrite scavenging activity, the ethyl acetate fraction (54.99~60.86%) showed the most effective activity on the Bubang cultivar was higher than others. The ursolic acid content of the ethyl acetate fraction showed the highest (51.41 mg/g) in the Daebang cultivar. Based on all these results;the Bubang cultivar showed relatively higher antioxidant and nitrite scavenging activities, but the ursolic acid content was higher in the Daebang cultivar. These results suggest that extracts from loquats (Eriobotrya japonica Lindl.) can be used as bioactive and functional materials that could be important information for industrial use in the future.

본 연구에서는 비파잎의 품종 중에서 대방, 부방, 재래종의 비파잎을 80% 에탄올로 추출하여 농축한 후 이를 여러 용매로 추출 분획하고 분리한 다음 이 분획들의 품종에 따른 비파잎의 ursolic acid 함량을 분석하고 품종 간 생리활성을 비교 분석하였다. 비파잎 에탄올 추출물 및 분획물의 총 폴리페놀 화합물 함량은 84.93~478.5 mg/g GAE로 부탄올 분획물이 가장 높은 함량을 보였고 에틸아세테이트, 헥산, 80% 에탄올, 물 분획물 순으로 나타났다. 3품종 중에서 총 폴리페놀 화합물이 가장 높은 품종은 재래종 품종이었다. DPPH 라디칼 소거능은 3품종 모두 부탄올 분획물이 가장 높았으며, 3품종 중에서 재래종 품종의 $IC_{50}$값이 0.18 mg/mL로 분석되어 각 분획물 및 3품종 중 가장 높은 DPPH 라디칼 소거능을 나타내었다. 아질산염 소거능은 3품종 모두 에틸아세테이트(54.99~60.86%) 분획물이 가장 높은 활성을 나타냈으며, 3품종 중 부방 품종의 활성이 가장 높게 나타났다. 비파잎의 에틸아세테이트 분획물의 ursolic acid 함량은 3품종 중에서 대방 품종이 51.41 mg/g으로 가장 높게 분석되었다(부방: 39.85 mg/g, 재래종: 31.13 mg/g). 이상의 연구 결과로 비파잎의 80% 에탄올 추출물 및 용매 분획물은 부방 품종에서 비교적 높은 항산화 활성 및 아질산염 소거활성을 지니고 있으며, ursolic acid 함량은 대방 품종에서 높은 함량을 가지는 것으로 분석되었다. 따라서 유효한 생리활성을 나타낸 품종별 비파잎에 대한 연구는 비파잎의 산업적 이용에 효율적인 자료로 이용될 것이다.

Keywords

References

  1. Lee CB. 1982. Korean Pictorial Book of Plants. Hyangmoonsa, Seoul, Korea. p 684-687.
  2. Shimizu M, Uemitsu N, Shirota M, Matsumato K, Tezuka Y. 1996. A new triterpene ester from Eriobotrya japonica. Chem Pharm Bull 44: 2192-2182. https://doi.org/10.1248/cpb.44.2192
  3. Nozatto N, Matsumoto K, Uemitsu N. 1994. Triterpene from leaves of Eriobotrya japonica. Nat Ned 48: 336.
  4. Jung HA, Pard JC, Chung HY, Kim J, Choi JS. 1999. Antioxidant flavonoids and chlorogenic acid from the leaves of Eriobotrya japonica. Arch Pharm Res 22: 213-218. https://doi.org/10.1007/BF02976549
  5. Ito H, Kobayashi E, Li SH, Hatano T, Sugita D, Shimura S, Itoh Y, Tokuda H, Nishino H, Yoshida T. 2002. Antitumor activity of compounds isolated from leaves of Eriobotrya japonica. J Agric Food Chem 50: 2400-2403. https://doi.org/10.1021/jf011083l
  6. De Tommasi N, De Simone F, Pizza C, Mahmood N, Moore PS, Coti C, Orsi N, Stein ML. 1992. Constituents of Eriobotrya japonica. A study of their antiviral properties. J Nat Prod 55: 1067-1073. https://doi.org/10.1021/np50086a006
  7. Choi JS, Park DY, Moon SH, Rhee SH, Young HS. 1994. Antimutagenic effect of plant flavonoids in the Salmonella assay system. Arch Pharm Res 17: 71-75. https://doi.org/10.1007/BF02974226
  8. Hamada A, Yoshioka S, Takuma D, YoKota J, Cui T, Kusunose M, Miyamura M, Kyotani S, Nishika Y. 2004. The effect of Eriobotrya japonica seed extract on oxidative stress in adriamycin-induced nephropathy in rats. Biol Pharm Bull 27: 1961-1964. https://doi.org/10.1248/bpb.27.1961
  9. Yokota J, Daisuke T, Hamada A, Masahide O, Saburo Y, Kusunose M, Miyamura M, Kyotani S, Nishioka Y. 2006. Scavenging of reactive oxygen species by Eriobotrya japonica seed extract. Biol Pharm Bull 29: 467-471. https://doi.org/10.1248/bpb.29.467
  10. Koba K, Matsuoka A, Osada K, Huang YS. 2007. Effect of loquat (Eriobotrya japonica) extracts on LDL oxidation. Food Chem 104: 308-316. https://doi.org/10.1016/j.foodchem.2006.11.043
  11. Chen J, Li WL, Wu JL, Ren BR, Zhang HQ. 2008. Hypoglycemic effects of a sesquiterpene glycoside isolated from leaves of loquat (Eriobotrya japonica (Thunb.) Lindl.). Phytomedicine 15: 98-102. https://doi.org/10.1016/j.phymed.2006.12.014
  12. Banno N, Akihisa T, Tokuda H, Yasukawa K, Taguchi Y, Akazawa H, Ukiya M, Kimura Y, Suzuki T, Nishino H. 2005. Anti-inflammatory and antitumor-promoting effect of the triterpene acid from the leaves of Eriobotrya japonica. Biol Pharm Bull 28: 1995-1999. https://doi.org/10.1248/bpb.28.1995
  13. Cho YS, Park SK, Lee HY. 1991. Composition of free sugars, organic acids and free amino acids in loquat flesh. J Korean Soc Food Nutr 20: 89-93.
  14. Lee BY, Park EM, Kim EJ, Choi HD, Kim IH, Hwang JB. 1996. Analysis of chemical components of Korean loquat (Eriobotrya japonica Lindl.) fruit. Korean J Food Sci Technol 28: 428-432.
  15. Bae YI, Shim KH. 1998. Nutrition components in different parts of Korean loquat (Eriobotrya japonica Lindl.). Korean J Pstharv Sci Technol 5: 57-63.
  16. Bae YI, Chung YC, Shim KH. 2002. Antimicrobial and antioxidant activities of various solvent extract from different parts of loquat (Eriobotrya japonica Lindl.). Korean J Food Preserv 9: 97-101.
  17. Bae YI, Jeong CH, Shim KH. 2005. Antioxidative and antimicrobial activity of epicatechin isolated from leaves of loquat (Eriobotrya japonica). J Food Sci Nutr 10: 118-121. https://doi.org/10.3746/jfn.2005.10.2.118
  18. Soung DY, Kim JS, Chung HY, Jung HA, Park JC, Choi JS. 1999. Flavonoids and chlorogenic acid from Eriobotrya japonica scavenge peroxynitrite. Nat Prod Sci 5: 80-84.
  19. Hong Y, Lin S, Jiang Y, Ashraf M. 2008. Variation in contents of total phenolics and flavonoids and antioxidant activities in the leaves of 11 Eriobotrya species. Plant Foods Hum Nutr 63: 200-204. https://doi.org/10.1007/s11130-008-0088-6
  20. Lee KI, Park MY, Pyo BS, Kim SM. 2010. Development of an effective extraction method for the quality control of eriobotrae folium: determination of triterpenic acids. Kor J Pharmacogn 41: 62-66.
  21. Folin O, Denis W. 1912. On phosphotungastic-phosphomolybdic compounds as color reagents. J Biol Chem 12: 239- 249.
  22. Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181: 1198-1200.
  23. Gray JI, Dugan Jr LR. 1975. Inhibition of N-nitrosamine formation in model food systems. J Food Sci 40: 981-984. https://doi.org/10.1111/j.1365-2621.1975.tb02248.x
  24. Fruit Crops Experiment Station. http://nanji.jares.go.kr/html/technique/medlar02.htm
  25. Lee KI, Kim SM. 2009. Antioxidative and antimicrobial activities of Eriobotrya japonica Lindl. leaf extracts. J Korean Soc Food Sci Nutr 38: 267-273. https://doi.org/10.3746/jkfn.2009.38.3.267
  26. Jeong YS, Jung HK, Youn KS, Kim MO, Hong JH. 2009. Physiological activities of the hot water extract from Eriobotrya japonica Lindl.. J Korean Soc Food Sci Nutr 38: 977-982. https://doi.org/10.3746/jkfn.2009.38.8.977
  27. Bang IS, Park HY, Yuh CS, Kim AJ, Yu CY, Chimire B, Lee HS, Park JG, Choung MG, Lim JD. 2007. Antioxidant activities and phenolic compounds composition of extracts from mulberry (Morus alba L.) fruit. Korean J Med Crop Sci 15: 120-127.
  28. Shim KH. 1998. Studies on the chemical compounds, biological activities and utilization of loquat in Korea. Report National Research Foundation of Korea (961-0605-037-2). p 19.
  29. Seo YH, Kim IJ, Yie AS, Min HK. 1999. Electron donating ability and contents of phenolic compounds, tocopherols and carotenoid in waxy corn (Zea mays L.). Korean J Food Sci Technol 31: 581-585.
  30. Lee SE, Kim YS, Kim JE, Bang JK, Seong NS. 2004. Antioxidant activity of Ulmus davidiana var. japonica N. and Hemipteleae davidii P. J Korean Med Crop Sci 12: 321-327.
  31. Park YS, Park YJ, Kim HJ, Im MH, Lee MK, Kim YM, Cho JY, Heo BG. 2008. Physiological activity of ethanol extract from the different plant parts of loquat (Eriobotrya japonica Lindl.). Kor J Hort Sci Technol 26: 75-80.
  32. Kang YH, Park YK, OH SR, Moon KD. 1995. Studies on the physiological functionality of pine meddle and mugwort extracts. Korean J Food Sci Technol 27: 978-984.
  33. Kytopoulos SA. 1987. Ascorbic acid and formation of N-nitroso compounds: possible role of ascorbic acid in cancer prevention. Am J Clin Nutr 45: 1344-1350.

Cited by

  1. Quality Characteristics of Jeolpyun with Different Ratios of Loquat Leaf Powder     vol.25, pp.5, 2015, https://doi.org/10.17495/easdl.2015.10.25.5.842
  2. Antioxidant Activities and Quality Characteristic of Jeung-Pyun with the Addition of Loquat Leaf Powder vol.50, pp.10, 2021, https://doi.org/10.3746/jkfn.2021.50.10.1065
  3. Correlation of the free radical and antioxidant activities of Eriobotrya Japonica Lindl. with phenolic and flavonoid contents vol.41, pp.4, 2012, https://doi.org/10.1590/fst.21720